Amelioration of lithiatic injury to renal tissue by candesartan and sodium thiosulfate in a rat model of nephrolithiasis.

<h4>Aim</h4>Nephrolithiasis is a chronic metabolic condition affecting 10% of population worldwide. The present study aimed to investigate the possible protective role of candesartan (CAND) and sodium thiosulfate (STS) in ameliorating ethylene glycol (EG) induced nephrolithiasis.<h4&g...

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Autores principales:	Nahla E El-Ashmawy, Hoda A El-Bahrawy, Heba H Ashmawy, Eman G Khedr
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8e4e9c2a16c34d5d8f8a308db4a63ec4
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spelling	oai:doaj.org-article:8e4e9c2a16c34d5d8f8a308db4a63ec42021-12-02T20:04:03ZAmelioration of lithiatic injury to renal tissue by candesartan and sodium thiosulfate in a rat model of nephrolithiasis.1932-620310.1371/journal.pone.0251408https://doaj.org/article/8e4e9c2a16c34d5d8f8a308db4a63ec42021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251408https://doaj.org/toc/1932-6203<h4>Aim</h4>Nephrolithiasis is a chronic metabolic condition affecting 10% of population worldwide. The present study aimed to investigate the possible protective role of candesartan (CAND) and sodium thiosulfate (STS) in ameliorating ethylene glycol (EG) induced nephrolithiasis.<h4>Methods</h4>One hundred male Wistar rats were divided into five groups: Normal control group, nephrolithiasis (EG) group (1% EG in drinking water), Cystone (CYS) group (EG + 750 mg/kg CYS, orally, once daily), STS group (EG + 0.4 gm/kg STS, intraperitoneally, 3 times/week) and CAND group (EG + 70 μg/mL CAND in drinking water). Treatments and EG administration commenced on the same day and continued for 28 days. CYS was used as reference drug. Urine, blood, and renal tissues were collected at the end of the experiment for assessment of kidney function tests (serum creatinine and urea), urinary (8-hydroxydeoxyguanosine (8-OHdG), calcium and oxalate), inflammatory and oxdative stress biomarkers (transforming growth factor beta (TGF-β), osteopontin (OPN) and ratio of reduced glutathione to oxidized glutathione (GSH/GSSG)) in renal tissue.<h4>Results</h4>Serum (creatinine and urea), urinary (8-OHdG and oxalate) and renal (OPN and TGF-β) were significantly reduced in CAND and STS groups compared to EG group. Furthermore, renal GSH/GSSG and urinary calcium were significantly increased in CAND and STS groups compared to EG group. Histopathological results support the biochemical findings; CAND and STS groups showed less retention of crystals and necrotic damage in kidney. Also, microscopic examination of urine revealed less crystal for CAND and STS groups.<h4>Conclusion</h4>Candesartan and sodium thiosulfate exhibited protective effect against nephrolithiasis.Nahla E El-AshmawyHoda A El-BahrawyHeba H AshmawyEman G KhedrPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251408 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nahla E El-Ashmawy Hoda A El-Bahrawy Heba H Ashmawy Eman G Khedr Amelioration of lithiatic injury to renal tissue by candesartan and sodium thiosulfate in a rat model of nephrolithiasis.
description	<h4>Aim</h4>Nephrolithiasis is a chronic metabolic condition affecting 10% of population worldwide. The present study aimed to investigate the possible protective role of candesartan (CAND) and sodium thiosulfate (STS) in ameliorating ethylene glycol (EG) induced nephrolithiasis.<h4>Methods</h4>One hundred male Wistar rats were divided into five groups: Normal control group, nephrolithiasis (EG) group (1% EG in drinking water), Cystone (CYS) group (EG + 750 mg/kg CYS, orally, once daily), STS group (EG + 0.4 gm/kg STS, intraperitoneally, 3 times/week) and CAND group (EG + 70 μg/mL CAND in drinking water). Treatments and EG administration commenced on the same day and continued for 28 days. CYS was used as reference drug. Urine, blood, and renal tissues were collected at the end of the experiment for assessment of kidney function tests (serum creatinine and urea), urinary (8-hydroxydeoxyguanosine (8-OHdG), calcium and oxalate), inflammatory and oxdative stress biomarkers (transforming growth factor beta (TGF-β), osteopontin (OPN) and ratio of reduced glutathione to oxidized glutathione (GSH/GSSG)) in renal tissue.<h4>Results</h4>Serum (creatinine and urea), urinary (8-OHdG and oxalate) and renal (OPN and TGF-β) were significantly reduced in CAND and STS groups compared to EG group. Furthermore, renal GSH/GSSG and urinary calcium were significantly increased in CAND and STS groups compared to EG group. Histopathological results support the biochemical findings; CAND and STS groups showed less retention of crystals and necrotic damage in kidney. Also, microscopic examination of urine revealed less crystal for CAND and STS groups.<h4>Conclusion</h4>Candesartan and sodium thiosulfate exhibited protective effect against nephrolithiasis.
format	article
author	Nahla E El-Ashmawy Hoda A El-Bahrawy Heba H Ashmawy Eman G Khedr
author_facet	Nahla E El-Ashmawy Hoda A El-Bahrawy Heba H Ashmawy Eman G Khedr
author_sort	Nahla E El-Ashmawy
title	Amelioration of lithiatic injury to renal tissue by candesartan and sodium thiosulfate in a rat model of nephrolithiasis.
title_short	Amelioration of lithiatic injury to renal tissue by candesartan and sodium thiosulfate in a rat model of nephrolithiasis.
title_full	Amelioration of lithiatic injury to renal tissue by candesartan and sodium thiosulfate in a rat model of nephrolithiasis.
title_fullStr	Amelioration of lithiatic injury to renal tissue by candesartan and sodium thiosulfate in a rat model of nephrolithiasis.
title_full_unstemmed	Amelioration of lithiatic injury to renal tissue by candesartan and sodium thiosulfate in a rat model of nephrolithiasis.
title_sort	amelioration of lithiatic injury to renal tissue by candesartan and sodium thiosulfate in a rat model of nephrolithiasis.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/8e4e9c2a16c34d5d8f8a308db4a63ec4
work_keys_str_mv	AT nahlaeelashmawy ameliorationoflithiaticinjurytorenaltissuebycandesartanandsodiumthiosulfateinaratmodelofnephrolithiasis AT hodaaelbahrawy ameliorationoflithiaticinjurytorenaltissuebycandesartanandsodiumthiosulfateinaratmodelofnephrolithiasis AT hebahashmawy ameliorationoflithiaticinjurytorenaltissuebycandesartanandsodiumthiosulfateinaratmodelofnephrolithiasis AT emangkhedr ameliorationoflithiaticinjurytorenaltissuebycandesartanandsodiumthiosulfateinaratmodelofnephrolithiasis
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Amelioration of lithiatic injury to renal tissue by candesartan and sodium thiosulfate in a rat model of nephrolithiasis.

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