Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR46 azole resistance gene

Abstract Infections due to triazole-resistant Aspergillus fumigatus are increasingly reported worldwide and are associated with treatment failure and mortality. The principal class of azole-resistant isolates is characterized by tandem repeats of 34 bp or 46 bp within the promoter region of the cyp5...

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Autores principales:	Plinio Trabasso, Tetsuhiro Matsuzawa, Teppei Arai, Daisuke Hagiwara, Yuzuru Mikami, Maria Luiza Moretti, Akira Watanabe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8e56991fa5f449ef83483180f4e821fb
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spelling	oai:doaj.org-article:8e56991fa5f449ef83483180f4e821fb2021-12-02T18:53:19ZDevelopment and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR46 azole resistance gene10.1038/s41598-021-96651-72045-2322https://doaj.org/article/8e56991fa5f449ef83483180f4e821fb2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96651-7https://doaj.org/toc/2045-2322Abstract Infections due to triazole-resistant Aspergillus fumigatus are increasingly reported worldwide and are associated with treatment failure and mortality. The principal class of azole-resistant isolates is characterized by tandem repeats of 34 bp or 46 bp within the promoter region of the cyp51A gene. Loop-mediated isothermal amplification (LAMP) is a widely used nucleic acid amplification system that is fast and specific. Here we describe a LAMP assay method to detect the 46 bp tandem repeat insertion in the cyp51A gene promoter region based on novel LAMP primer sets. It also differentiated strains with TR46 tandem repeats from those with TR34 tandem repeats. These results showed this TR46-LAMP method is specific, rapid, and provides crucial insights to develop novel antifungal therapeutic strategies against severe fungal infections due to A. fumigatus with TR46 tandem repeats.Plinio TrabassoTetsuhiro MatsuzawaTeppei AraiDaisuke HagiwaraYuzuru MikamiMaria Luiza MorettiAkira WatanabeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Plinio Trabasso Tetsuhiro Matsuzawa Teppei Arai Daisuke Hagiwara Yuzuru Mikami Maria Luiza Moretti Akira Watanabe Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR46 azole resistance gene
description	Abstract Infections due to triazole-resistant Aspergillus fumigatus are increasingly reported worldwide and are associated with treatment failure and mortality. The principal class of azole-resistant isolates is characterized by tandem repeats of 34 bp or 46 bp within the promoter region of the cyp51A gene. Loop-mediated isothermal amplification (LAMP) is a widely used nucleic acid amplification system that is fast and specific. Here we describe a LAMP assay method to detect the 46 bp tandem repeat insertion in the cyp51A gene promoter region based on novel LAMP primer sets. It also differentiated strains with TR46 tandem repeats from those with TR34 tandem repeats. These results showed this TR46-LAMP method is specific, rapid, and provides crucial insights to develop novel antifungal therapeutic strategies against severe fungal infections due to A. fumigatus with TR46 tandem repeats.
format	article
author	Plinio Trabasso Tetsuhiro Matsuzawa Teppei Arai Daisuke Hagiwara Yuzuru Mikami Maria Luiza Moretti Akira Watanabe
author_facet	Plinio Trabasso Tetsuhiro Matsuzawa Teppei Arai Daisuke Hagiwara Yuzuru Mikami Maria Luiza Moretti Akira Watanabe
author_sort	Plinio Trabasso
title	Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR46 azole resistance gene
title_short	Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR46 azole resistance gene
title_full	Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR46 azole resistance gene
title_fullStr	Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR46 azole resistance gene
title_full_unstemmed	Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR46 azole resistance gene
title_sort	development and validation of lamp primer sets for rapid identification of aspergillus fumigatus carrying the cyp51a tr46 azole resistance gene
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8e56991fa5f449ef83483180f4e821fb
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Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR46 azole resistance gene

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