Effect of Probiotic Supplementation on Sprague Dawley Rat Liver Histopathology Fed by High Fat High Fructose Diet

Background: Non-alcoholic fatty liver disease (NAFLD) is an important cause of liver disease burden worldwide. The gastrointestinal microbiota has a close relationship with the liver as the liver is most exposed to intestinal bacteria. Microbial manipulation is a potential and effective therapy as a...

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Autores principales:	Ninung Rose Diana Kusumawati, Damianus Galih Panunggal, Maria Mexitalia, Magdalena Sidhartani, Juwita Pratiwi, Agustini Utari
Formato:	article
Lenguaje:	EN
Publicado:	Diponegoro University 2021
Materias:	probiotic nafld fibrosis histopathology Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8e5e779e05c54b559d38b6f0ceb9f249
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spelling	oai:doaj.org-article:8e5e779e05c54b559d38b6f0ceb9f2492021-11-05T16:47:51ZEffect of Probiotic Supplementation on Sprague Dawley Rat Liver Histopathology Fed by High Fat High Fructose Diet2503-217810.14710/jbtr.v7i2.11918https://doaj.org/article/8e5e779e05c54b559d38b6f0ceb9f2492021-08-01T00:00:00Zhttps://ejournal2.undip.ac.id/index.php/jbtr/article/view/11918https://doaj.org/toc/2503-2178Background: Non-alcoholic fatty liver disease (NAFLD) is an important cause of liver disease burden worldwide. The gastrointestinal microbiota has a close relationship with the liver as the liver is most exposed to intestinal bacteria. Microbial manipulation is a potential and effective therapy as an alternative in the management of NAFLD/NASH. It has been found that probiotics prevent NAFLD/NASH. However, the study about the protective effect of probiotics on NAFLD/NASH is still limited. Objective: The objective of this study is to evaluate the effect of probiotics on liver histopathology Sprague Dawley rats which given high-fat high fructose (HFHFr) diet Methods: This study is a murine-model post-test-only control study group design. The samples were 21 Sprague Dawley male rats in 7 – 8 weeks of age and were divided into three groups. The Control Group (C) was provided with a standard chow diet for eight weeks. The Non-Probiotic (NP) group was given a High-Fat High Fructose (HFHFr) diet for eight weeks. The Probiotic group (P) was given a HFHFr diet for eight weeks, and a combination of HFHFr and probiotic supplementation consisted of Lactobacillus acidophilus, Bifidobacterium longum, and Streptococcus thermophilus for the next eight weeks. Histopathological samples were obtained from liver biopsy to assess steatosis, NAFLD activity score (NAS), and fibrosis stages. Wilcoxon test was done to analyze body weight before and after treatment. We analyzed the difference in histopathological results using the Mann-Whitney test. Results: We found a significant difference in NAFL and NAS Score between NP and P group. The P group was shown to have lower trends for NAFLD and NASH than the NP group, but not for fibrosis. There is no significant difference between pre and post-test body weight. Conclusion: Probiotics supplementation has a protective effect on liver histopathology against disturbances caused by the HFHFr diet.Ninung Rose Diana KusumawatiDamianus Galih PanunggalMaria MexitaliaMagdalena SidhartaniJuwita PratiwiAgustini UtariDiponegoro UniversityarticleprobioticnafldfibrosishistopathologyMedicine (General)R5-920ENJournal of Biomedicine and Translational Research, Vol 7, Iss 2, Pp 69-73 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	probiotic nafld fibrosis histopathology Medicine (General) R5-920
spellingShingle	probiotic nafld fibrosis histopathology Medicine (General) R5-920 Ninung Rose Diana Kusumawati Damianus Galih Panunggal Maria Mexitalia Magdalena Sidhartani Juwita Pratiwi Agustini Utari Effect of Probiotic Supplementation on Sprague Dawley Rat Liver Histopathology Fed by High Fat High Fructose Diet
description	Background: Non-alcoholic fatty liver disease (NAFLD) is an important cause of liver disease burden worldwide. The gastrointestinal microbiota has a close relationship with the liver as the liver is most exposed to intestinal bacteria. Microbial manipulation is a potential and effective therapy as an alternative in the management of NAFLD/NASH. It has been found that probiotics prevent NAFLD/NASH. However, the study about the protective effect of probiotics on NAFLD/NASH is still limited. Objective: The objective of this study is to evaluate the effect of probiotics on liver histopathology Sprague Dawley rats which given high-fat high fructose (HFHFr) diet Methods: This study is a murine-model post-test-only control study group design. The samples were 21 Sprague Dawley male rats in 7 – 8 weeks of age and were divided into three groups. The Control Group (C) was provided with a standard chow diet for eight weeks. The Non-Probiotic (NP) group was given a High-Fat High Fructose (HFHFr) diet for eight weeks. The Probiotic group (P) was given a HFHFr diet for eight weeks, and a combination of HFHFr and probiotic supplementation consisted of Lactobacillus acidophilus, Bifidobacterium longum, and Streptococcus thermophilus for the next eight weeks. Histopathological samples were obtained from liver biopsy to assess steatosis, NAFLD activity score (NAS), and fibrosis stages. Wilcoxon test was done to analyze body weight before and after treatment. We analyzed the difference in histopathological results using the Mann-Whitney test. Results: We found a significant difference in NAFL and NAS Score between NP and P group. The P group was shown to have lower trends for NAFLD and NASH than the NP group, but not for fibrosis. There is no significant difference between pre and post-test body weight. Conclusion: Probiotics supplementation has a protective effect on liver histopathology against disturbances caused by the HFHFr diet.
format	article
author	Ninung Rose Diana Kusumawati Damianus Galih Panunggal Maria Mexitalia Magdalena Sidhartani Juwita Pratiwi Agustini Utari
author_facet	Ninung Rose Diana Kusumawati Damianus Galih Panunggal Maria Mexitalia Magdalena Sidhartani Juwita Pratiwi Agustini Utari
author_sort	Ninung Rose Diana Kusumawati
title	Effect of Probiotic Supplementation on Sprague Dawley Rat Liver Histopathology Fed by High Fat High Fructose Diet
title_short	Effect of Probiotic Supplementation on Sprague Dawley Rat Liver Histopathology Fed by High Fat High Fructose Diet
title_full	Effect of Probiotic Supplementation on Sprague Dawley Rat Liver Histopathology Fed by High Fat High Fructose Diet
title_fullStr	Effect of Probiotic Supplementation on Sprague Dawley Rat Liver Histopathology Fed by High Fat High Fructose Diet
title_full_unstemmed	Effect of Probiotic Supplementation on Sprague Dawley Rat Liver Histopathology Fed by High Fat High Fructose Diet
title_sort	effect of probiotic supplementation on sprague dawley rat liver histopathology fed by high fat high fructose diet
publisher	Diponegoro University
publishDate	2021
url	https://doaj.org/article/8e5e779e05c54b559d38b6f0ceb9f249
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Effect of Probiotic Supplementation on Sprague Dawley Rat Liver Histopathology Fed by High Fat High Fructose Diet

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