Catalase biosensor based on the PAni/cMWCNT support for peroxide sensing

Polymeric-based composites can contribute to enhancing the detection, stability, and performance of enzymatic biosensors, due to their high structural stability, conductivity, and biocompatibility. This work presents the fabrication of a nanocomposite of polyaniline (PAni)/gold nanoparticles (AuNP)/...

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Autores principales:	Domínguez-Aragón Angélica, Dominguez Rocio B., Peralta-Pérez María del Rosario, Armando Zaragoza-Contreras Erasto
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	biosensor catalase hydrogen peroxide nanocomposite polyaniline Polymers and polymer manufacture TP1080-1185
Acceso en línea:	https://doaj.org/article/8e3649ed2bcd489e80fcfd4f72c9b05f
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spelling	oai:doaj.org-article:8e3649ed2bcd489e80fcfd4f72c9b05f2021-12-05T14:10:47ZCatalase biosensor based on the PAni/cMWCNT support for peroxide sensing1618-722910.1515/epoly-2021-0050https://doaj.org/article/8e3649ed2bcd489e80fcfd4f72c9b05f2021-07-01T00:00:00Zhttps://doi.org/10.1515/epoly-2021-0050https://doaj.org/toc/1618-7229Polymeric-based composites can contribute to enhancing the detection, stability, and performance of enzymatic biosensors, due to their high structural stability, conductivity, and biocompatibility. This work presents the fabrication of a nanocomposite of polyaniline (PAni)/gold nanoparticles (AuNP)/carboxylated multiwalled carbon nanotubes (cMWCNT) as functional support for covalently linked catalase (CAT) enzyme. PAni was electropolymerized on a screen-printed carbon electrode (SPCE) and decorated with AuNP to improve charge transfer properties. CAT was bonded through amide formation using the carboxylic groups of cMWCNT, resulting in PAni/AuNP/cMWCNT/CAT biosensor. The structural and electroactive characteristics of the nanocomposite were studied by SEM, FT-IR, and cyclic voltammetry. The optimal performance was achieved after CAT immobilization over PAni/AuNP/cMWCNT/nanocomposite, showing improved analytical features such as a fast amperometric response of 1.28 s, a wide detection range from 0.01 to 6.8 mM, a correlation coefficient (R 2) of 0.9921, a low detection limit of 2.34 µM, and an average recovery rate of 99.6% when evaluated in milk samples. Additionally, the bioelectrode showed excellent selectivity and retained bioactivity after 30 days of storage. Such remarkable performance proved the synergistic effects of both the high surface area of the cMWCNT and AuNP and the inherent PAni electroactivity, yielding direct electron transfer from CAT.Domínguez-Aragón AngélicaDominguez Rocio B.Peralta-Pérez María del RosarioArmando Zaragoza-Contreras ErastoDe Gruyterarticlebiosensorcatalasehydrogen peroxidenanocompositepolyanilinePolymers and polymer manufactureTP1080-1185ENe-Polymers, Vol 21, Iss 1, Pp 476-490 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biosensor catalase hydrogen peroxide nanocomposite polyaniline Polymers and polymer manufacture TP1080-1185
spellingShingle	biosensor catalase hydrogen peroxide nanocomposite polyaniline Polymers and polymer manufacture TP1080-1185 Domínguez-Aragón Angélica Dominguez Rocio B. Peralta-Pérez María del Rosario Armando Zaragoza-Contreras Erasto Catalase biosensor based on the PAni/cMWCNT support for peroxide sensing
description	Polymeric-based composites can contribute to enhancing the detection, stability, and performance of enzymatic biosensors, due to their high structural stability, conductivity, and biocompatibility. This work presents the fabrication of a nanocomposite of polyaniline (PAni)/gold nanoparticles (AuNP)/carboxylated multiwalled carbon nanotubes (cMWCNT) as functional support for covalently linked catalase (CAT) enzyme. PAni was electropolymerized on a screen-printed carbon electrode (SPCE) and decorated with AuNP to improve charge transfer properties. CAT was bonded through amide formation using the carboxylic groups of cMWCNT, resulting in PAni/AuNP/cMWCNT/CAT biosensor. The structural and electroactive characteristics of the nanocomposite were studied by SEM, FT-IR, and cyclic voltammetry. The optimal performance was achieved after CAT immobilization over PAni/AuNP/cMWCNT/nanocomposite, showing improved analytical features such as a fast amperometric response of 1.28 s, a wide detection range from 0.01 to 6.8 mM, a correlation coefficient (R 2) of 0.9921, a low detection limit of 2.34 µM, and an average recovery rate of 99.6% when evaluated in milk samples. Additionally, the bioelectrode showed excellent selectivity and retained bioactivity after 30 days of storage. Such remarkable performance proved the synergistic effects of both the high surface area of the cMWCNT and AuNP and the inherent PAni electroactivity, yielding direct electron transfer from CAT.
format	article
author	Domínguez-Aragón Angélica Dominguez Rocio B. Peralta-Pérez María del Rosario Armando Zaragoza-Contreras Erasto
author_facet	Domínguez-Aragón Angélica Dominguez Rocio B. Peralta-Pérez María del Rosario Armando Zaragoza-Contreras Erasto
author_sort	Domínguez-Aragón Angélica
title	Catalase biosensor based on the PAni/cMWCNT support for peroxide sensing
title_short	Catalase biosensor based on the PAni/cMWCNT support for peroxide sensing
title_full	Catalase biosensor based on the PAni/cMWCNT support for peroxide sensing
title_fullStr	Catalase biosensor based on the PAni/cMWCNT support for peroxide sensing
title_full_unstemmed	Catalase biosensor based on the PAni/cMWCNT support for peroxide sensing
title_sort	catalase biosensor based on the pani/cmwcnt support for peroxide sensing
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/8e3649ed2bcd489e80fcfd4f72c9b05f
work_keys_str_mv	AT dominguezaragonangelica catalasebiosensorbasedonthepanicmwcntsupportforperoxidesensing AT dominguezrociob catalasebiosensorbasedonthepanicmwcntsupportforperoxidesensing AT peraltaperezmariadelrosario catalasebiosensorbasedonthepanicmwcntsupportforperoxidesensing AT armandozaragozacontreraserasto catalasebiosensorbasedonthepanicmwcntsupportforperoxidesensing
_version_	1718371735386980352

Catalase biosensor based on the PAni/cMWCNT support for peroxide sensing

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