Adiponutrin and Adiponectin Gene Variants in Indonesian Patients with Non-Alcoholic Fatty Liver Disease: a Preliminary Study

Background Variants of adiponutrin (PNPLA3) and adiponectin (ADIPOQ) genes were considered to be associated with non-alcoholic fatty liver disease (NAFLD). Although the prevalence of NAFLD is increasing, there are limited numbers of studies about the association in Indonesian population. Objective...

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Autores principales:	Rayvita AN Meagratia, Ferdy Kurniawan Cayami, Udin Bahrudin, Wiwik Lestari, Nani Maharani, Sultana MH Faradz, Hery Djagat Purnomo
Formato:	article
Lenguaje:	EN
Publicado:	Diponegoro University 2021
Materias:	non-alcoholic fatty liver disease adiponectin adiponutrin indonesia Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8e3b97e014b14276b05b0d89fffa6488
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spelling	oai:doaj.org-article:8e3b97e014b14276b05b0d89fffa64882021-11-05T16:47:51ZAdiponutrin and Adiponectin Gene Variants in Indonesian Patients with Non-Alcoholic Fatty Liver Disease: a Preliminary Study2503-217810.14710/jbtr.v7i2.11777https://doaj.org/article/8e3b97e014b14276b05b0d89fffa64882021-08-01T00:00:00Zhttps://ejournal2.undip.ac.id/index.php/jbtr/article/view/11777https://doaj.org/toc/2503-2178Background Variants of adiponutrin (PNPLA3) and adiponectin (ADIPOQ) genes were considered to be associated with non-alcoholic fatty liver disease (NAFLD). Although the prevalence of NAFLD is increasing, there are limited numbers of studies about the association in Indonesian population. Objective To confirm whether specific variants of adiponutrin (PNPLA3) and adiponectin (ADIPOQ) genes are associated with NAFLD in Indonesian patients. Methods Data and DNA of 152 participants were obtained from a previous study in Dr. Kariadi Hospital, Semarang, Indonesia. PCR-RFLP analysis was performed for detection of PNPLA3 rs738409 and ADIPOQ rs2241767 variants. The diagnosis and severity of NAFLD were assessed according to NAFLD activity score (NAS) based on histopathology assessment of liverbiopsy. Results Allele G of PNPLA3rs738409 was associated with NAFLD in both bivariate (p=0.009, OR 2.52, CI 95% 1.25–5.07) and multivariate (p=0.008, OR 2.62, CI 95% 1.29%–5.32%) analysis, while ADIPOQ rs2241767 had no significant association. In NAFLD participants, both genotypes showed allele G was higher in the group of possible non-alcoholic steatohepatitis (NASH) – NASH (NAS >2) than in the simple steatosis group (NAS <2) i.e. 40.0% vs. 3.75% for the rs2241767 variant and 23.75% vs. 1.25% for the rs738409 variant, without significant association. Conclusion Variant PNPLA3 rs738409 was associated with NAFLD incidence in studied population. Among NAFLD participants, the frequency of both variants were found higher in the possible NASH – NASH group, yet needs to be confirmed with more participants and a multicenter study. Data and DNA of 152 participants were obtained from a previous study in Dr. Kariadi Hospital, Semarang, Indonesia. PCR-RFLP analysis was performed for detection of PNPLA3 rs738409 and ADIPOQ rs2241767 variants. The diagnosis and severity of NAFLD were assessed according to NAFLD activity score (NAS) based on histopathology assessment of liverbiopsy.Rayvita AN MeagratiaFerdy Kurniawan CayamiUdin BahrudinWiwik LestariNani MaharaniSultana MH FaradzHery Djagat PurnomoDiponegoro Universityarticlenon-alcoholic fatty liver diseaseadiponectinadiponutrinindonesiaMedicine (General)R5-920ENJournal of Biomedicine and Translational Research, Vol 7, Iss 2, Pp 86-91 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	non-alcoholic fatty liver disease adiponectin adiponutrin indonesia Medicine (General) R5-920
spellingShingle	non-alcoholic fatty liver disease adiponectin adiponutrin indonesia Medicine (General) R5-920 Rayvita AN Meagratia Ferdy Kurniawan Cayami Udin Bahrudin Wiwik Lestari Nani Maharani Sultana MH Faradz Hery Djagat Purnomo Adiponutrin and Adiponectin Gene Variants in Indonesian Patients with Non-Alcoholic Fatty Liver Disease: a Preliminary Study
description	Background Variants of adiponutrin (PNPLA3) and adiponectin (ADIPOQ) genes were considered to be associated with non-alcoholic fatty liver disease (NAFLD). Although the prevalence of NAFLD is increasing, there are limited numbers of studies about the association in Indonesian population. Objective To confirm whether specific variants of adiponutrin (PNPLA3) and adiponectin (ADIPOQ) genes are associated with NAFLD in Indonesian patients. Methods Data and DNA of 152 participants were obtained from a previous study in Dr. Kariadi Hospital, Semarang, Indonesia. PCR-RFLP analysis was performed for detection of PNPLA3 rs738409 and ADIPOQ rs2241767 variants. The diagnosis and severity of NAFLD were assessed according to NAFLD activity score (NAS) based on histopathology assessment of liverbiopsy. Results Allele G of PNPLA3rs738409 was associated with NAFLD in both bivariate (p=0.009, OR 2.52, CI 95% 1.25–5.07) and multivariate (p=0.008, OR 2.62, CI 95% 1.29%–5.32%) analysis, while ADIPOQ rs2241767 had no significant association. In NAFLD participants, both genotypes showed allele G was higher in the group of possible non-alcoholic steatohepatitis (NASH) – NASH (NAS >2) than in the simple steatosis group (NAS <2) i.e. 40.0% vs. 3.75% for the rs2241767 variant and 23.75% vs. 1.25% for the rs738409 variant, without significant association. Conclusion Variant PNPLA3 rs738409 was associated with NAFLD incidence in studied population. Among NAFLD participants, the frequency of both variants were found higher in the possible NASH – NASH group, yet needs to be confirmed with more participants and a multicenter study. Data and DNA of 152 participants were obtained from a previous study in Dr. Kariadi Hospital, Semarang, Indonesia. PCR-RFLP analysis was performed for detection of PNPLA3 rs738409 and ADIPOQ rs2241767 variants. The diagnosis and severity of NAFLD were assessed according to NAFLD activity score (NAS) based on histopathology assessment of liverbiopsy.
format	article
author	Rayvita AN Meagratia Ferdy Kurniawan Cayami Udin Bahrudin Wiwik Lestari Nani Maharani Sultana MH Faradz Hery Djagat Purnomo
author_facet	Rayvita AN Meagratia Ferdy Kurniawan Cayami Udin Bahrudin Wiwik Lestari Nani Maharani Sultana MH Faradz Hery Djagat Purnomo
author_sort	Rayvita AN Meagratia
title	Adiponutrin and Adiponectin Gene Variants in Indonesian Patients with Non-Alcoholic Fatty Liver Disease: a Preliminary Study
title_short	Adiponutrin and Adiponectin Gene Variants in Indonesian Patients with Non-Alcoholic Fatty Liver Disease: a Preliminary Study
title_full	Adiponutrin and Adiponectin Gene Variants in Indonesian Patients with Non-Alcoholic Fatty Liver Disease: a Preliminary Study
title_fullStr	Adiponutrin and Adiponectin Gene Variants in Indonesian Patients with Non-Alcoholic Fatty Liver Disease: a Preliminary Study
title_full_unstemmed	Adiponutrin and Adiponectin Gene Variants in Indonesian Patients with Non-Alcoholic Fatty Liver Disease: a Preliminary Study
title_sort	adiponutrin and adiponectin gene variants in indonesian patients with non-alcoholic fatty liver disease: a preliminary study
publisher	Diponegoro University
publishDate	2021
url	https://doaj.org/article/8e3b97e014b14276b05b0d89fffa6488
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Adiponutrin and Adiponectin Gene Variants in Indonesian Patients with Non-Alcoholic Fatty Liver Disease: a Preliminary Study

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