Distinct Gut Microbiota Induced by Different Fat-to-Sugar-Ratio High-Energy Diets Share Similar Pro-obesity Genetic and Metabolite Profiles in Prediabetic Mice

ABSTRACT Gut microbiota play important roles in host metabolism, especially in diabetes. However, why different diets lead to similar diabetic states despite being associated with different microbiota is not clear. Mice were fed two high-energy diets (HED) with the same energy density but different...

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Autores principales:	Kai Shan, Hongyan Qu, Keru Zhou, Liangfang Wang, Congmin Zhu, Haiqin Chen, Zhennan Gu, Jing Cui, Guoling Fu, Jiaqi Li, Heyan Chen, Rong Wang, Yumin Qi, Wei Chen, Yong Q. Chen
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	high-energy diet prediabetes gut microbiota metagenome metabolome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/8e5d10140e4c480580c70cad64dc5d6c
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spelling	oai:doaj.org-article:8e5d10140e4c480580c70cad64dc5d6c2021-12-02T19:47:35ZDistinct Gut Microbiota Induced by Different Fat-to-Sugar-Ratio High-Energy Diets Share Similar Pro-obesity Genetic and Metabolite Profiles in Prediabetic Mice10.1128/mSystems.00219-192379-5077https://doaj.org/article/8e5d10140e4c480580c70cad64dc5d6c2019-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00219-19https://doaj.org/toc/2379-5077ABSTRACT Gut microbiota play important roles in host metabolism, especially in diabetes. However, why different diets lead to similar diabetic states despite being associated with different microbiota is not clear. Mice were fed two high-energy diets (HED) with the same energy density but different fat-to-sugar ratios to determine the associations between the microbiota and early-stage metabolic syndrome. The two diets resulted in different microbiota but similar diabetic states. Interestingly, the microbial gene profiles were not significantly different, and many common metabolites were identified, including l-aspartic acid, cholestan-3-ol (5β, 3α), and campesterol, which have been associated with lipogenesis and inflammation. Our study suggests that different metabolic-syndrome-inducing diets may result in different microbiota but similar microbiomes and metabolomes. This suggests that the metagenome and metabolome are crucial for the prognosis and pathogenesis of obesity and metabolic syndrome. IMPORTANCE Various types of diet can lead to type 2 diabetes. The gut microbiota in type 2 diabetic patients are also different. So, two questions arise: whether there are any commonalities between gut microbiota induced by different pro-obese diets and whether these commonalities lead to disease. Here we found that high-energy diets with two different fat-to-sugar ratios can both cause obesity and prediabetes but enrich different gut microbiota. Still, these different gut microbiota have similar genetic and metabolite compositions. The microbial metabolites in common between the diets modulate lipid accumulation and macrophage inflammation in vivo and in vitro. This work suggests that studies that only use 16S rRNA amplicon sequencing to determine how the microbes respond to diet and associate with diabetic state are missing vital information.Kai ShanHongyan QuKeru ZhouLiangfang WangCongmin ZhuHaiqin ChenZhennan GuJing CuiGuoling FuJiaqi LiHeyan ChenRong WangYumin QiWei ChenYong Q. ChenAmerican Society for Microbiologyarticlehigh-energy dietprediabetesgut microbiotametagenomemetabolomeMicrobiologyQR1-502ENmSystems, Vol 4, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	high-energy diet prediabetes gut microbiota metagenome metabolome Microbiology QR1-502
spellingShingle	high-energy diet prediabetes gut microbiota metagenome metabolome Microbiology QR1-502 Kai Shan Hongyan Qu Keru Zhou Liangfang Wang Congmin Zhu Haiqin Chen Zhennan Gu Jing Cui Guoling Fu Jiaqi Li Heyan Chen Rong Wang Yumin Qi Wei Chen Yong Q. Chen Distinct Gut Microbiota Induced by Different Fat-to-Sugar-Ratio High-Energy Diets Share Similar Pro-obesity Genetic and Metabolite Profiles in Prediabetic Mice
description	ABSTRACT Gut microbiota play important roles in host metabolism, especially in diabetes. However, why different diets lead to similar diabetic states despite being associated with different microbiota is not clear. Mice were fed two high-energy diets (HED) with the same energy density but different fat-to-sugar ratios to determine the associations between the microbiota and early-stage metabolic syndrome. The two diets resulted in different microbiota but similar diabetic states. Interestingly, the microbial gene profiles were not significantly different, and many common metabolites were identified, including l-aspartic acid, cholestan-3-ol (5β, 3α), and campesterol, which have been associated with lipogenesis and inflammation. Our study suggests that different metabolic-syndrome-inducing diets may result in different microbiota but similar microbiomes and metabolomes. This suggests that the metagenome and metabolome are crucial for the prognosis and pathogenesis of obesity and metabolic syndrome. IMPORTANCE Various types of diet can lead to type 2 diabetes. The gut microbiota in type 2 diabetic patients are also different. So, two questions arise: whether there are any commonalities between gut microbiota induced by different pro-obese diets and whether these commonalities lead to disease. Here we found that high-energy diets with two different fat-to-sugar ratios can both cause obesity and prediabetes but enrich different gut microbiota. Still, these different gut microbiota have similar genetic and metabolite compositions. The microbial metabolites in common between the diets modulate lipid accumulation and macrophage inflammation in vivo and in vitro. This work suggests that studies that only use 16S rRNA amplicon sequencing to determine how the microbes respond to diet and associate with diabetic state are missing vital information.
format	article
author	Kai Shan Hongyan Qu Keru Zhou Liangfang Wang Congmin Zhu Haiqin Chen Zhennan Gu Jing Cui Guoling Fu Jiaqi Li Heyan Chen Rong Wang Yumin Qi Wei Chen Yong Q. Chen
author_facet	Kai Shan Hongyan Qu Keru Zhou Liangfang Wang Congmin Zhu Haiqin Chen Zhennan Gu Jing Cui Guoling Fu Jiaqi Li Heyan Chen Rong Wang Yumin Qi Wei Chen Yong Q. Chen
author_sort	Kai Shan
title	Distinct Gut Microbiota Induced by Different Fat-to-Sugar-Ratio High-Energy Diets Share Similar Pro-obesity Genetic and Metabolite Profiles in Prediabetic Mice
title_short	Distinct Gut Microbiota Induced by Different Fat-to-Sugar-Ratio High-Energy Diets Share Similar Pro-obesity Genetic and Metabolite Profiles in Prediabetic Mice
title_full	Distinct Gut Microbiota Induced by Different Fat-to-Sugar-Ratio High-Energy Diets Share Similar Pro-obesity Genetic and Metabolite Profiles in Prediabetic Mice
title_fullStr	Distinct Gut Microbiota Induced by Different Fat-to-Sugar-Ratio High-Energy Diets Share Similar Pro-obesity Genetic and Metabolite Profiles in Prediabetic Mice
title_full_unstemmed	Distinct Gut Microbiota Induced by Different Fat-to-Sugar-Ratio High-Energy Diets Share Similar Pro-obesity Genetic and Metabolite Profiles in Prediabetic Mice
title_sort	distinct gut microbiota induced by different fat-to-sugar-ratio high-energy diets share similar pro-obesity genetic and metabolite profiles in prediabetic mice
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/8e5d10140e4c480580c70cad64dc5d6c
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Distinct Gut Microbiota Induced by Different Fat-to-Sugar-Ratio High-Energy Diets Share Similar Pro-obesity Genetic and Metabolite Profiles in Prediabetic Mice

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