Deletion of the celA gene in Aspergillus nidulans triggers overexpression of secondary metabolite biosynthetic genes

Abstract Although much progress has been made in the study of cell wall biosynthetic genes in the model filamentous fungus Aspergillus nidulans, there are still targets awaiting characterization. An example is the gene celA (ANIA_08444) encoding a putative mixed linkage glucan synthase. To character...

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Autores principales:	Gea Guerriero, Lucia Silvestrini, Sylvain Legay, Frank Maixner, Michael Sulyok, Jean-Francois Hausman, Joseph Strauss
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8e1789917f0e439d9520f73af3d4bfb3
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spelling	oai:doaj.org-article:8e1789917f0e439d9520f73af3d4bfb32021-12-02T11:41:20ZDeletion of the celA gene in Aspergillus nidulans triggers overexpression of secondary metabolite biosynthetic genes10.1038/s41598-017-05920-x2045-2322https://doaj.org/article/8e1789917f0e439d9520f73af3d4bfb32017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05920-xhttps://doaj.org/toc/2045-2322Abstract Although much progress has been made in the study of cell wall biosynthetic genes in the model filamentous fungus Aspergillus nidulans, there are still targets awaiting characterization. An example is the gene celA (ANIA_08444) encoding a putative mixed linkage glucan synthase. To characterize the role of celA, we deleted it in A. nidulans, analyzed the phenotype of the mycelium and performed RNA-Seq. The strain shows a very strong phenotype, namely “balloons” along the hyphae and aberrant conidiophores, as well as an altered susceptibility to cell wall drugs. These data suggest a potential role of the gene in cell wall-related processes. The Gene Ontology term Enrichment analysis shows increased expression of secondary metabolite biosynthetic genes (sterigmatocystin in particular) in the deleted strain. Our results show that the deletion of celA triggers a strong phenotype reminiscent of cell wall-related aberrations and the upregulation of some secondary metabolite gene clusters in A. nidulans.Gea GuerrieroLucia SilvestriniSylvain LegayFrank MaixnerMichael SulyokJean-Francois HausmanJoseph StraussNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gea Guerriero Lucia Silvestrini Sylvain Legay Frank Maixner Michael Sulyok Jean-Francois Hausman Joseph Strauss Deletion of the celA gene in Aspergillus nidulans triggers overexpression of secondary metabolite biosynthetic genes
description	Abstract Although much progress has been made in the study of cell wall biosynthetic genes in the model filamentous fungus Aspergillus nidulans, there are still targets awaiting characterization. An example is the gene celA (ANIA_08444) encoding a putative mixed linkage glucan synthase. To characterize the role of celA, we deleted it in A. nidulans, analyzed the phenotype of the mycelium and performed RNA-Seq. The strain shows a very strong phenotype, namely “balloons” along the hyphae and aberrant conidiophores, as well as an altered susceptibility to cell wall drugs. These data suggest a potential role of the gene in cell wall-related processes. The Gene Ontology term Enrichment analysis shows increased expression of secondary metabolite biosynthetic genes (sterigmatocystin in particular) in the deleted strain. Our results show that the deletion of celA triggers a strong phenotype reminiscent of cell wall-related aberrations and the upregulation of some secondary metabolite gene clusters in A. nidulans.
format	article
author	Gea Guerriero Lucia Silvestrini Sylvain Legay Frank Maixner Michael Sulyok Jean-Francois Hausman Joseph Strauss
author_facet	Gea Guerriero Lucia Silvestrini Sylvain Legay Frank Maixner Michael Sulyok Jean-Francois Hausman Joseph Strauss
author_sort	Gea Guerriero
title	Deletion of the celA gene in Aspergillus nidulans triggers overexpression of secondary metabolite biosynthetic genes
title_short	Deletion of the celA gene in Aspergillus nidulans triggers overexpression of secondary metabolite biosynthetic genes
title_full	Deletion of the celA gene in Aspergillus nidulans triggers overexpression of secondary metabolite biosynthetic genes
title_fullStr	Deletion of the celA gene in Aspergillus nidulans triggers overexpression of secondary metabolite biosynthetic genes
title_full_unstemmed	Deletion of the celA gene in Aspergillus nidulans triggers overexpression of secondary metabolite biosynthetic genes
title_sort	deletion of the cela gene in aspergillus nidulans triggers overexpression of secondary metabolite biosynthetic genes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8e1789917f0e439d9520f73af3d4bfb3
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Deletion of the celA gene in Aspergillus nidulans triggers overexpression of secondary metabolite biosynthetic genes

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