Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12.

In addition to its role as neurotransmitter, serotonin (5-HT) is an important modulator of inflammation and immunity. Here, we report novel findings suggesting a 5-HT involvement in T cell migration. In particular, we show that 5-HT tunes the responsiveness of human T lymphocytes to the broadly expr...

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Autores principales:	Elena Magrini, Ildikò Szabò, Andrea Doni, Javier Cibella, Antonella Viola
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8e0db73ea87843669a8800029f03fce7
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spelling	oai:doaj.org-article:8e0db73ea87843669a8800029f03fce72021-11-18T06:48:22ZSerotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12.1932-620310.1371/journal.pone.0022482https://doaj.org/article/8e0db73ea87843669a8800029f03fce72011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21853036/?tool=EBIhttps://doaj.org/toc/1932-6203In addition to its role as neurotransmitter, serotonin (5-HT) is an important modulator of inflammation and immunity. Here, we report novel findings suggesting a 5-HT involvement in T cell migration. In particular, we show that 5-HT tunes the responsiveness of human T lymphocytes to the broadly expressed chemokine CXCL12 in transwell migration assays. By real-time PCR, western blot analysis and electrophysiological patch clamp experiments, we demonstrate that the type 3 5-HT receptor (5-HT(3)) is functionally expressed in human primary T cells. In addition, specific 5-HT(3) receptor agonists selectively decrease T cell migration towards gradients of CXCL12 but not of inflammatory chemokines, such as CCL2 and CCL5. In transmigration experiments, 5-HT(3) receptor stimulation reverts the inhibitory effect of endothelial-bound CXCL12 on T cell migration. Our data suggest that the reduced T cell responsiveness to CXCL12 induced by 5-HT may occur to facilitate T cell extravasation and migration into inflamed tissues.Elena MagriniIldikò SzabòAndrea DoniJavier CibellaAntonella ViolaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e22482 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elena Magrini Ildikò Szabò Andrea Doni Javier Cibella Antonella Viola Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12.
description	In addition to its role as neurotransmitter, serotonin (5-HT) is an important modulator of inflammation and immunity. Here, we report novel findings suggesting a 5-HT involvement in T cell migration. In particular, we show that 5-HT tunes the responsiveness of human T lymphocytes to the broadly expressed chemokine CXCL12 in transwell migration assays. By real-time PCR, western blot analysis and electrophysiological patch clamp experiments, we demonstrate that the type 3 5-HT receptor (5-HT(3)) is functionally expressed in human primary T cells. In addition, specific 5-HT(3) receptor agonists selectively decrease T cell migration towards gradients of CXCL12 but not of inflammatory chemokines, such as CCL2 and CCL5. In transmigration experiments, 5-HT(3) receptor stimulation reverts the inhibitory effect of endothelial-bound CXCL12 on T cell migration. Our data suggest that the reduced T cell responsiveness to CXCL12 induced by 5-HT may occur to facilitate T cell extravasation and migration into inflamed tissues.
format	article
author	Elena Magrini Ildikò Szabò Andrea Doni Javier Cibella Antonella Viola
author_facet	Elena Magrini Ildikò Szabò Andrea Doni Javier Cibella Antonella Viola
author_sort	Elena Magrini
title	Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12.
title_short	Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12.
title_full	Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12.
title_fullStr	Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12.
title_full_unstemmed	Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12.
title_sort	serotonin-mediated tuning of human helper t cell responsiveness to the chemokine cxcl12.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/8e0db73ea87843669a8800029f03fce7
work_keys_str_mv	AT elenamagrini serotoninmediatedtuningofhumanhelpertcellresponsivenesstothechemokinecxcl12 AT ildikoszabo serotoninmediatedtuningofhumanhelpertcellresponsivenesstothechemokinecxcl12 AT andreadoni serotoninmediatedtuningofhumanhelpertcellresponsivenesstothechemokinecxcl12 AT javiercibella serotoninmediatedtuningofhumanhelpertcellresponsivenesstothechemokinecxcl12 AT antonellaviola serotoninmediatedtuningofhumanhelpertcellresponsivenesstothechemokinecxcl12
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Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12.

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