Protective Roles of Folic Acid in the Responses of Bovine Mammary Epithelial Cells to Different Virulent Staphylococcus aureus Strains

Mastitis caused by Staphylococcus aureus (S. aureus) infection is one of the most difficult diseases to treat in dairy cattle. Exploring the biological progression of S. aureus mastitis via the interaction between host, pathogen, and environme...

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Autores principales:	Siyuan Mi, Yongjie Tang, Liangyu Shi, Xueqin Liu, Jingfang Si, Yuelin Yao, Serafino M. A. Augustino, Lingzhao Fang, Ying Yu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bovine mammary epithelial cells <i>Staphylococcus aureus</i> folic acid host and pathogen interactions RNA-seq DNA sensing pathway Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8e20b93c1dd24edcb109ed6654931925
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spelling	oai:doaj.org-article:8e20b93c1dd24edcb109ed66549319252021-11-25T16:47:36ZProtective Roles of Folic Acid in the Responses of Bovine Mammary Epithelial Cells to Different Virulent <i>Staphylococcus aureus</i> Strains10.3390/biology101111642079-7737https://doaj.org/article/8e20b93c1dd24edcb109ed66549319252021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1164https://doaj.org/toc/2079-7737Mastitis caused by <i>Staphylococcus aureus</i> (<i>S. aureus</i>) infection is one of the most difficult diseases to treat in dairy cattle. Exploring the biological progression of <i>S. aureus</i> mastitis via the interaction between host, pathogen, and environment is the key to an effective and sustainable improvement of animal health. Here, two strains of <i>S. aureus</i> and a strain of MRSA (Methicillin-resistant <i>Staphylococcus aureus</i>) isolated from cows with different inflammation phenotypes were used to challenge Mac-T cells and to investigate their effects on the global transcriptome of the cells, then to explore the potential regulatory mechanisms of folic acid on <i>S. aureus</i> mastitis prevention. Differential gene expression or splicing analysis showed that different strains of <i>S. aureus</i> led to distinct transcriptional responses from the host immune system. Folic acid could protect host defense against the challenge of <i>S. aureus</i> and MRSA partially through activating cytoplasmic DNA sensing and tight junction pathway. <i>ZBP1</i> at the upstream of cytoplasmic DNA sensing pathway was verified and related to anti-pathogen through RNA interference. Further enrichment analysis using these transcriptome data with cattle large-scale genome-wide association study (GWAS) data confirmed that <i>ZBP1</i> gene is highly associated with bovine somatic cell score (SCS) trait. Our data shed light on the potential effect of FA through regulating key gene and then protect host cells’ defense against <i>S. aureus</i> and MRSA.Siyuan MiYongjie TangLiangyu ShiXueqin LiuJingfang SiYuelin YaoSerafino M. A. AugustinoLingzhao FangYing YuMDPI AGarticlebovine mammary epithelial cells<i>Staphylococcus aureus</i>folic acidhost and pathogen interactionsRNA-seqDNA sensing pathwayBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1164, p 1164 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bovine mammary epithelial cells <i>Staphylococcus aureus</i> folic acid host and pathogen interactions RNA-seq DNA sensing pathway Biology (General) QH301-705.5
spellingShingle	bovine mammary epithelial cells <i>Staphylococcus aureus</i> folic acid host and pathogen interactions RNA-seq DNA sensing pathway Biology (General) QH301-705.5 Siyuan Mi Yongjie Tang Liangyu Shi Xueqin Liu Jingfang Si Yuelin Yao Serafino M. A. Augustino Lingzhao Fang Ying Yu Protective Roles of Folic Acid in the Responses of Bovine Mammary Epithelial Cells to Different Virulent <i>Staphylococcus aureus</i> Strains
description	Mastitis caused by <i>Staphylococcus aureus</i> (<i>S. aureus</i>) infection is one of the most difficult diseases to treat in dairy cattle. Exploring the biological progression of <i>S. aureus</i> mastitis via the interaction between host, pathogen, and environment is the key to an effective and sustainable improvement of animal health. Here, two strains of <i>S. aureus</i> and a strain of MRSA (Methicillin-resistant <i>Staphylococcus aureus</i>) isolated from cows with different inflammation phenotypes were used to challenge Mac-T cells and to investigate their effects on the global transcriptome of the cells, then to explore the potential regulatory mechanisms of folic acid on <i>S. aureus</i> mastitis prevention. Differential gene expression or splicing analysis showed that different strains of <i>S. aureus</i> led to distinct transcriptional responses from the host immune system. Folic acid could protect host defense against the challenge of <i>S. aureus</i> and MRSA partially through activating cytoplasmic DNA sensing and tight junction pathway. <i>ZBP1</i> at the upstream of cytoplasmic DNA sensing pathway was verified and related to anti-pathogen through RNA interference. Further enrichment analysis using these transcriptome data with cattle large-scale genome-wide association study (GWAS) data confirmed that <i>ZBP1</i> gene is highly associated with bovine somatic cell score (SCS) trait. Our data shed light on the potential effect of FA through regulating key gene and then protect host cells’ defense against <i>S. aureus</i> and MRSA.
format	article
author	Siyuan Mi Yongjie Tang Liangyu Shi Xueqin Liu Jingfang Si Yuelin Yao Serafino M. A. Augustino Lingzhao Fang Ying Yu
author_facet	Siyuan Mi Yongjie Tang Liangyu Shi Xueqin Liu Jingfang Si Yuelin Yao Serafino M. A. Augustino Lingzhao Fang Ying Yu
author_sort	Siyuan Mi
title	Protective Roles of Folic Acid in the Responses of Bovine Mammary Epithelial Cells to Different Virulent <i>Staphylococcus aureus</i> Strains
title_short	Protective Roles of Folic Acid in the Responses of Bovine Mammary Epithelial Cells to Different Virulent <i>Staphylococcus aureus</i> Strains
title_full	Protective Roles of Folic Acid in the Responses of Bovine Mammary Epithelial Cells to Different Virulent <i>Staphylococcus aureus</i> Strains
title_fullStr	Protective Roles of Folic Acid in the Responses of Bovine Mammary Epithelial Cells to Different Virulent <i>Staphylococcus aureus</i> Strains
title_full_unstemmed	Protective Roles of Folic Acid in the Responses of Bovine Mammary Epithelial Cells to Different Virulent <i>Staphylococcus aureus</i> Strains
title_sort	protective roles of folic acid in the responses of bovine mammary epithelial cells to different virulent <i>staphylococcus aureus</i> strains
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8e20b93c1dd24edcb109ed6654931925
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Protective Roles of Folic Acid in the Responses of Bovine Mammary Epithelial Cells to Different Virulent <i>Staphylococcus aureus</i> Strains

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