Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts
The extraction of clary sage (<i>Salvia sclarea</i> L.) using supercritical carbon dioxide (SC-CO<sub>2</sub>) was systematically studied by using thin layer chromatography-direct bioautography (TLC-DB) and response surface methodology (RSM). The three parameters temperature,...
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| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/8e67885666a243d986669df466f716ef |
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| Sumario: | The extraction of clary sage (<i>Salvia sclarea</i> L.) using supercritical carbon dioxide (SC-CO<sub>2</sub>) was systematically studied by using thin layer chromatography-direct bioautography (TLC-DB) and response surface methodology (RSM). The three parameters temperature, pressure, and cosolvent ratio were optimized for the maximum antibacterial activity of clary sage extracts against <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) and methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). The highest inhibition zone was 7.51 mm for <i>P. aeruginosa</i> and 7.57 mm for MRSA. According to RSM analysis, the predicted optimum extraction parameters are 18.6 MPa pressure, 40 °C temperature, and 2% ethanol (EtOH) ratio. The combination of this analytical and statistical method allows saving time, money, and instrument runtime in the optimization of essential oil composition, which is tailored to a specific task and could be useful on any kind of herbs in a wide range of use from perfume manufacturing to the food industry. |
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