Acoustic focusing of beads and cells in hydrogel droplets

Abstract The generation of hydrogel droplets using droplet microfluidics has emerged as a powerful tool with many applications in biology and medicine. Here, a microfluidic system to control the position of particles (beads or astrocyte cells) in hydrogel droplets using bulk acoustic standing waves...

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Autores principales:	Anna Fornell, Hannah Pohlit, Qian Shi, Maria Tenje
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8e599fd24700444baa0c659e36bb43f6
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spelling	oai:doaj.org-article:8e599fd24700444baa0c659e36bb43f62021-12-02T18:15:24ZAcoustic focusing of beads and cells in hydrogel droplets10.1038/s41598-021-86985-72045-2322https://doaj.org/article/8e599fd24700444baa0c659e36bb43f62021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86985-7https://doaj.org/toc/2045-2322Abstract The generation of hydrogel droplets using droplet microfluidics has emerged as a powerful tool with many applications in biology and medicine. Here, a microfluidic system to control the position of particles (beads or astrocyte cells) in hydrogel droplets using bulk acoustic standing waves is presented. The chip consisted of a droplet generator and a 380 µm wide acoustic focusing channel. Droplets comprising hydrogel precursor solution (polyethylene glycol tetraacrylate or a combination of polyethylene glycol tetraacrylate and gelatine methacrylate), photoinitiator and particles were generated. The droplets passed along the acoustic focusing channel where a half wavelength acoustic standing wave field was generated, and the particles were focused to the centre line of the droplets (i.e. the pressure nodal line) by the acoustic force. The droplets were cross-linked by exposure to UV-light, freezing the particles in their positions. With the acoustics applied, 89 ± 19% of the particles (polystyrene beads, 10 µm diameter) were positioned in an area ± 10% from the centre line. As proof-of-principle for biological particles, astrocytes were focused in hydrogel droplets using the same principle. The viability of the astrocytes after 7 days in culture was 72 ± 22% when exposed to the acoustic focusing compared with 70 ± 19% for samples not exposed to the acoustic focusing. This technology provides a platform to control the spatial position of bioparticles in hydrogel droplets, and opens up for the generation of more complex biological hydrogel structures.Anna FornellHannah PohlitQian ShiMaria TenjeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna Fornell Hannah Pohlit Qian Shi Maria Tenje Acoustic focusing of beads and cells in hydrogel droplets
description	Abstract The generation of hydrogel droplets using droplet microfluidics has emerged as a powerful tool with many applications in biology and medicine. Here, a microfluidic system to control the position of particles (beads or astrocyte cells) in hydrogel droplets using bulk acoustic standing waves is presented. The chip consisted of a droplet generator and a 380 µm wide acoustic focusing channel. Droplets comprising hydrogel precursor solution (polyethylene glycol tetraacrylate or a combination of polyethylene glycol tetraacrylate and gelatine methacrylate), photoinitiator and particles were generated. The droplets passed along the acoustic focusing channel where a half wavelength acoustic standing wave field was generated, and the particles were focused to the centre line of the droplets (i.e. the pressure nodal line) by the acoustic force. The droplets were cross-linked by exposure to UV-light, freezing the particles in their positions. With the acoustics applied, 89 ± 19% of the particles (polystyrene beads, 10 µm diameter) were positioned in an area ± 10% from the centre line. As proof-of-principle for biological particles, astrocytes were focused in hydrogel droplets using the same principle. The viability of the astrocytes after 7 days in culture was 72 ± 22% when exposed to the acoustic focusing compared with 70 ± 19% for samples not exposed to the acoustic focusing. This technology provides a platform to control the spatial position of bioparticles in hydrogel droplets, and opens up for the generation of more complex biological hydrogel structures.
format	article
author	Anna Fornell Hannah Pohlit Qian Shi Maria Tenje
author_facet	Anna Fornell Hannah Pohlit Qian Shi Maria Tenje
author_sort	Anna Fornell
title	Acoustic focusing of beads and cells in hydrogel droplets
title_short	Acoustic focusing of beads and cells in hydrogel droplets
title_full	Acoustic focusing of beads and cells in hydrogel droplets
title_fullStr	Acoustic focusing of beads and cells in hydrogel droplets
title_full_unstemmed	Acoustic focusing of beads and cells in hydrogel droplets
title_sort	acoustic focusing of beads and cells in hydrogel droplets
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8e599fd24700444baa0c659e36bb43f6
work_keys_str_mv	AT annafornell acousticfocusingofbeadsandcellsinhydrogeldroplets AT hannahpohlit acousticfocusingofbeadsandcellsinhydrogeldroplets AT qianshi acousticfocusingofbeadsandcellsinhydrogeldroplets AT mariatenje acousticfocusingofbeadsandcellsinhydrogeldroplets
_version_	1718378406954926080

Acoustic focusing of beads and cells in hydrogel droplets

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