Nanoparticle-based hyperthermia distinctly impacts production of ROS, expression of Ki-67, TOP2A, and TPX2, and induction of apoptosis in pancreatic cancer

Robert Ludwig,1 Francisco J Teran,2,3 Ulf Teichgraeber,1 Ingrid Hilger1 1Department of Experimental Radiology, Institute for Diagnostic and Interventional Radiology, Jena University Hospital – Friedrich Schiller University Jena, Jena, Germany; 2iMdea-Nanociencia, Campus Universitario de C...

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Autores principales:	Ludwig R, Teran FJ, Teichgraeber U, Hilger I
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	iron oxide nanoparticles magnetic hyperthermia heat dose nanomedicine proliferation marker Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8e10cfcdca304d84b61b3e57da533912
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spelling	oai:doaj.org-article:8e10cfcdca304d84b61b3e57da5339122021-12-02T00:46:39ZNanoparticle-based hyperthermia distinctly impacts production of ROS, expression of Ki-67, TOP2A, and TPX2, and induction of apoptosis in pancreatic cancer1178-2013https://doaj.org/article/8e10cfcdca304d84b61b3e57da5339122017-02-01T00:00:00Zhttps://www.dovepress.com/nanoparticle-based--hyperthermia-distinctly-impacts-production-of-ros--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Robert Ludwig,1 Francisco J Teran,2,3 Ulf Teichgraeber,1 Ingrid Hilger1 1Department of Experimental Radiology, Institute for Diagnostic and Interventional Radiology, Jena University Hospital – Friedrich Schiller University Jena, Jena, Germany; 2iMdea-Nanociencia, Campus Universitario de Cantoblanco, 3Nanobiotecnología (iMdea-Nanociencia), Unidad Asociada al Centro Nacional de Biotecnología (CSIC), Madrid, Spain Abstract: So far, the therapeutic outcome of hyperthermia has shown heterogeneous responses depending on how thermal stress is applied. We studied whether extrinsic heating (EH, hot air) and intrinsic heating (magnetic heating [MH] mediated by nanoparticles) induce distinct effects on pancreatic cancer cells (PANC-1 and BxPC-3 cells). The impact of MH (100 µg magnetic nanoparticles [MNP]/mL; H=23.9 kA/m; f=410 kHz) was always superior to that of EH. The thermal effects were confirmed by the following observations: 1) decreased number of vital cells, 2) altered expression of pro-caspases, and 3) production of reactive oxygen species, and 4) altered mRNA expression of Ki-67, TOP2A, and TPX2. The MH treatment of tumor xenografts significantly (P≤0.05) reduced tumor volumes. This means that different therapeutic outcomes of hyperthermia are related to the different responses cells exert to thermal stress. In particular, intratumoral MH is a valuable tool for the treatment of pancreatic cancers. Keywords: iron oxide nanoparticles, magnetic hyperthermia, heat dose, nanomedicine, proliferation markerLudwig RTeran FJTeichgraeber UHilger IDove Medical Pressarticleiron oxide nanoparticlesmagnetic hyperthermiaheat dosenanomedicineproliferation markerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 1009-1018 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	iron oxide nanoparticles magnetic hyperthermia heat dose nanomedicine proliferation marker Medicine (General) R5-920
spellingShingle	iron oxide nanoparticles magnetic hyperthermia heat dose nanomedicine proliferation marker Medicine (General) R5-920 Ludwig R Teran FJ Teichgraeber U Hilger I Nanoparticle-based hyperthermia distinctly impacts production of ROS, expression of Ki-67, TOP2A, and TPX2, and induction of apoptosis in pancreatic cancer
description	Robert Ludwig,1 Francisco J Teran,2,3 Ulf Teichgraeber,1 Ingrid Hilger1 1Department of Experimental Radiology, Institute for Diagnostic and Interventional Radiology, Jena University Hospital – Friedrich Schiller University Jena, Jena, Germany; 2iMdea-Nanociencia, Campus Universitario de Cantoblanco, 3Nanobiotecnología (iMdea-Nanociencia), Unidad Asociada al Centro Nacional de Biotecnología (CSIC), Madrid, Spain Abstract: So far, the therapeutic outcome of hyperthermia has shown heterogeneous responses depending on how thermal stress is applied. We studied whether extrinsic heating (EH, hot air) and intrinsic heating (magnetic heating [MH] mediated by nanoparticles) induce distinct effects on pancreatic cancer cells (PANC-1 and BxPC-3 cells). The impact of MH (100 µg magnetic nanoparticles [MNP]/mL; H=23.9 kA/m; f=410 kHz) was always superior to that of EH. The thermal effects were confirmed by the following observations: 1) decreased number of vital cells, 2) altered expression of pro-caspases, and 3) production of reactive oxygen species, and 4) altered mRNA expression of Ki-67, TOP2A, and TPX2. The MH treatment of tumor xenografts significantly (P≤0.05) reduced tumor volumes. This means that different therapeutic outcomes of hyperthermia are related to the different responses cells exert to thermal stress. In particular, intratumoral MH is a valuable tool for the treatment of pancreatic cancers. Keywords: iron oxide nanoparticles, magnetic hyperthermia, heat dose, nanomedicine, proliferation marker
format	article
author	Ludwig R Teran FJ Teichgraeber U Hilger I
author_facet	Ludwig R Teran FJ Teichgraeber U Hilger I
author_sort	Ludwig R
title	Nanoparticle-based hyperthermia distinctly impacts production of ROS, expression of Ki-67, TOP2A, and TPX2, and induction of apoptosis in pancreatic cancer
title_short	Nanoparticle-based hyperthermia distinctly impacts production of ROS, expression of Ki-67, TOP2A, and TPX2, and induction of apoptosis in pancreatic cancer
title_full	Nanoparticle-based hyperthermia distinctly impacts production of ROS, expression of Ki-67, TOP2A, and TPX2, and induction of apoptosis in pancreatic cancer
title_fullStr	Nanoparticle-based hyperthermia distinctly impacts production of ROS, expression of Ki-67, TOP2A, and TPX2, and induction of apoptosis in pancreatic cancer
title_full_unstemmed	Nanoparticle-based hyperthermia distinctly impacts production of ROS, expression of Ki-67, TOP2A, and TPX2, and induction of apoptosis in pancreatic cancer
title_sort	nanoparticle-based hyperthermia distinctly impacts production of ros, expression of ki-67, top2a, and tpx2, and induction of apoptosis in pancreatic cancer
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/8e10cfcdca304d84b61b3e57da533912
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Nanoparticle-based hyperthermia distinctly impacts production of ROS, expression of Ki-67, TOP2A, and TPX2, and induction of apoptosis in pancreatic cancer

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