In vitro effects of nonesterified fatty acids and β-hydroxybutyric acid on inflammatory cytokine expression in bovine peripheral blood leukocytes

Negative energy balance (NEB) occurs in dairy cows during the transition period, during which time the blood concentrations of nonesterified fatty acids (NEFA) and metabolic ketones such as β-hydroxybutyric acid (BHBA) are elevated. These increased levels may disrupt immune functions and are regarde...

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Autores principales:	Cheng-Yan Li, Yu-Wen Liao, Chia-So Liu, Chuen-Yu Cheng, Jacky Peng-Wen Chan, Chien-Kai Wang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	negative energy balance nonesterified fatty acids β-hydroxybutyric acid bovine peripheral blood leukocytes inflammatory cytokines Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/8e54bbf8f85b4389b3f6b4c6e0113084
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spelling	oai:doaj.org-article:8e54bbf8f85b4389b3f6b4c6e01130842021-12-01T14:40:59ZIn vitro effects of nonesterified fatty acids and β-hydroxybutyric acid on inflammatory cytokine expression in bovine peripheral blood leukocytes1594-40771828-051X10.1080/1828051X.2021.2006806https://doaj.org/article/8e54bbf8f85b4389b3f6b4c6e01130842021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/1828051X.2021.2006806https://doaj.org/toc/1594-4077https://doaj.org/toc/1828-051XNegative energy balance (NEB) occurs in dairy cows during the transition period, during which time the blood concentrations of nonesterified fatty acids (NEFA) and metabolic ketones such as β-hydroxybutyric acid (BHBA) are elevated. These increased levels may disrupt immune functions and are regarded as risk factors associated with postparturient inflammatory disorders. Therefore, the aim of this study was to investigate the effects of NEFAs and BHBA on inflammatory cytokine expression in bovine peripheral leukocytes in vitro. Peripheral blood mononuclear cells (PBMCs) and peripheral polymorphonuclear leukocytes (PMNLs) were collected from 16 Holstein cows and treated for 4 h with NEFA (0.1, 0.6, and 1.5 mM) or BHBA (5 and 10 mM), alone or combination with lipopolysaccharides (LPS, 1 µg/mL). The expression levels of the inflammatory cytokines TNF-α, IL-1β, IL-6, IL-8, and IL-10 were then determined. The results indicated that PBMCs and PMNLs responded to NEFA but tolerated BHBA. NEFA treatment dose-dependently induced the expression of TNF-α, IL-1β, IL-8, and IL-10 in PBMCs and that of IL-1β, IL-6, IL-8, and IL-10 in PMNLs. Combination treatment of LPS with NEFA further increased the levels of IL-10 expression in PBMCs and IL-1β and IL-6 expression in PMNLs. Taken together, these findings suggest that NEFA and BHBA enhance the expression of inflammatory cytokines in PBMCs and PMNLs and may disrupt the immune regulation of peripheral leukocytes, leading to an increased risk of inflammatory disorders. Preventing increases in blood NEFA concentration may thus help to reduce the risk of inflammatory disorders in dairy cows during the transition period.Highlights In cows with NEB, during the transition period, NEFA but not BHBA induced proinflammatory cytokine expression in PBMCs and PMNLs. NEFA enhanced LPS-induced proinflammatory cytokine expression in both PBMCs and PMNLs.Cheng-Yan LiYu-Wen LiaoChia-So LiuChuen-Yu ChengJacky Peng-Wen ChanChien-Kai WangTaylor & Francis Grouparticlenegative energy balancenonesterified fatty acidsβ-hydroxybutyric acidbovine peripheral blood leukocytesinflammatory cytokinesAnimal cultureSF1-1100ENItalian Journal of Animal Science, Vol 20, Iss 1, Pp 2197-2210 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	negative energy balance nonesterified fatty acids β-hydroxybutyric acid bovine peripheral blood leukocytes inflammatory cytokines Animal culture SF1-1100
spellingShingle	negative energy balance nonesterified fatty acids β-hydroxybutyric acid bovine peripheral blood leukocytes inflammatory cytokines Animal culture SF1-1100 Cheng-Yan Li Yu-Wen Liao Chia-So Liu Chuen-Yu Cheng Jacky Peng-Wen Chan Chien-Kai Wang In vitro effects of nonesterified fatty acids and β-hydroxybutyric acid on inflammatory cytokine expression in bovine peripheral blood leukocytes
description	Negative energy balance (NEB) occurs in dairy cows during the transition period, during which time the blood concentrations of nonesterified fatty acids (NEFA) and metabolic ketones such as β-hydroxybutyric acid (BHBA) are elevated. These increased levels may disrupt immune functions and are regarded as risk factors associated with postparturient inflammatory disorders. Therefore, the aim of this study was to investigate the effects of NEFAs and BHBA on inflammatory cytokine expression in bovine peripheral leukocytes in vitro. Peripheral blood mononuclear cells (PBMCs) and peripheral polymorphonuclear leukocytes (PMNLs) were collected from 16 Holstein cows and treated for 4 h with NEFA (0.1, 0.6, and 1.5 mM) or BHBA (5 and 10 mM), alone or combination with lipopolysaccharides (LPS, 1 µg/mL). The expression levels of the inflammatory cytokines TNF-α, IL-1β, IL-6, IL-8, and IL-10 were then determined. The results indicated that PBMCs and PMNLs responded to NEFA but tolerated BHBA. NEFA treatment dose-dependently induced the expression of TNF-α, IL-1β, IL-8, and IL-10 in PBMCs and that of IL-1β, IL-6, IL-8, and IL-10 in PMNLs. Combination treatment of LPS with NEFA further increased the levels of IL-10 expression in PBMCs and IL-1β and IL-6 expression in PMNLs. Taken together, these findings suggest that NEFA and BHBA enhance the expression of inflammatory cytokines in PBMCs and PMNLs and may disrupt the immune regulation of peripheral leukocytes, leading to an increased risk of inflammatory disorders. Preventing increases in blood NEFA concentration may thus help to reduce the risk of inflammatory disorders in dairy cows during the transition period.Highlights In cows with NEB, during the transition period, NEFA but not BHBA induced proinflammatory cytokine expression in PBMCs and PMNLs. NEFA enhanced LPS-induced proinflammatory cytokine expression in both PBMCs and PMNLs.
format	article
author	Cheng-Yan Li Yu-Wen Liao Chia-So Liu Chuen-Yu Cheng Jacky Peng-Wen Chan Chien-Kai Wang
author_facet	Cheng-Yan Li Yu-Wen Liao Chia-So Liu Chuen-Yu Cheng Jacky Peng-Wen Chan Chien-Kai Wang
author_sort	Cheng-Yan Li
title	In vitro effects of nonesterified fatty acids and β-hydroxybutyric acid on inflammatory cytokine expression in bovine peripheral blood leukocytes
title_short	In vitro effects of nonesterified fatty acids and β-hydroxybutyric acid on inflammatory cytokine expression in bovine peripheral blood leukocytes
title_full	In vitro effects of nonesterified fatty acids and β-hydroxybutyric acid on inflammatory cytokine expression in bovine peripheral blood leukocytes
title_fullStr	In vitro effects of nonesterified fatty acids and β-hydroxybutyric acid on inflammatory cytokine expression in bovine peripheral blood leukocytes
title_full_unstemmed	In vitro effects of nonesterified fatty acids and β-hydroxybutyric acid on inflammatory cytokine expression in bovine peripheral blood leukocytes
title_sort	in vitro effects of nonesterified fatty acids and β-hydroxybutyric acid on inflammatory cytokine expression in bovine peripheral blood leukocytes
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/8e54bbf8f85b4389b3f6b4c6e0113084
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In vitro effects of nonesterified fatty acids and β-hydroxybutyric acid on inflammatory cytokine expression in bovine peripheral blood leukocytes

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