Garcinol loaded vitamin E TPGS emulsified PLGA nanoparticles: preparation, physicochemical characterization, in vitro and in vivo studies

Abstract Garcinol (GAR) is a naturally occurring polyisoprenylated phenolic compound. It has been recently investigated for its biological activities such as antioxidant, anti-inflammatory, anti ulcer, and antiproliferative effect on a wide range of human cancer cell lines. Though the outcomes are v...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Raghuvir H. Gaonkar, Soumya Ganguly, Saikat Dewanjee, Samarendu Sinha, Amit Gupta, Shantanu Ganguly, Dipankar Chattopadhyay, Mita Chatterjee Debnath
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8e11acdf92bf440ea3afa4a579a61c7a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8e11acdf92bf440ea3afa4a579a61c7a
record_format	dspace
spelling	oai:doaj.org-article:8e11acdf92bf440ea3afa4a579a61c7a2021-12-02T12:32:16ZGarcinol loaded vitamin E TPGS emulsified PLGA nanoparticles: preparation, physicochemical characterization, in vitro and in vivo studies10.1038/s41598-017-00696-62045-2322https://doaj.org/article/8e11acdf92bf440ea3afa4a579a61c7a2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00696-6https://doaj.org/toc/2045-2322Abstract Garcinol (GAR) is a naturally occurring polyisoprenylated phenolic compound. It has been recently investigated for its biological activities such as antioxidant, anti-inflammatory, anti ulcer, and antiproliferative effect on a wide range of human cancer cell lines. Though the outcomes are very promising, its extreme insolubility in water remains the main obstacle for its clinical application. Herein we report the formulation of GAR entrapped PLGA nanoparticles by nanoprecipitation method using vitamin E TPGS as an emulsifier. The nanoparticles were characterized for size, surface morphology, surface charge, encapsulation efficiency and in vitro drug release kinetics. The MTT assay depicted a high amount of cytotoxicity of GAR-NPs in B16F10, HepG2 and KB cells. A considerable amount of cell apoptosis was observed in B16f10 and KB cell lines. In vivo cellular uptake of fluorescent NPs on B16F10 cells was also investigated. Finally the GAR loaded NPs were radiolabeled with technetium-99m with >95% labeling efficiency and administered to B16F10 melanoma tumor bearing mice to investigate the in vivo deposition at the tumor site by biodistribution and scintigraphic imaging study. In vitro cellular uptake studies and biological evaluation confirm the efficacy of the formulation for cancer treatment.Raghuvir H. GaonkarSoumya GangulySaikat DewanjeeSamarendu SinhaAmit GuptaShantanu GangulyDipankar ChattopadhyayMita Chatterjee DebnathNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Raghuvir H. Gaonkar Soumya Ganguly Saikat Dewanjee Samarendu Sinha Amit Gupta Shantanu Ganguly Dipankar Chattopadhyay Mita Chatterjee Debnath Garcinol loaded vitamin E TPGS emulsified PLGA nanoparticles: preparation, physicochemical characterization, in vitro and in vivo studies
description	Abstract Garcinol (GAR) is a naturally occurring polyisoprenylated phenolic compound. It has been recently investigated for its biological activities such as antioxidant, anti-inflammatory, anti ulcer, and antiproliferative effect on a wide range of human cancer cell lines. Though the outcomes are very promising, its extreme insolubility in water remains the main obstacle for its clinical application. Herein we report the formulation of GAR entrapped PLGA nanoparticles by nanoprecipitation method using vitamin E TPGS as an emulsifier. The nanoparticles were characterized for size, surface morphology, surface charge, encapsulation efficiency and in vitro drug release kinetics. The MTT assay depicted a high amount of cytotoxicity of GAR-NPs in B16F10, HepG2 and KB cells. A considerable amount of cell apoptosis was observed in B16f10 and KB cell lines. In vivo cellular uptake of fluorescent NPs on B16F10 cells was also investigated. Finally the GAR loaded NPs were radiolabeled with technetium-99m with >95% labeling efficiency and administered to B16F10 melanoma tumor bearing mice to investigate the in vivo deposition at the tumor site by biodistribution and scintigraphic imaging study. In vitro cellular uptake studies and biological evaluation confirm the efficacy of the formulation for cancer treatment.
format	article
author	Raghuvir H. Gaonkar Soumya Ganguly Saikat Dewanjee Samarendu Sinha Amit Gupta Shantanu Ganguly Dipankar Chattopadhyay Mita Chatterjee Debnath
author_facet	Raghuvir H. Gaonkar Soumya Ganguly Saikat Dewanjee Samarendu Sinha Amit Gupta Shantanu Ganguly Dipankar Chattopadhyay Mita Chatterjee Debnath
author_sort	Raghuvir H. Gaonkar
title	Garcinol loaded vitamin E TPGS emulsified PLGA nanoparticles: preparation, physicochemical characterization, in vitro and in vivo studies
title_short	Garcinol loaded vitamin E TPGS emulsified PLGA nanoparticles: preparation, physicochemical characterization, in vitro and in vivo studies
title_full	Garcinol loaded vitamin E TPGS emulsified PLGA nanoparticles: preparation, physicochemical characterization, in vitro and in vivo studies
title_fullStr	Garcinol loaded vitamin E TPGS emulsified PLGA nanoparticles: preparation, physicochemical characterization, in vitro and in vivo studies
title_full_unstemmed	Garcinol loaded vitamin E TPGS emulsified PLGA nanoparticles: preparation, physicochemical characterization, in vitro and in vivo studies
title_sort	garcinol loaded vitamin e tpgs emulsified plga nanoparticles: preparation, physicochemical characterization, in vitro and in vivo studies
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8e11acdf92bf440ea3afa4a579a61c7a
work_keys_str_mv	AT raghuvirhgaonkar garcinolloadedvitaminetpgsemulsifiedplgananoparticlespreparationphysicochemicalcharacterizationinvitroandinvivostudies AT soumyaganguly garcinolloadedvitaminetpgsemulsifiedplgananoparticlespreparationphysicochemicalcharacterizationinvitroandinvivostudies AT saikatdewanjee garcinolloadedvitaminetpgsemulsifiedplgananoparticlespreparationphysicochemicalcharacterizationinvitroandinvivostudies AT samarendusinha garcinolloadedvitaminetpgsemulsifiedplgananoparticlespreparationphysicochemicalcharacterizationinvitroandinvivostudies AT amitgupta garcinolloadedvitaminetpgsemulsifiedplgananoparticlespreparationphysicochemicalcharacterizationinvitroandinvivostudies AT shantanuganguly garcinolloadedvitaminetpgsemulsifiedplgananoparticlespreparationphysicochemicalcharacterizationinvitroandinvivostudies AT dipankarchattopadhyay garcinolloadedvitaminetpgsemulsifiedplgananoparticlespreparationphysicochemicalcharacterizationinvitroandinvivostudies AT mitachatterjeedebnath garcinolloadedvitaminetpgsemulsifiedplgananoparticlespreparationphysicochemicalcharacterizationinvitroandinvivostudies
_version_	1718394107028570112

Garcinol loaded vitamin E TPGS emulsified PLGA nanoparticles: preparation, physicochemical characterization, in vitro and in vivo studies

Ejemplares similares