Comparative proteomics analysis of engineered Saccharomyces cerevisiae with enhanced biofuel precursor production.

The yeast Saccharomyces cerevisiae was metabolically modified for enhanced biofuel precursor production by knocking out genes encoding mitochondrial isocitrate dehydrogenase and over-expression of a heterologous ATP-citrate lyase. A comparative iTRAQ-coupled 2D LC-MS/MS analysis was performed to obt...

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Autores principales:	Xiaoling Tang, Huixing Feng, Jianhua Zhang, Wei Ning Chen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8e137766e78f4e81a505920bc67ed950
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spelling	oai:doaj.org-article:8e137766e78f4e81a505920bc67ed9502021-11-18T08:40:30ZComparative proteomics analysis of engineered Saccharomyces cerevisiae with enhanced biofuel precursor production.1932-620310.1371/journal.pone.0084661https://doaj.org/article/8e137766e78f4e81a505920bc67ed9502013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24376832/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The yeast Saccharomyces cerevisiae was metabolically modified for enhanced biofuel precursor production by knocking out genes encoding mitochondrial isocitrate dehydrogenase and over-expression of a heterologous ATP-citrate lyase. A comparative iTRAQ-coupled 2D LC-MS/MS analysis was performed to obtain a global overview of ubiquitous protein expression changes in S. cerevisiae engineered strains. More than 300 proteins were identified. Among these proteins, 37 were found differentially expressed in engineered strains and they were classified into specific categories based on their enzyme functions. Most of the proteins involved in glycolytic and pyruvate branch-point pathways were found to be up-regulated and the proteins involved in respiration and glyoxylate pathway were however found to be down-regulated in engineered strains. Moreover, the metabolic modification of S. cerevisiae cells resulted in a number of up-regulated proteins involved in stress response and differentially expressed proteins involved in amino acid metabolism and protein biosynthesis pathways. These LC-MS/MS based proteomics analysis results not only offered extensive information in identifying potential protein-protein interactions, signal pathways and ubiquitous cellular changes elicited by the engineered pathways, but also provided a meaningful biological information platform serving further modification of yeast cells for enhanced biofuel production.Xiaoling TangHuixing FengJianhua ZhangWei Ning ChenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e84661 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaoling Tang Huixing Feng Jianhua Zhang Wei Ning Chen Comparative proteomics analysis of engineered Saccharomyces cerevisiae with enhanced biofuel precursor production.
description	The yeast Saccharomyces cerevisiae was metabolically modified for enhanced biofuel precursor production by knocking out genes encoding mitochondrial isocitrate dehydrogenase and over-expression of a heterologous ATP-citrate lyase. A comparative iTRAQ-coupled 2D LC-MS/MS analysis was performed to obtain a global overview of ubiquitous protein expression changes in S. cerevisiae engineered strains. More than 300 proteins were identified. Among these proteins, 37 were found differentially expressed in engineered strains and they were classified into specific categories based on their enzyme functions. Most of the proteins involved in glycolytic and pyruvate branch-point pathways were found to be up-regulated and the proteins involved in respiration and glyoxylate pathway were however found to be down-regulated in engineered strains. Moreover, the metabolic modification of S. cerevisiae cells resulted in a number of up-regulated proteins involved in stress response and differentially expressed proteins involved in amino acid metabolism and protein biosynthesis pathways. These LC-MS/MS based proteomics analysis results not only offered extensive information in identifying potential protein-protein interactions, signal pathways and ubiquitous cellular changes elicited by the engineered pathways, but also provided a meaningful biological information platform serving further modification of yeast cells for enhanced biofuel production.
format	article
author	Xiaoling Tang Huixing Feng Jianhua Zhang Wei Ning Chen
author_facet	Xiaoling Tang Huixing Feng Jianhua Zhang Wei Ning Chen
author_sort	Xiaoling Tang
title	Comparative proteomics analysis of engineered Saccharomyces cerevisiae with enhanced biofuel precursor production.
title_short	Comparative proteomics analysis of engineered Saccharomyces cerevisiae with enhanced biofuel precursor production.
title_full	Comparative proteomics analysis of engineered Saccharomyces cerevisiae with enhanced biofuel precursor production.
title_fullStr	Comparative proteomics analysis of engineered Saccharomyces cerevisiae with enhanced biofuel precursor production.
title_full_unstemmed	Comparative proteomics analysis of engineered Saccharomyces cerevisiae with enhanced biofuel precursor production.
title_sort	comparative proteomics analysis of engineered saccharomyces cerevisiae with enhanced biofuel precursor production.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/8e137766e78f4e81a505920bc67ed950
work_keys_str_mv	AT xiaolingtang comparativeproteomicsanalysisofengineeredsaccharomycescerevisiaewithenhancedbiofuelprecursorproduction AT huixingfeng comparativeproteomicsanalysisofengineeredsaccharomycescerevisiaewithenhancedbiofuelprecursorproduction AT jianhuazhang comparativeproteomicsanalysisofengineeredsaccharomycescerevisiaewithenhancedbiofuelprecursorproduction AT weiningchen comparativeproteomicsanalysisofengineeredsaccharomycescerevisiaewithenhancedbiofuelprecursorproduction
_version_	1718421457866850304

Comparative proteomics analysis of engineered Saccharomyces cerevisiae with enhanced biofuel precursor production.

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