Comparison of oxidative stress/DNA damage in semen and blood of fertile and infertile men.

Abnormal spermatozoa frequently display typical features of oxidative stress, i.e. excessive level of reactive oxygen species (ROS) and depleted antioxidant capacity. Moreover, it has been found that a high level of oxidatively damaged DNA is associated with abnormal spermatozoa and male infertility...

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Autores principales:	Jolanta Guz, Daniel Gackowski, Marek Foksinski, Rafal Rozalski, Ewelina Zarakowska, Agnieszka Siomek, Anna Szpila, Marcin Kotzbach, Roman Kotzbach, Ryszard Olinski
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8e62fa446814452f95683fe329e7fadc
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spelling	oai:doaj.org-article:8e62fa446814452f95683fe329e7fadc2021-11-18T07:37:43ZComparison of oxidative stress/DNA damage in semen and blood of fertile and infertile men.1932-620310.1371/journal.pone.0068490https://doaj.org/article/8e62fa446814452f95683fe329e7fadc2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23874641/?tool=EBIhttps://doaj.org/toc/1932-6203Abnormal spermatozoa frequently display typical features of oxidative stress, i.e. excessive level of reactive oxygen species (ROS) and depleted antioxidant capacity. Moreover, it has been found that a high level of oxidatively damaged DNA is associated with abnormal spermatozoa and male infertility. Therefore, the aim of our study was the comparison of oxidative stress/DNA damage in semen and blood of fertile and infertile men. The broad range of parameters which describe oxidative stress and oxidatively damaged DNA and repair were analyzed in the blood plasma and seminal plasma of groups of fertile and infertile subjects. These parameters include: (i) 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanine (8-oxoGua) levels in urine; (ii) 8-oxodG level in DNA isolated from leukocytes and spermatozoa; (iii) antioxidant vitamins (A, C and E) and uric acid. Urinary excretion of 8-oxodG and 8-oxoGua and the level of oxidatively damaged DNA in leukocytes as well as the level of antioxidant vitamins were analyzed using HPLC and HPLC/GC/MS methods. The results of our study demonstrate that 8-oxodG level significantly correlated with every parameter which describe sperm quality: sperm count, motility and morphology. Moreover, the data indicate a higher level of 8-oxodG in sperm DNA compared with DNA of surrogate tissue (leukocytes) in infertile men as well as in healthy control group. For the whole study population the median values of 8-oxodG/10(6) dG were respectively 7.85 and 5.87 (p=0.000000002). Since 8-oxodG level in sperm DNA is inversely correlated with urinary excretion rate of 8-oxoGua, which is the product of OGG1 activity, we hypothesize that integrity of spermatozoa DNA may be highly dependent on OGG1 activity. No relationship between the whole body oxidative stress and that of sperm plasma was found, which suggests that the redox status of semen may be rather independent on this characteristic for other tissues.Jolanta GuzDaniel GackowskiMarek FoksinskiRafal RozalskiEwelina ZarakowskaAgnieszka SiomekAnna SzpilaMarcin KotzbachRoman KotzbachRyszard OlinskiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68490 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jolanta Guz Daniel Gackowski Marek Foksinski Rafal Rozalski Ewelina Zarakowska Agnieszka Siomek Anna Szpila Marcin Kotzbach Roman Kotzbach Ryszard Olinski Comparison of oxidative stress/DNA damage in semen and blood of fertile and infertile men.
description	Abnormal spermatozoa frequently display typical features of oxidative stress, i.e. excessive level of reactive oxygen species (ROS) and depleted antioxidant capacity. Moreover, it has been found that a high level of oxidatively damaged DNA is associated with abnormal spermatozoa and male infertility. Therefore, the aim of our study was the comparison of oxidative stress/DNA damage in semen and blood of fertile and infertile men. The broad range of parameters which describe oxidative stress and oxidatively damaged DNA and repair were analyzed in the blood plasma and seminal plasma of groups of fertile and infertile subjects. These parameters include: (i) 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanine (8-oxoGua) levels in urine; (ii) 8-oxodG level in DNA isolated from leukocytes and spermatozoa; (iii) antioxidant vitamins (A, C and E) and uric acid. Urinary excretion of 8-oxodG and 8-oxoGua and the level of oxidatively damaged DNA in leukocytes as well as the level of antioxidant vitamins were analyzed using HPLC and HPLC/GC/MS methods. The results of our study demonstrate that 8-oxodG level significantly correlated with every parameter which describe sperm quality: sperm count, motility and morphology. Moreover, the data indicate a higher level of 8-oxodG in sperm DNA compared with DNA of surrogate tissue (leukocytes) in infertile men as well as in healthy control group. For the whole study population the median values of 8-oxodG/10(6) dG were respectively 7.85 and 5.87 (p=0.000000002). Since 8-oxodG level in sperm DNA is inversely correlated with urinary excretion rate of 8-oxoGua, which is the product of OGG1 activity, we hypothesize that integrity of spermatozoa DNA may be highly dependent on OGG1 activity. No relationship between the whole body oxidative stress and that of sperm plasma was found, which suggests that the redox status of semen may be rather independent on this characteristic for other tissues.
format	article
author	Jolanta Guz Daniel Gackowski Marek Foksinski Rafal Rozalski Ewelina Zarakowska Agnieszka Siomek Anna Szpila Marcin Kotzbach Roman Kotzbach Ryszard Olinski
author_facet	Jolanta Guz Daniel Gackowski Marek Foksinski Rafal Rozalski Ewelina Zarakowska Agnieszka Siomek Anna Szpila Marcin Kotzbach Roman Kotzbach Ryszard Olinski
author_sort	Jolanta Guz
title	Comparison of oxidative stress/DNA damage in semen and blood of fertile and infertile men.
title_short	Comparison of oxidative stress/DNA damage in semen and blood of fertile and infertile men.
title_full	Comparison of oxidative stress/DNA damage in semen and blood of fertile and infertile men.
title_fullStr	Comparison of oxidative stress/DNA damage in semen and blood of fertile and infertile men.
title_full_unstemmed	Comparison of oxidative stress/DNA damage in semen and blood of fertile and infertile men.
title_sort	comparison of oxidative stress/dna damage in semen and blood of fertile and infertile men.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/8e62fa446814452f95683fe329e7fadc
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Comparison of oxidative stress/DNA damage in semen and blood of fertile and infertile men.

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