Non-Invasive Physical Plasma Enhances the Membrane Permeability to Low Molecular Weight Compounds and Subsequently Leads to the Loss of Cellular ATP and the Devitalization of Epithelial Cancer Cells

Non-invasive physical plasma (NIPP) achieves biomedical effects primarily through the formation of reactive oxygen and nitrogen species. In clinical use, these species interact with cells of the treated tissue, affecting the cytoplasmic membrane first. The present study investigated the permeability...

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Autores principales:	Caroline Sander, Andreas Nitsch, Holger H. H. Erb, Eva K. Egger, Lyubomir Haralambiev, Benedikt Eggers, Franz-Josef Kramer, Martin Weiss, Alexander Mustea, Matthias B. Stope
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	physical plasma non-invasive mamma carcinoma breast cancer cytoplasmic membrane chemo-sensitivity Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/8e0832b41e054f09b38092a5ff565c9c
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spelling	oai:doaj.org-article:8e0832b41e054f09b38092a5ff565c9c2021-11-11T14:57:40ZNon-Invasive Physical Plasma Enhances the Membrane Permeability to Low Molecular Weight Compounds and Subsequently Leads to the Loss of Cellular ATP and the Devitalization of Epithelial Cancer Cells10.3390/app112198012076-3417https://doaj.org/article/8e0832b41e054f09b38092a5ff565c9c2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/9801https://doaj.org/toc/2076-3417Non-invasive physical plasma (NIPP) achieves biomedical effects primarily through the formation of reactive oxygen and nitrogen species. In clinical use, these species interact with cells of the treated tissue, affecting the cytoplasmic membrane first. The present study investigated the permeability of the cytoplasmic membrane of breast cancer cells with different fluorescent dyes after NIPP treatment and determined the subsequent effects on cell viability. After NIPP treatment and the associated formation of reactive oxygen species, low molecular weight compounds were able to pass through the cytoplasmic membrane in both directions to a higher extent. Consequently, a loss of cellular ATP into the extracellular space was induced. Due to these limitations in cell physiology, apoptosis was induced in the cancer cells and the entire cell population exhibited decreased cell growth. It can be concluded that NIPP treatment disturbs the biochemical functionality of the cytoplasmic membrane of cancer cells, which massively impairs their viability. This observation opens a vast application horizon of NIPP therapy to treat precancerous and malignant diseases beyond breast cancer therapy.Caroline SanderAndreas NitschHolger H. H. ErbEva K. EggerLyubomir HaralambievBenedikt EggersFranz-Josef KramerMartin WeissAlexander MusteaMatthias B. StopeMDPI AGarticlephysical plasmanon-invasivemamma carcinomabreast cancercytoplasmic membranechemo-sensitivityTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 9801, p 9801 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	physical plasma non-invasive mamma carcinoma breast cancer cytoplasmic membrane chemo-sensitivity Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	physical plasma non-invasive mamma carcinoma breast cancer cytoplasmic membrane chemo-sensitivity Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Caroline Sander Andreas Nitsch Holger H. H. Erb Eva K. Egger Lyubomir Haralambiev Benedikt Eggers Franz-Josef Kramer Martin Weiss Alexander Mustea Matthias B. Stope Non-Invasive Physical Plasma Enhances the Membrane Permeability to Low Molecular Weight Compounds and Subsequently Leads to the Loss of Cellular ATP and the Devitalization of Epithelial Cancer Cells
description	Non-invasive physical plasma (NIPP) achieves biomedical effects primarily through the formation of reactive oxygen and nitrogen species. In clinical use, these species interact with cells of the treated tissue, affecting the cytoplasmic membrane first. The present study investigated the permeability of the cytoplasmic membrane of breast cancer cells with different fluorescent dyes after NIPP treatment and determined the subsequent effects on cell viability. After NIPP treatment and the associated formation of reactive oxygen species, low molecular weight compounds were able to pass through the cytoplasmic membrane in both directions to a higher extent. Consequently, a loss of cellular ATP into the extracellular space was induced. Due to these limitations in cell physiology, apoptosis was induced in the cancer cells and the entire cell population exhibited decreased cell growth. It can be concluded that NIPP treatment disturbs the biochemical functionality of the cytoplasmic membrane of cancer cells, which massively impairs their viability. This observation opens a vast application horizon of NIPP therapy to treat precancerous and malignant diseases beyond breast cancer therapy.
format	article
author	Caroline Sander Andreas Nitsch Holger H. H. Erb Eva K. Egger Lyubomir Haralambiev Benedikt Eggers Franz-Josef Kramer Martin Weiss Alexander Mustea Matthias B. Stope
author_facet	Caroline Sander Andreas Nitsch Holger H. H. Erb Eva K. Egger Lyubomir Haralambiev Benedikt Eggers Franz-Josef Kramer Martin Weiss Alexander Mustea Matthias B. Stope
author_sort	Caroline Sander
title	Non-Invasive Physical Plasma Enhances the Membrane Permeability to Low Molecular Weight Compounds and Subsequently Leads to the Loss of Cellular ATP and the Devitalization of Epithelial Cancer Cells
title_short	Non-Invasive Physical Plasma Enhances the Membrane Permeability to Low Molecular Weight Compounds and Subsequently Leads to the Loss of Cellular ATP and the Devitalization of Epithelial Cancer Cells
title_full	Non-Invasive Physical Plasma Enhances the Membrane Permeability to Low Molecular Weight Compounds and Subsequently Leads to the Loss of Cellular ATP and the Devitalization of Epithelial Cancer Cells
title_fullStr	Non-Invasive Physical Plasma Enhances the Membrane Permeability to Low Molecular Weight Compounds and Subsequently Leads to the Loss of Cellular ATP and the Devitalization of Epithelial Cancer Cells
title_full_unstemmed	Non-Invasive Physical Plasma Enhances the Membrane Permeability to Low Molecular Weight Compounds and Subsequently Leads to the Loss of Cellular ATP and the Devitalization of Epithelial Cancer Cells
title_sort	non-invasive physical plasma enhances the membrane permeability to low molecular weight compounds and subsequently leads to the loss of cellular atp and the devitalization of epithelial cancer cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8e0832b41e054f09b38092a5ff565c9c
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Non-Invasive Physical Plasma Enhances the Membrane Permeability to Low Molecular Weight Compounds and Subsequently Leads to the Loss of Cellular ATP and the Devitalization of Epithelial Cancer Cells

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