BRAF Mutation Status in Primary, Recurrent, and Metastatic Malignant Melanoma and Its Relation to Histopathological Parameters

Background: BRAF mutations are a common finding in malignant melanoma (MM). Nevertheless, apart from their significance as a therapeutic target in advanced melanoma, their prognostic value is still debated. Objective: To assess BRAF mutation status in primary, recurrent, or metastatic MM and its co...

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Autores principales:	Aris Spathis, Alexander C Katoulis, Vasileia Damaskou, Aikaterini I. Liakou, Christine Kottaridi, Danai Leventakou, Dimitrios Sgouros, Andreas Mamantopoulos, Dimitrios Rigopoulos, Petros Karakitsos, Ioannis G. Panayiotides
Formato:	article
Lenguaje:	EN
Publicado:	Mattioli1885 2019
Materias:	malignant melanoma histopathological features BRAF mutations Dermatology RL1-803
Acceso en línea:	https://doaj.org/article/8e09263f893b4a9ebb331dde7251d641
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spelling	oai:doaj.org-article:8e09263f893b4a9ebb331dde7251d6412021-11-17T08:29:49ZBRAF Mutation Status in Primary, Recurrent, and Metastatic Malignant Melanoma and Its Relation to Histopathological Parameters10.5826/dpc.0901a132160-9381https://doaj.org/article/8e09263f893b4a9ebb331dde7251d6412019-01-01T00:00:00Zhttp://dpcj.org/index.php/dpc/article/view/539https://doaj.org/toc/2160-9381 Background: BRAF mutations are a common finding in malignant melanoma (MM). Nevertheless, apart from their significance as a therapeutic target in advanced melanoma, their prognostic value is still debated. Objective: To assess BRAF mutation status in primary, recurrent, or metastatic MM and its correlations with histopathological findings. Methods: We analyzed 203 samples from 178 consecutive patients: 129 primary cutaneous MM, 49 metastatic and recurrent MM of unknown primary site, and 25 cases of recurrences or metastases of primary MM. BRAF mutations in exon 15 were identified with real-time polymerase chain reaction and/or direct sequencing or pyrosequencing. Histopathological examination was performed according to standard procedures. Results: We observed a 42.1% prevalence of BRAF mutations at codon 600 among our patients, 84% of whom harbored the V600E mutation. Mutations showed a statistically significant increase in younger patients (P = 0.011), in ulcerated tumors (P = 0.020), and in tumors lacking solar elastosis in adjacent dermis (P = 0.008). Mutations were also more common in male patients, as well as in primary MMs of the torso, and in nonvisceral metastases, however without reaching statistical significance. Logistic regression analysis identified type and ulceration as the only significant predictors of BRAF mutation. The highest frequencies of mutated BRAF were identified in superficial spreading and nodular types, and the lowest in acral lentiginous and lentigo maligna types. In situ MM and primary dermal melanoma displayed intermediate frequencies. Conclusion: Frequency of mutated BRAF is type-related and correlated with ulceration, a known adverse prognostic factor. Aris SpathisAlexander C KatoulisVasileia DamaskouAikaterini I. LiakouChristine KottaridiDanai LeventakouDimitrios SgourosAndreas MamantopoulosDimitrios RigopoulosPetros KarakitsosIoannis G. PanayiotidesMattioli1885articlemalignant melanomahistopathological featuresBRAF mutationsDermatologyRL1-803ENDermatology Practical & Conceptual, Vol 9, Iss 1 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	malignant melanoma histopathological features BRAF mutations Dermatology RL1-803
spellingShingle	malignant melanoma histopathological features BRAF mutations Dermatology RL1-803 Aris Spathis Alexander C Katoulis Vasileia Damaskou Aikaterini I. Liakou Christine Kottaridi Danai Leventakou Dimitrios Sgouros Andreas Mamantopoulos Dimitrios Rigopoulos Petros Karakitsos Ioannis G. Panayiotides BRAF Mutation Status in Primary, Recurrent, and Metastatic Malignant Melanoma and Its Relation to Histopathological Parameters
description	Background: BRAF mutations are a common finding in malignant melanoma (MM). Nevertheless, apart from their significance as a therapeutic target in advanced melanoma, their prognostic value is still debated. Objective: To assess BRAF mutation status in primary, recurrent, or metastatic MM and its correlations with histopathological findings. Methods: We analyzed 203 samples from 178 consecutive patients: 129 primary cutaneous MM, 49 metastatic and recurrent MM of unknown primary site, and 25 cases of recurrences or metastases of primary MM. BRAF mutations in exon 15 were identified with real-time polymerase chain reaction and/or direct sequencing or pyrosequencing. Histopathological examination was performed according to standard procedures. Results: We observed a 42.1% prevalence of BRAF mutations at codon 600 among our patients, 84% of whom harbored the V600E mutation. Mutations showed a statistically significant increase in younger patients (P = 0.011), in ulcerated tumors (P = 0.020), and in tumors lacking solar elastosis in adjacent dermis (P = 0.008). Mutations were also more common in male patients, as well as in primary MMs of the torso, and in nonvisceral metastases, however without reaching statistical significance. Logistic regression analysis identified type and ulceration as the only significant predictors of BRAF mutation. The highest frequencies of mutated BRAF were identified in superficial spreading and nodular types, and the lowest in acral lentiginous and lentigo maligna types. In situ MM and primary dermal melanoma displayed intermediate frequencies. Conclusion: Frequency of mutated BRAF is type-related and correlated with ulceration, a known adverse prognostic factor.
format	article
author	Aris Spathis Alexander C Katoulis Vasileia Damaskou Aikaterini I. Liakou Christine Kottaridi Danai Leventakou Dimitrios Sgouros Andreas Mamantopoulos Dimitrios Rigopoulos Petros Karakitsos Ioannis G. Panayiotides
author_facet	Aris Spathis Alexander C Katoulis Vasileia Damaskou Aikaterini I. Liakou Christine Kottaridi Danai Leventakou Dimitrios Sgouros Andreas Mamantopoulos Dimitrios Rigopoulos Petros Karakitsos Ioannis G. Panayiotides
author_sort	Aris Spathis
title	BRAF Mutation Status in Primary, Recurrent, and Metastatic Malignant Melanoma and Its Relation to Histopathological Parameters
title_short	BRAF Mutation Status in Primary, Recurrent, and Metastatic Malignant Melanoma and Its Relation to Histopathological Parameters
title_full	BRAF Mutation Status in Primary, Recurrent, and Metastatic Malignant Melanoma and Its Relation to Histopathological Parameters
title_fullStr	BRAF Mutation Status in Primary, Recurrent, and Metastatic Malignant Melanoma and Its Relation to Histopathological Parameters
title_full_unstemmed	BRAF Mutation Status in Primary, Recurrent, and Metastatic Malignant Melanoma and Its Relation to Histopathological Parameters
title_sort	braf mutation status in primary, recurrent, and metastatic malignant melanoma and its relation to histopathological parameters
publisher	Mattioli1885
publishDate	2019
url	https://doaj.org/article/8e09263f893b4a9ebb331dde7251d641
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BRAF Mutation Status in Primary, Recurrent, and Metastatic Malignant Melanoma and Its Relation to Histopathological Parameters

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