Florigen governs shoot regeneration

Abstract It is widely known that during the reproductive stage (flowering), plants do not root well. Most protocols of shoot regeneration in plants utilize juvenile tissue. Adding these two realities together encouraged us to study the role of florigen in shoot regeneration. Mature tobacco tissue th...

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Autores principales:	Yaarit Kutsher, Michal Fisler, Adi Faigenboim, Moshe Reuveni
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8e65efff45a549269c63d192452ca9d5
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spelling	oai:doaj.org-article:8e65efff45a549269c63d192452ca9d52021-12-02T16:10:38ZFlorigen governs shoot regeneration10.1038/s41598-021-93180-12045-2322https://doaj.org/article/8e65efff45a549269c63d192452ca9d52021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93180-1https://doaj.org/toc/2045-2322Abstract It is widely known that during the reproductive stage (flowering), plants do not root well. Most protocols of shoot regeneration in plants utilize juvenile tissue. Adding these two realities together encouraged us to study the role of florigen in shoot regeneration. Mature tobacco tissue that expresses the endogenous tobacco florigen mRNA regenerates poorly, while juvenile tissue that does not express the florigen regenerates shoots well. Inhibition of Nitric Oxide (NO) synthesis reduced shoot regeneration as well as promoted flowering and increased tobacco florigen level. In contrast, the addition of NO (by way of NO donor) to the tissue increased regeneration, delayed flowering, reduced tobacco florigen mRNA. Ectopic expression of florigen genes in tobacco or tomato decreased regeneration capacity significantly. Overexpression pear PcFT2 gene increased regeneration capacity. During regeneration, florigen mRNA was not changed. We conclude that florigen presence in mature tobacco leaves reduces roots and shoots regeneration and is the possible reason for the age-related decrease in regeneration capacity.Yaarit KutsherMichal FislerAdi FaigenboimMoshe ReuveniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yaarit Kutsher Michal Fisler Adi Faigenboim Moshe Reuveni Florigen governs shoot regeneration
description	Abstract It is widely known that during the reproductive stage (flowering), plants do not root well. Most protocols of shoot regeneration in plants utilize juvenile tissue. Adding these two realities together encouraged us to study the role of florigen in shoot regeneration. Mature tobacco tissue that expresses the endogenous tobacco florigen mRNA regenerates poorly, while juvenile tissue that does not express the florigen regenerates shoots well. Inhibition of Nitric Oxide (NO) synthesis reduced shoot regeneration as well as promoted flowering and increased tobacco florigen level. In contrast, the addition of NO (by way of NO donor) to the tissue increased regeneration, delayed flowering, reduced tobacco florigen mRNA. Ectopic expression of florigen genes in tobacco or tomato decreased regeneration capacity significantly. Overexpression pear PcFT2 gene increased regeneration capacity. During regeneration, florigen mRNA was not changed. We conclude that florigen presence in mature tobacco leaves reduces roots and shoots regeneration and is the possible reason for the age-related decrease in regeneration capacity.
format	article
author	Yaarit Kutsher Michal Fisler Adi Faigenboim Moshe Reuveni
author_facet	Yaarit Kutsher Michal Fisler Adi Faigenboim Moshe Reuveni
author_sort	Yaarit Kutsher
title	Florigen governs shoot regeneration
title_short	Florigen governs shoot regeneration
title_full	Florigen governs shoot regeneration
title_fullStr	Florigen governs shoot regeneration
title_full_unstemmed	Florigen governs shoot regeneration
title_sort	florigen governs shoot regeneration
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8e65efff45a549269c63d192452ca9d5
work_keys_str_mv	AT yaaritkutsher florigengovernsshootregeneration AT michalfisler florigengovernsshootregeneration AT adifaigenboim florigengovernsshootregeneration AT moshereuveni florigengovernsshootregeneration
_version_	1718384422422577152

Florigen governs shoot regeneration

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