ProMMP-1 PRODUCTION BY CULTIVATED CELLS OF VASCULAR ENDОTHELIUM IN VITRO AND IN A HUMAN BODY

Abstract. Matrix metalloproteinases (MMP) are structurally related endopeptidase composed of active sites which include ions Zn2+ and Ca2+. Cultured cells of human blood vessels produce MMP-1, proteolytic effect is aimed at splitting the collagen I and III types, and subsequent vascular remodeling....

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Autores principales:	N. N. Scliankina, N. V. Boldyreva, A. A. Babayants, I. S. Frolova, D. L. Belyaev, Yu. A. Portnova, O. N. Scheglovitova
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2014
Materias:	prommp-1 vascular endothelium huvec interferon immunomodulators Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/8dfc60981add4d9db891648d96ccfae3
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spelling	oai:doaj.org-article:8dfc60981add4d9db891648d96ccfae32021-11-22T07:09:45ZProMMP-1 PRODUCTION BY CULTIVATED CELLS OF VASCULAR ENDОTHELIUM IN VITRO AND IN A HUMAN BODY2220-76192313-739810.15789/2220-7619-2011-3-271-274https://doaj.org/article/8dfc60981add4d9db891648d96ccfae32014-06-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/58https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Abstract. Matrix metalloproteinases (MMP) are structurally related endopeptidase composed of active sites which include ions Zn2+ and Ca2+. Cultured cells of human blood vessels produce MMP-1, proteolytic effect is aimed at splitting the collagen I and III types, and subsequent vascular remodeling. MMP-1 is synthesized as an inactive zymogen proMMP-1. It was shown that interferon alpha, beta and gamma inhibited production by culture of HUVEC proenzyme MMP-1, which seems to characterize their anti-angiogenic action. The effect of immunomodulators is more difficult to explain: perhaps inhibiting effect of imunofan and, as well as activating effect of cycloferon due to their internal structural peculiarities. The action of interferon alpha, beta and gamma, used as HUVEC before infection with HSV-1, and after it led to decrease in production proMMP-1. Apparently, the antiangiogenic effect of IFN is saved in the case of infection of cultures of vascular endothelium with HSV-1. Scatter in the content of proMMP-1 in the serum of blood donors was 1.625–11.8 ng/ml and in patients with chronic microbial-viral infections was 1.22–21.16 ng/ml. Higher rates of proMMP-1 were in older patients group. To estimate the system of MMP in vitro, and in the body a comprehensive study must be conducted, including proMMP-1, the active form of proenzyme and specific inhibitor of MMP-1.N. N. ScliankinaN. V. BoldyrevaA. A. BabayantsI. S. FrolovaD. L. BelyaevYu. A. PortnovaO. N. ScheglovitovaSankt-Peterburg : NIIÈM imeni Pasteraarticleprommp-1vascular endotheliumhuvecinterferonimmunomodulatorsInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 1, Iss 3, Pp 271-274 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	prommp-1 vascular endothelium huvec interferon immunomodulators Infectious and parasitic diseases RC109-216
spellingShingle	prommp-1 vascular endothelium huvec interferon immunomodulators Infectious and parasitic diseases RC109-216 N. N. Scliankina N. V. Boldyreva A. A. Babayants I. S. Frolova D. L. Belyaev Yu. A. Portnova O. N. Scheglovitova ProMMP-1 PRODUCTION BY CULTIVATED CELLS OF VASCULAR ENDОTHELIUM IN VITRO AND IN A HUMAN BODY
description	Abstract. Matrix metalloproteinases (MMP) are structurally related endopeptidase composed of active sites which include ions Zn2+ and Ca2+. Cultured cells of human blood vessels produce MMP-1, proteolytic effect is aimed at splitting the collagen I and III types, and subsequent vascular remodeling. MMP-1 is synthesized as an inactive zymogen proMMP-1. It was shown that interferon alpha, beta and gamma inhibited production by culture of HUVEC proenzyme MMP-1, which seems to characterize their anti-angiogenic action. The effect of immunomodulators is more difficult to explain: perhaps inhibiting effect of imunofan and, as well as activating effect of cycloferon due to their internal structural peculiarities. The action of interferon alpha, beta and gamma, used as HUVEC before infection with HSV-1, and after it led to decrease in production proMMP-1. Apparently, the antiangiogenic effect of IFN is saved in the case of infection of cultures of vascular endothelium with HSV-1. Scatter in the content of proMMP-1 in the serum of blood donors was 1.625–11.8 ng/ml and in patients with chronic microbial-viral infections was 1.22–21.16 ng/ml. Higher rates of proMMP-1 were in older patients group. To estimate the system of MMP in vitro, and in the body a comprehensive study must be conducted, including proMMP-1, the active form of proenzyme and specific inhibitor of MMP-1.
format	article
author	N. N. Scliankina N. V. Boldyreva A. A. Babayants I. S. Frolova D. L. Belyaev Yu. A. Portnova O. N. Scheglovitova
author_facet	N. N. Scliankina N. V. Boldyreva A. A. Babayants I. S. Frolova D. L. Belyaev Yu. A. Portnova O. N. Scheglovitova
author_sort	N. N. Scliankina
title	ProMMP-1 PRODUCTION BY CULTIVATED CELLS OF VASCULAR ENDОTHELIUM IN VITRO AND IN A HUMAN BODY
title_short	ProMMP-1 PRODUCTION BY CULTIVATED CELLS OF VASCULAR ENDОTHELIUM IN VITRO AND IN A HUMAN BODY
title_full	ProMMP-1 PRODUCTION BY CULTIVATED CELLS OF VASCULAR ENDОTHELIUM IN VITRO AND IN A HUMAN BODY
title_fullStr	ProMMP-1 PRODUCTION BY CULTIVATED CELLS OF VASCULAR ENDОTHELIUM IN VITRO AND IN A HUMAN BODY
title_full_unstemmed	ProMMP-1 PRODUCTION BY CULTIVATED CELLS OF VASCULAR ENDОTHELIUM IN VITRO AND IN A HUMAN BODY
title_sort	prommp-1 production by cultivated cells of vascular endоthelium in vitro and in a human body
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2014
url	https://doaj.org/article/8dfc60981add4d9db891648d96ccfae3
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ProMMP-1 PRODUCTION BY CULTIVATED CELLS OF VASCULAR ENDОTHELIUM IN VITRO AND IN A HUMAN BODY

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