Antibacterial activity and cytotoxicity of multi-walled carbon nanotubes decorated with silver nanoparticles

Youngmin Seo,1 Jangsun Hwang,1 Jieun Kim,1 Yoon Jeong,1–3 Mintai P Hwang,3 Jonghoon Choi1,2 1Department of Bionano Technology, Graduate School, Hanyang University, Seoul, Korea; 2Department of Bionano Engineering, Hanyang University ERICA, Ansan, Korea; 3Center for Biomaterials, Korea In...

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Autores principales:	Seo Y, Hwang J, Kim J, Jeong Y, Hwang MP, Choi J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8e276c9b3c114b48a6430a9095fe8ff7
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spelling	oai:doaj.org-article:8e276c9b3c114b48a6430a9095fe8ff72021-12-02T01:49:54ZAntibacterial activity and cytotoxicity of multi-walled carbon nanotubes decorated with silver nanoparticles1178-2013https://doaj.org/article/8e276c9b3c114b48a6430a9095fe8ff72014-09-01T00:00:00Zhttp://www.dovepress.com/antibacterial-activity-and-cytotoxicity-of-multi-walled-carbon-nanotub-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Youngmin Seo,1 Jangsun Hwang,1 Jieun Kim,1 Yoon Jeong,1–3 Mintai P Hwang,3 Jonghoon Choi1,2 1Department of Bionano Technology, Graduate School, Hanyang University, Seoul, Korea; 2Department of Bionano Engineering, Hanyang University ERICA, Ansan, Korea; 3Center for Biomaterials, Korea Institute of Science and Technology, Seoul, Korea Abstract: Recently, various nanoscale materials, including silver (Ag) nanoparticles, have been actively studied for their capacity to effectively prevent bacterial growth. A critical challenge is to enhance the antibacterial properties of nanomaterials while maintaining their biocompatibility. The conjugation of multiple nanomaterials with different dimensions, such as spherical nanoparticles and high-aspect-ratio nanotubes, may increase the target-specific antibacterial capacity of the consequent nanostructure while retaining an optimal biocompatibility. In this study, multi-walled carbon nanotubes (MWCNTs) were treated with a mixture of acids and decorated with Ag nanoparticles via a chemical reduction of Ag cations by ethanol solution. The synthesized Ag-MWCNT complexes were characterized by transmission electron microscopy, X-ray diffractometry, and energy-dispersive X-ray spectroscopy. The antibacterial function of Ag-MWCNTs was evaluated against Methylobacterium spp. and Sphingomonas spp. In addition, the biocompatibility of Ag-MWCNTs was evaluated using both mouse liver hepatocytes (AML 12) and human peripheral blood mononuclear cells. Finally, we determined the minimum amount of Ag-MWCNTs required for a biocompatible yet effective antibacterial treatment modality. We report that 30 µg/mL of Ag-MWCNTs confers antibacterial functionality while maintaining minimal cytotoxicity toward both human and animal cells. The results reported herein would be beneficial for researchers interested in the efficient preparation of hybrid nanostructures and in determining the minimum amount of Ag-MWCNTs necessary to effectively hinder the growth of bacteria. Keywords: antimicrobial, nanoconstructs, toxicitySeo YHwang JKim JJeong YHwang MPChoi JDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 4621-4629 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Seo Y Hwang J Kim J Jeong Y Hwang MP Choi J Antibacterial activity and cytotoxicity of multi-walled carbon nanotubes decorated with silver nanoparticles
description	Youngmin Seo,1 Jangsun Hwang,1 Jieun Kim,1 Yoon Jeong,1–3 Mintai P Hwang,3 Jonghoon Choi1,2 1Department of Bionano Technology, Graduate School, Hanyang University, Seoul, Korea; 2Department of Bionano Engineering, Hanyang University ERICA, Ansan, Korea; 3Center for Biomaterials, Korea Institute of Science and Technology, Seoul, Korea Abstract: Recently, various nanoscale materials, including silver (Ag) nanoparticles, have been actively studied for their capacity to effectively prevent bacterial growth. A critical challenge is to enhance the antibacterial properties of nanomaterials while maintaining their biocompatibility. The conjugation of multiple nanomaterials with different dimensions, such as spherical nanoparticles and high-aspect-ratio nanotubes, may increase the target-specific antibacterial capacity of the consequent nanostructure while retaining an optimal biocompatibility. In this study, multi-walled carbon nanotubes (MWCNTs) were treated with a mixture of acids and decorated with Ag nanoparticles via a chemical reduction of Ag cations by ethanol solution. The synthesized Ag-MWCNT complexes were characterized by transmission electron microscopy, X-ray diffractometry, and energy-dispersive X-ray spectroscopy. The antibacterial function of Ag-MWCNTs was evaluated against Methylobacterium spp. and Sphingomonas spp. In addition, the biocompatibility of Ag-MWCNTs was evaluated using both mouse liver hepatocytes (AML 12) and human peripheral blood mononuclear cells. Finally, we determined the minimum amount of Ag-MWCNTs required for a biocompatible yet effective antibacterial treatment modality. We report that 30 µg/mL of Ag-MWCNTs confers antibacterial functionality while maintaining minimal cytotoxicity toward both human and animal cells. The results reported herein would be beneficial for researchers interested in the efficient preparation of hybrid nanostructures and in determining the minimum amount of Ag-MWCNTs necessary to effectively hinder the growth of bacteria. Keywords: antimicrobial, nanoconstructs, toxicity
format	article
author	Seo Y Hwang J Kim J Jeong Y Hwang MP Choi J
author_facet	Seo Y Hwang J Kim J Jeong Y Hwang MP Choi J
author_sort	Seo Y
title	Antibacterial activity and cytotoxicity of multi-walled carbon nanotubes decorated with silver nanoparticles
title_short	Antibacterial activity and cytotoxicity of multi-walled carbon nanotubes decorated with silver nanoparticles
title_full	Antibacterial activity and cytotoxicity of multi-walled carbon nanotubes decorated with silver nanoparticles
title_fullStr	Antibacterial activity and cytotoxicity of multi-walled carbon nanotubes decorated with silver nanoparticles
title_full_unstemmed	Antibacterial activity and cytotoxicity of multi-walled carbon nanotubes decorated with silver nanoparticles
title_sort	antibacterial activity and cytotoxicity of multi-walled carbon nanotubes decorated with silver nanoparticles
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/8e276c9b3c114b48a6430a9095fe8ff7
work_keys_str_mv	AT seoy antibacterialactivityandcytotoxicityofmultiwalledcarbonnanotubesdecoratedwithsilvernanoparticles AT hwangj antibacterialactivityandcytotoxicityofmultiwalledcarbonnanotubesdecoratedwithsilvernanoparticles AT kimj antibacterialactivityandcytotoxicityofmultiwalledcarbonnanotubesdecoratedwithsilvernanoparticles AT jeongy antibacterialactivityandcytotoxicityofmultiwalledcarbonnanotubesdecoratedwithsilvernanoparticles AT hwangmp antibacterialactivityandcytotoxicityofmultiwalledcarbonnanotubesdecoratedwithsilvernanoparticles AT choij antibacterialactivityandcytotoxicityofmultiwalledcarbonnanotubesdecoratedwithsilvernanoparticles
_version_	1718402853835374592

Antibacterial activity and cytotoxicity of multi-walled carbon nanotubes decorated with silver nanoparticles

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