Gene expression profiling reveals new aspects of PIK3CA mutation in ERalpha-positive breast cancer: major implication of the Wnt signaling pathway.

<h4>Background</h4>The PI3K/AKT pathway plays a pivotal role in breast cancer development and maintenance. PIK3CA, encoding the PI3K catalytic subunit, is the oncogene exhibiting a high frequency of gain-of-function mutations leading to PI3K/AKT pathway activation in breast cancer. PIK3C...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Magdalena Cizkova, Géraldine Cizeron-Clairac, Sophie Vacher, Aurélie Susini, Catherine Andrieu, Rosette Lidereau, Ivan Bièche
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8e4f5fd6e0a24e7e86e2d9f1a968e445
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8e4f5fd6e0a24e7e86e2d9f1a968e445
record_format	dspace
spelling	oai:doaj.org-article:8e4f5fd6e0a24e7e86e2d9f1a968e4452021-11-18T07:00:56ZGene expression profiling reveals new aspects of PIK3CA mutation in ERalpha-positive breast cancer: major implication of the Wnt signaling pathway.1932-620310.1371/journal.pone.0015647https://doaj.org/article/8e4f5fd6e0a24e7e86e2d9f1a968e4452010-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21209903/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The PI3K/AKT pathway plays a pivotal role in breast cancer development and maintenance. PIK3CA, encoding the PI3K catalytic subunit, is the oncogene exhibiting a high frequency of gain-of-function mutations leading to PI3K/AKT pathway activation in breast cancer. PIK3CA mutations have been observed in 30% to 40% of ERα-positive breast tumors. However the physiopathological role of PIK3CA mutations in breast tumorigenesis remains largely unclear.<h4>Methodology/principal findings</h4>To identify relevant downstream target genes and signaling activated by aberrant PI3K/AKT pathway in breast tumors, we first analyzed gene expression with a pangenomic oligonucleotide microarray in a series of 43 ERα-positive tumors with and without PIK3CA mutations. Genes of interest were then investigated in 249 ERα-positive breast tumors by real-time quantitative RT-PCR. A robust collection of 19 genes was found to be differently expressed in PIK3CA-mutated tumors. PIK3CA mutations were associated with over-expression of several genes involved in the Wnt signaling pathway (WNT5A, TCF7L2, MSX2, TNFRSF11B), regulation of gene transcription (SEC14L2, MSX2, TFAP2B, NRIP3) and metal ion binding (CYP4Z1, CYP4Z2P, SLC40A1, LTF, LIMCH1).<h4>Conclusion/significance</h4>This new gene set should help to understand the behavior of PIK3CA-mutated cancers and detailed knowledge of Wnt signaling activation could lead to novel therapeutic strategies.Magdalena CizkovaGéraldine Cizeron-ClairacSophie VacherAurélie SusiniCatherine AndrieuRosette LidereauIvan BièchePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 12, p e15647 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Magdalena Cizkova Géraldine Cizeron-Clairac Sophie Vacher Aurélie Susini Catherine Andrieu Rosette Lidereau Ivan Bièche Gene expression profiling reveals new aspects of PIK3CA mutation in ERalpha-positive breast cancer: major implication of the Wnt signaling pathway.
description	<h4>Background</h4>The PI3K/AKT pathway plays a pivotal role in breast cancer development and maintenance. PIK3CA, encoding the PI3K catalytic subunit, is the oncogene exhibiting a high frequency of gain-of-function mutations leading to PI3K/AKT pathway activation in breast cancer. PIK3CA mutations have been observed in 30% to 40% of ERα-positive breast tumors. However the physiopathological role of PIK3CA mutations in breast tumorigenesis remains largely unclear.<h4>Methodology/principal findings</h4>To identify relevant downstream target genes and signaling activated by aberrant PI3K/AKT pathway in breast tumors, we first analyzed gene expression with a pangenomic oligonucleotide microarray in a series of 43 ERα-positive tumors with and without PIK3CA mutations. Genes of interest were then investigated in 249 ERα-positive breast tumors by real-time quantitative RT-PCR. A robust collection of 19 genes was found to be differently expressed in PIK3CA-mutated tumors. PIK3CA mutations were associated with over-expression of several genes involved in the Wnt signaling pathway (WNT5A, TCF7L2, MSX2, TNFRSF11B), regulation of gene transcription (SEC14L2, MSX2, TFAP2B, NRIP3) and metal ion binding (CYP4Z1, CYP4Z2P, SLC40A1, LTF, LIMCH1).<h4>Conclusion/significance</h4>This new gene set should help to understand the behavior of PIK3CA-mutated cancers and detailed knowledge of Wnt signaling activation could lead to novel therapeutic strategies.
format	article
author	Magdalena Cizkova Géraldine Cizeron-Clairac Sophie Vacher Aurélie Susini Catherine Andrieu Rosette Lidereau Ivan Bièche
author_facet	Magdalena Cizkova Géraldine Cizeron-Clairac Sophie Vacher Aurélie Susini Catherine Andrieu Rosette Lidereau Ivan Bièche
author_sort	Magdalena Cizkova
title	Gene expression profiling reveals new aspects of PIK3CA mutation in ERalpha-positive breast cancer: major implication of the Wnt signaling pathway.
title_short	Gene expression profiling reveals new aspects of PIK3CA mutation in ERalpha-positive breast cancer: major implication of the Wnt signaling pathway.
title_full	Gene expression profiling reveals new aspects of PIK3CA mutation in ERalpha-positive breast cancer: major implication of the Wnt signaling pathway.
title_fullStr	Gene expression profiling reveals new aspects of PIK3CA mutation in ERalpha-positive breast cancer: major implication of the Wnt signaling pathway.
title_full_unstemmed	Gene expression profiling reveals new aspects of PIK3CA mutation in ERalpha-positive breast cancer: major implication of the Wnt signaling pathway.
title_sort	gene expression profiling reveals new aspects of pik3ca mutation in eralpha-positive breast cancer: major implication of the wnt signaling pathway.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/8e4f5fd6e0a24e7e86e2d9f1a968e445
work_keys_str_mv	AT magdalenacizkova geneexpressionprofilingrevealsnewaspectsofpik3camutationineralphapositivebreastcancermajorimplicationofthewntsignalingpathway AT geraldinecizeronclairac geneexpressionprofilingrevealsnewaspectsofpik3camutationineralphapositivebreastcancermajorimplicationofthewntsignalingpathway AT sophievacher geneexpressionprofilingrevealsnewaspectsofpik3camutationineralphapositivebreastcancermajorimplicationofthewntsignalingpathway AT aureliesusini geneexpressionprofilingrevealsnewaspectsofpik3camutationineralphapositivebreastcancermajorimplicationofthewntsignalingpathway AT catherineandrieu geneexpressionprofilingrevealsnewaspectsofpik3camutationineralphapositivebreastcancermajorimplicationofthewntsignalingpathway AT rosettelidereau geneexpressionprofilingrevealsnewaspectsofpik3camutationineralphapositivebreastcancermajorimplicationofthewntsignalingpathway AT ivanbieche geneexpressionprofilingrevealsnewaspectsofpik3camutationineralphapositivebreastcancermajorimplicationofthewntsignalingpathway
_version_	1718424056163729408

Gene expression profiling reveals new aspects of PIK3CA mutation in ERalpha-positive breast cancer: major implication of the Wnt signaling pathway.

Ejemplares similares