Genistein diet improves body weight, serum glucose and triglyceride levels in both male and female ob/ob mice

Schuyler Rockwood,1 Daniel Mason,1 Ryan Lord,1 Peter Lamar,2 Walter Prozialeck,2 Layla Al-Nakkash3 1Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ, USA; 2Department of Pharmacology, College of Graduate Studies, Midwestern University, Downers Grove, IL, USA; 3Department...

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Autores principales:	Rockwood S, Mason D, Lord R, Lamar P, Prozialeck W, Al-Nakkash L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	genistein ob/ob diabetes phenotype Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/8e12d422e9434ebf9603592692667971
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spelling	oai:doaj.org-article:8e12d422e9434ebf96035926926679712021-12-02T05:44:33ZGenistein diet improves body weight, serum glucose and triglyceride levels in both male and female ob/ob mice1178-7007https://doaj.org/article/8e12d422e9434ebf96035926926679712019-10-01T00:00:00Zhttps://www.dovepress.com/genistein-diet-improves-body-weight-serum-glucose-and-triglyceride-lev-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Schuyler Rockwood,1 Daniel Mason,1 Ryan Lord,1 Peter Lamar,2 Walter Prozialeck,2 Layla Al-Nakkash3 1Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ, USA; 2Department of Pharmacology, College of Graduate Studies, Midwestern University, Downers Grove, IL, USA; 3Department of Physiology, College of Graduate Studies, Midwestern University, Glendale, AZ, USACorrespondence: Layla Al-NakkashDepartment of Physiology, College of Graduate Studies, Midwestern University, 19555 N. 59th Avenue, Glendale, AZ 85308, USATel +1 623 572 3719Fax +1 623 572 3673Email lalnak@midwestern.eduPurpose: Diabetic obesity in the leptin-deficient ob/ob mouse is associated with weight gain, and hyperglycemia, along with hyperinsulinemia. We have previously examined the effects of genistein (a naturally occurring isoflavone found in soy) on metabolic disturbances in the ob/ob mouse and demonstrated beneficial effects of genistein (600 mg genistein/kg diet, for 4-weeks) on T3 production and corticosterone status. The goal of this study was to examine whether dietary genistein could prevent, or at least lessen, the typical phenotype in this murine model of diabetic-obesity, and to assess potential sex-differences.Patients and methods: The ob/ob mice (male and female) aged 4–5 weeks were randomly assigned to one of two diets for a period of 4-weeks: standard rodent diet, or genistein-containing diet (600 mg genistein/kg diet). Comparisons were made to a lean control group.Results: Genistein diet significantly reduced body weight by 12% in females and 9% in males. Genistein significantly lowered serum glucose levels by 18% in females and 43% in males, yet had no effect on serum insulin. Genistein diet significantly lowered serum triglyceride levels in both ob/ob male and female mice returning them to lean levels. In females only, genistein significantly reduced serum pancreatic polypeptide levels by 56% and increased serum GIP levels 2.3-fold. Genistein had sex-dependent effects on hepatic steatosis: in females, genistein further increased the % fat area and the fat droplet diameter 2.6-fold, along with additionally increasing hepatic TBARS.Conclusion: The results from this study indicate interesting beneficial effects of genistein diet for both male and female ob/ob mice.Keywords: genistein, ob/ob, diabetes, phenotypeRockwood SMason DLord RLamar PProzialeck WAl-Nakkash LDove Medical Pressarticlegenisteinob/obdiabetesphenotypeSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 12, Pp 2011-2021 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	genistein ob/ob diabetes phenotype Specialties of internal medicine RC581-951
spellingShingle	genistein ob/ob diabetes phenotype Specialties of internal medicine RC581-951 Rockwood S Mason D Lord R Lamar P Prozialeck W Al-Nakkash L Genistein diet improves body weight, serum glucose and triglyceride levels in both male and female ob/ob mice
description	Schuyler Rockwood,1 Daniel Mason,1 Ryan Lord,1 Peter Lamar,2 Walter Prozialeck,2 Layla Al-Nakkash3 1Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ, USA; 2Department of Pharmacology, College of Graduate Studies, Midwestern University, Downers Grove, IL, USA; 3Department of Physiology, College of Graduate Studies, Midwestern University, Glendale, AZ, USACorrespondence: Layla Al-NakkashDepartment of Physiology, College of Graduate Studies, Midwestern University, 19555 N. 59th Avenue, Glendale, AZ 85308, USATel +1 623 572 3719Fax +1 623 572 3673Email lalnak@midwestern.eduPurpose: Diabetic obesity in the leptin-deficient ob/ob mouse is associated with weight gain, and hyperglycemia, along with hyperinsulinemia. We have previously examined the effects of genistein (a naturally occurring isoflavone found in soy) on metabolic disturbances in the ob/ob mouse and demonstrated beneficial effects of genistein (600 mg genistein/kg diet, for 4-weeks) on T3 production and corticosterone status. The goal of this study was to examine whether dietary genistein could prevent, or at least lessen, the typical phenotype in this murine model of diabetic-obesity, and to assess potential sex-differences.Patients and methods: The ob/ob mice (male and female) aged 4–5 weeks were randomly assigned to one of two diets for a period of 4-weeks: standard rodent diet, or genistein-containing diet (600 mg genistein/kg diet). Comparisons were made to a lean control group.Results: Genistein diet significantly reduced body weight by 12% in females and 9% in males. Genistein significantly lowered serum glucose levels by 18% in females and 43% in males, yet had no effect on serum insulin. Genistein diet significantly lowered serum triglyceride levels in both ob/ob male and female mice returning them to lean levels. In females only, genistein significantly reduced serum pancreatic polypeptide levels by 56% and increased serum GIP levels 2.3-fold. Genistein had sex-dependent effects on hepatic steatosis: in females, genistein further increased the % fat area and the fat droplet diameter 2.6-fold, along with additionally increasing hepatic TBARS.Conclusion: The results from this study indicate interesting beneficial effects of genistein diet for both male and female ob/ob mice.Keywords: genistein, ob/ob, diabetes, phenotype
format	article
author	Rockwood S Mason D Lord R Lamar P Prozialeck W Al-Nakkash L
author_facet	Rockwood S Mason D Lord R Lamar P Prozialeck W Al-Nakkash L
author_sort	Rockwood S
title	Genistein diet improves body weight, serum glucose and triglyceride levels in both male and female ob/ob mice
title_short	Genistein diet improves body weight, serum glucose and triglyceride levels in both male and female ob/ob mice
title_full	Genistein diet improves body weight, serum glucose and triglyceride levels in both male and female ob/ob mice
title_fullStr	Genistein diet improves body weight, serum glucose and triglyceride levels in both male and female ob/ob mice
title_full_unstemmed	Genistein diet improves body weight, serum glucose and triglyceride levels in both male and female ob/ob mice
title_sort	genistein diet improves body weight, serum glucose and triglyceride levels in both male and female ob/ob mice
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/8e12d422e9434ebf9603592692667971
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Genistein diet improves body weight, serum glucose and triglyceride levels in both male and female ob/ob mice

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