Developmental regulation of diacylglycerol acyltransferase family gene expression in tung tree tissues.

Diacylglycerol acyltransferases (DGAT) catalyze the final and rate-limiting step of triacylglycerol (TAG) biosynthesis in eukaryotic organisms. DGAT genes have been identified in numerous organisms. Multiple isoforms of DGAT are present in eukaryotes. We previously cloned DGAT1 and DGAT2 genes of tu...

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Autores principales:	Heping Cao, Jay M Shockey, K Thomas Klasson, Dorselyn C Chapital, Catherine B Mason, Brian E Scheffler
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8e1143ac27e744dcb870ae6decef2ee0
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spelling	oai:doaj.org-article:8e1143ac27e744dcb870ae6decef2ee02021-11-18T08:51:24ZDevelopmental regulation of diacylglycerol acyltransferase family gene expression in tung tree tissues.1932-620310.1371/journal.pone.0076946https://doaj.org/article/8e1143ac27e744dcb870ae6decef2ee02013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24146944/?tool=EBIhttps://doaj.org/toc/1932-6203Diacylglycerol acyltransferases (DGAT) catalyze the final and rate-limiting step of triacylglycerol (TAG) biosynthesis in eukaryotic organisms. DGAT genes have been identified in numerous organisms. Multiple isoforms of DGAT are present in eukaryotes. We previously cloned DGAT1 and DGAT2 genes of tung tree (Vernicia fordii), whose novel seed TAGs are useful in a wide range of industrial applications. The objective of this study was to understand the developmental regulation of DGAT family gene expression in tung tree. To this end, we first cloned a tung tree gene encoding DGAT3, a putatively soluble form of DGAT that possesses 11 completely conserved amino acid residues shared among 27 DGAT3s from 19 plant species. Unlike DGAT1 and DGAT2 subfamilies, DGAT3 is absent from animals. We then used TaqMan and SYBR Green quantitative real-time PCR, along with northern and western blotting, to study the expression patterns of the three DGAT genes in tung tree tissues. Expression results demonstrate that 1) all three isoforms of DGAT genes are expressed in developing seeds, leaves and flowers; 2) DGAT2 is the major DGAT mRNA in tung seeds, whose expression profile is well-coordinated with the oil profile in developing tung seeds; and 3) DGAT3 is the major form of DGAT mRNA in tung leaves, flowers and immature seeds prior to active tung oil biosynthesis. These results suggest that DGAT2 is probably the major TAG biosynthetic isoform in tung seeds and that DGAT3 gene likely plays a significant role in TAG metabolism in other tissues. Therefore, DGAT2 should be a primary target for tung oil engineering in transgenic organisms.Heping CaoJay M ShockeyK Thomas KlassonDorselyn C ChapitalCatherine B MasonBrian E SchefflerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e76946 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heping Cao Jay M Shockey K Thomas Klasson Dorselyn C Chapital Catherine B Mason Brian E Scheffler Developmental regulation of diacylglycerol acyltransferase family gene expression in tung tree tissues.
description	Diacylglycerol acyltransferases (DGAT) catalyze the final and rate-limiting step of triacylglycerol (TAG) biosynthesis in eukaryotic organisms. DGAT genes have been identified in numerous organisms. Multiple isoforms of DGAT are present in eukaryotes. We previously cloned DGAT1 and DGAT2 genes of tung tree (Vernicia fordii), whose novel seed TAGs are useful in a wide range of industrial applications. The objective of this study was to understand the developmental regulation of DGAT family gene expression in tung tree. To this end, we first cloned a tung tree gene encoding DGAT3, a putatively soluble form of DGAT that possesses 11 completely conserved amino acid residues shared among 27 DGAT3s from 19 plant species. Unlike DGAT1 and DGAT2 subfamilies, DGAT3 is absent from animals. We then used TaqMan and SYBR Green quantitative real-time PCR, along with northern and western blotting, to study the expression patterns of the three DGAT genes in tung tree tissues. Expression results demonstrate that 1) all three isoforms of DGAT genes are expressed in developing seeds, leaves and flowers; 2) DGAT2 is the major DGAT mRNA in tung seeds, whose expression profile is well-coordinated with the oil profile in developing tung seeds; and 3) DGAT3 is the major form of DGAT mRNA in tung leaves, flowers and immature seeds prior to active tung oil biosynthesis. These results suggest that DGAT2 is probably the major TAG biosynthetic isoform in tung seeds and that DGAT3 gene likely plays a significant role in TAG metabolism in other tissues. Therefore, DGAT2 should be a primary target for tung oil engineering in transgenic organisms.
format	article
author	Heping Cao Jay M Shockey K Thomas Klasson Dorselyn C Chapital Catherine B Mason Brian E Scheffler
author_facet	Heping Cao Jay M Shockey K Thomas Klasson Dorselyn C Chapital Catherine B Mason Brian E Scheffler
author_sort	Heping Cao
title	Developmental regulation of diacylglycerol acyltransferase family gene expression in tung tree tissues.
title_short	Developmental regulation of diacylglycerol acyltransferase family gene expression in tung tree tissues.
title_full	Developmental regulation of diacylglycerol acyltransferase family gene expression in tung tree tissues.
title_fullStr	Developmental regulation of diacylglycerol acyltransferase family gene expression in tung tree tissues.
title_full_unstemmed	Developmental regulation of diacylglycerol acyltransferase family gene expression in tung tree tissues.
title_sort	developmental regulation of diacylglycerol acyltransferase family gene expression in tung tree tissues.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/8e1143ac27e744dcb870ae6decef2ee0
work_keys_str_mv	AT hepingcao developmentalregulationofdiacylglycerolacyltransferasefamilygeneexpressionintungtreetissues AT jaymshockey developmentalregulationofdiacylglycerolacyltransferasefamilygeneexpressionintungtreetissues AT kthomasklasson developmentalregulationofdiacylglycerolacyltransferasefamilygeneexpressionintungtreetissues AT dorselyncchapital developmentalregulationofdiacylglycerolacyltransferasefamilygeneexpressionintungtreetissues AT catherinebmason developmentalregulationofdiacylglycerolacyltransferasefamilygeneexpressionintungtreetissues AT brianescheffler developmentalregulationofdiacylglycerolacyltransferasefamilygeneexpressionintungtreetissues
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Developmental regulation of diacylglycerol acyltransferase family gene expression in tung tree tissues.

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