Prohibitins are required for cancer cell proliferation and adhesion.

Prohibitin 1 (PHB1) is a highly conserved protein that together with its homologue prohibitin 2 (PHB2) mainly localizes to the inner mitochondrial membrane. Although it was originally identified by its ability to inhibit G1/S progression in human fibroblasts, its role as tumor suppressor is debated....

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Autores principales:	Claudia Sievers, Gwendolyn Billig, Kathleen Gottschalk, Thomas Rudel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8e211fe834d7407d879d102cb32c8877
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spelling	oai:doaj.org-article:8e211fe834d7407d879d102cb32c88772021-11-18T06:35:12ZProhibitins are required for cancer cell proliferation and adhesion.1932-620310.1371/journal.pone.0012735https://doaj.org/article/8e211fe834d7407d879d102cb32c88772010-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20856874/?tool=EBIhttps://doaj.org/toc/1932-6203Prohibitin 1 (PHB1) is a highly conserved protein that together with its homologue prohibitin 2 (PHB2) mainly localizes to the inner mitochondrial membrane. Although it was originally identified by its ability to inhibit G1/S progression in human fibroblasts, its role as tumor suppressor is debated. To determine the function of prohibitins in maintaining cell homeostasis, we generated cancer cell lines expressing prohibitin-directed shRNAs. We show that prohibitin proteins are necessary for the proliferation of cancer cells. Down-regulation of prohibitin expression drastically reduced the rate of cell division. Furthermore, mitochondrial morphology was not affected, but loss of prohibitins did lead to the degradation of the fusion protein OPA1 and, in certain cancer cell lines, to a reduced capability to exhibit anchorage-independent growth. These cancer cells also exhibited reduced adhesion to the extracellular matrix. Taken together, these observations suggest prohibitins play a crucial role in adhesion processes in the cell and thereby sustaining cancer cell propagation and survival.Claudia SieversGwendolyn BilligKathleen GottschalkThomas RudelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 9, p e12735 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Claudia Sievers Gwendolyn Billig Kathleen Gottschalk Thomas Rudel Prohibitins are required for cancer cell proliferation and adhesion.
description	Prohibitin 1 (PHB1) is a highly conserved protein that together with its homologue prohibitin 2 (PHB2) mainly localizes to the inner mitochondrial membrane. Although it was originally identified by its ability to inhibit G1/S progression in human fibroblasts, its role as tumor suppressor is debated. To determine the function of prohibitins in maintaining cell homeostasis, we generated cancer cell lines expressing prohibitin-directed shRNAs. We show that prohibitin proteins are necessary for the proliferation of cancer cells. Down-regulation of prohibitin expression drastically reduced the rate of cell division. Furthermore, mitochondrial morphology was not affected, but loss of prohibitins did lead to the degradation of the fusion protein OPA1 and, in certain cancer cell lines, to a reduced capability to exhibit anchorage-independent growth. These cancer cells also exhibited reduced adhesion to the extracellular matrix. Taken together, these observations suggest prohibitins play a crucial role in adhesion processes in the cell and thereby sustaining cancer cell propagation and survival.
format	article
author	Claudia Sievers Gwendolyn Billig Kathleen Gottschalk Thomas Rudel
author_facet	Claudia Sievers Gwendolyn Billig Kathleen Gottschalk Thomas Rudel
author_sort	Claudia Sievers
title	Prohibitins are required for cancer cell proliferation and adhesion.
title_short	Prohibitins are required for cancer cell proliferation and adhesion.
title_full	Prohibitins are required for cancer cell proliferation and adhesion.
title_fullStr	Prohibitins are required for cancer cell proliferation and adhesion.
title_full_unstemmed	Prohibitins are required for cancer cell proliferation and adhesion.
title_sort	prohibitins are required for cancer cell proliferation and adhesion.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/8e211fe834d7407d879d102cb32c8877
work_keys_str_mv	AT claudiasievers prohibitinsarerequiredforcancercellproliferationandadhesion AT gwendolynbillig prohibitinsarerequiredforcancercellproliferationandadhesion AT kathleengottschalk prohibitinsarerequiredforcancercellproliferationandadhesion AT thomasrudel prohibitinsarerequiredforcancercellproliferationandadhesion
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Prohibitins are required for cancer cell proliferation and adhesion.

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