Subcellular Localization of Connexin 26 in Cardiomyocytes and in Cardiomyocyte-Derived Extracellular Vesicles

Connexins (Cxs) are a family of membrane-spanning proteins, expressed in vertebrates and named according to their molecular weight. They are involved in tissue homeostasis, and they function by acting at several communication levels. Cardiac Cxs are responsible for regular heart function and, among...

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Autores principales:	Alessandra Falleni, Stefania Moscato, Antonietta R. M. Sabbatini, Margherita Bernardeschi, Francesco Bianchi, Antonella Cecchettini, Letizia Mattii
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	connexin26 immunoelectron microscopy H9c2 cells rat heart ultrastructure cardiomyocyte Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/8e2e190e7b2844a280ed34a7551c768d
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spelling	oai:doaj.org-article:8e2e190e7b2844a280ed34a7551c768d2021-11-11T18:39:51ZSubcellular Localization of Connexin 26 in Cardiomyocytes and in Cardiomyocyte-Derived Extracellular Vesicles10.3390/molecules262167261420-3049https://doaj.org/article/8e2e190e7b2844a280ed34a7551c768d2021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6726https://doaj.org/toc/1420-3049Connexins (Cxs) are a family of membrane-spanning proteins, expressed in vertebrates and named according to their molecular weight. They are involved in tissue homeostasis, and they function by acting at several communication levels. Cardiac Cxs are responsible for regular heart function and, among them, Cx26 and Cx43 are widely expressed throughout the heart. Cx26 is present in vessels, as well as in cardiomyocytes, and its localization is scattered all over the cell aside from at the intercalated discs as is the case for the other cardiac Cxs. However, having been found in cardiomyocytes only recently, both its subcellular localization and its functional characterization in cardiomyocytes remain poorly understood. Therefore, in this study we aimed to obtain further data on the localization of Cx26 at the subcellular level. Our TEM immunogold analyses were performed on rat heart ventricles and differentiated H9c2 cardiac cell sections as well as on differentiated H9c2 derived extracellular vesicles. The results confirmed the absence of Cx26 at intercalated discs and showed the presence of Cx26 at the level of different subcellular compartments. The peculiar localization at the level of extracellular vesicles suggested a specific role for cardiac Cx26 in inter-cellular communication in an independent gap junction manner.Alessandra FalleniStefania MoscatoAntonietta R. M. SabbatiniMargherita BernardeschiFrancesco BianchiAntonella CecchettiniLetizia MattiiMDPI AGarticleconnexin26immunoelectron microscopyH9c2 cellsrat heartultrastructurecardiomyocyteOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6726, p 6726 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	connexin26 immunoelectron microscopy H9c2 cells rat heart ultrastructure cardiomyocyte Organic chemistry QD241-441
spellingShingle	connexin26 immunoelectron microscopy H9c2 cells rat heart ultrastructure cardiomyocyte Organic chemistry QD241-441 Alessandra Falleni Stefania Moscato Antonietta R. M. Sabbatini Margherita Bernardeschi Francesco Bianchi Antonella Cecchettini Letizia Mattii Subcellular Localization of Connexin 26 in Cardiomyocytes and in Cardiomyocyte-Derived Extracellular Vesicles
description	Connexins (Cxs) are a family of membrane-spanning proteins, expressed in vertebrates and named according to their molecular weight. They are involved in tissue homeostasis, and they function by acting at several communication levels. Cardiac Cxs are responsible for regular heart function and, among them, Cx26 and Cx43 are widely expressed throughout the heart. Cx26 is present in vessels, as well as in cardiomyocytes, and its localization is scattered all over the cell aside from at the intercalated discs as is the case for the other cardiac Cxs. However, having been found in cardiomyocytes only recently, both its subcellular localization and its functional characterization in cardiomyocytes remain poorly understood. Therefore, in this study we aimed to obtain further data on the localization of Cx26 at the subcellular level. Our TEM immunogold analyses were performed on rat heart ventricles and differentiated H9c2 cardiac cell sections as well as on differentiated H9c2 derived extracellular vesicles. The results confirmed the absence of Cx26 at intercalated discs and showed the presence of Cx26 at the level of different subcellular compartments. The peculiar localization at the level of extracellular vesicles suggested a specific role for cardiac Cx26 in inter-cellular communication in an independent gap junction manner.
format	article
author	Alessandra Falleni Stefania Moscato Antonietta R. M. Sabbatini Margherita Bernardeschi Francesco Bianchi Antonella Cecchettini Letizia Mattii
author_facet	Alessandra Falleni Stefania Moscato Antonietta R. M. Sabbatini Margherita Bernardeschi Francesco Bianchi Antonella Cecchettini Letizia Mattii
author_sort	Alessandra Falleni
title	Subcellular Localization of Connexin 26 in Cardiomyocytes and in Cardiomyocyte-Derived Extracellular Vesicles
title_short	Subcellular Localization of Connexin 26 in Cardiomyocytes and in Cardiomyocyte-Derived Extracellular Vesicles
title_full	Subcellular Localization of Connexin 26 in Cardiomyocytes and in Cardiomyocyte-Derived Extracellular Vesicles
title_fullStr	Subcellular Localization of Connexin 26 in Cardiomyocytes and in Cardiomyocyte-Derived Extracellular Vesicles
title_full_unstemmed	Subcellular Localization of Connexin 26 in Cardiomyocytes and in Cardiomyocyte-Derived Extracellular Vesicles
title_sort	subcellular localization of connexin 26 in cardiomyocytes and in cardiomyocyte-derived extracellular vesicles
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8e2e190e7b2844a280ed34a7551c768d
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Subcellular Localization of Connexin 26 in Cardiomyocytes and in Cardiomyocyte-Derived Extracellular Vesicles

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