Influence of formic acid treatment on the proteome of the ectoparasite Varroa destructor.

The ectoparasite Varroa destructor Anderson and Trueman is the most important parasites of the western honey bee, Apis mellifera L. The most widely currently used treatment uses formic acid (FA), but the understanding of its effects on V. destructor is limited. In order to understand the mechanism o...

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Autores principales:	Antonia Genath, Hannes Petruschke, Martin von Bergen, Ralf Einspanier
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8e330002bb8045bf93c380508ce07e37
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spelling	oai:doaj.org-article:8e330002bb8045bf93c380508ce07e372021-12-02T20:16:34ZInfluence of formic acid treatment on the proteome of the ectoparasite Varroa destructor.1932-620310.1371/journal.pone.0258845https://doaj.org/article/8e330002bb8045bf93c380508ce07e372021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258845https://doaj.org/toc/1932-6203The ectoparasite Varroa destructor Anderson and Trueman is the most important parasites of the western honey bee, Apis mellifera L. The most widely currently used treatment uses formic acid (FA), but the understanding of its effects on V. destructor is limited. In order to understand the mechanism of action of FA, its effect on Varroa mites was investigated using proteomic analysis by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). V. destructor was collected from honey bee colonies with natural mite infestation before and 24 h after the initiation of FA treatment and subjected to proteome analysis. A total of 2637 proteins were identified. Quantitative analysis of differentially expressed candidate proteins (fold change ≥ 1.5; p ≤ 0.05) revealed 205 differentially expressed proteins: 91 were induced and 114 repressed in the FA-treated group compared to the untreated control group. Impaired protein synthesis accompanied by increased protein and amino acid degradation suggest an imbalance in proteostasis. Signs of oxidative stress included significant dysregulation of candidate proteins of mitochondrial cellular respiration, increased endocytosis, and induction of heat shock proteins. Furthermore, an increased concentration of several candidate proteins associated with detoxification was observed. These results suggest dysregulated cellular respiration triggered by FA treatment as well as an increase in cellular defense mechanisms, including induced heat shock proteins and detoxification enzymes.Antonia GenathHannes PetruschkeMartin von BergenRalf EinspanierPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0258845 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Antonia Genath Hannes Petruschke Martin von Bergen Ralf Einspanier Influence of formic acid treatment on the proteome of the ectoparasite Varroa destructor.
description	The ectoparasite Varroa destructor Anderson and Trueman is the most important parasites of the western honey bee, Apis mellifera L. The most widely currently used treatment uses formic acid (FA), but the understanding of its effects on V. destructor is limited. In order to understand the mechanism of action of FA, its effect on Varroa mites was investigated using proteomic analysis by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). V. destructor was collected from honey bee colonies with natural mite infestation before and 24 h after the initiation of FA treatment and subjected to proteome analysis. A total of 2637 proteins were identified. Quantitative analysis of differentially expressed candidate proteins (fold change ≥ 1.5; p ≤ 0.05) revealed 205 differentially expressed proteins: 91 were induced and 114 repressed in the FA-treated group compared to the untreated control group. Impaired protein synthesis accompanied by increased protein and amino acid degradation suggest an imbalance in proteostasis. Signs of oxidative stress included significant dysregulation of candidate proteins of mitochondrial cellular respiration, increased endocytosis, and induction of heat shock proteins. Furthermore, an increased concentration of several candidate proteins associated with detoxification was observed. These results suggest dysregulated cellular respiration triggered by FA treatment as well as an increase in cellular defense mechanisms, including induced heat shock proteins and detoxification enzymes.
format	article
author	Antonia Genath Hannes Petruschke Martin von Bergen Ralf Einspanier
author_facet	Antonia Genath Hannes Petruschke Martin von Bergen Ralf Einspanier
author_sort	Antonia Genath
title	Influence of formic acid treatment on the proteome of the ectoparasite Varroa destructor.
title_short	Influence of formic acid treatment on the proteome of the ectoparasite Varroa destructor.
title_full	Influence of formic acid treatment on the proteome of the ectoparasite Varroa destructor.
title_fullStr	Influence of formic acid treatment on the proteome of the ectoparasite Varroa destructor.
title_full_unstemmed	Influence of formic acid treatment on the proteome of the ectoparasite Varroa destructor.
title_sort	influence of formic acid treatment on the proteome of the ectoparasite varroa destructor.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/8e330002bb8045bf93c380508ce07e37
work_keys_str_mv	AT antoniagenath influenceofformicacidtreatmentontheproteomeoftheectoparasitevarroadestructor AT hannespetruschke influenceofformicacidtreatmentontheproteomeoftheectoparasitevarroadestructor AT martinvonbergen influenceofformicacidtreatmentontheproteomeoftheectoparasitevarroadestructor AT ralfeinspanier influenceofformicacidtreatmentontheproteomeoftheectoparasitevarroadestructor
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Influence of formic acid treatment on the proteome of the ectoparasite Varroa destructor.

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