Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts

The extraction of clary sage (<i>Salvia sclarea</i> L.) using supercritical carbon dioxide (SC-CO<sub>2</sub>) was systematically studied by using thin layer chromatography-direct bioautography (TLC-DB) and response surface methodology (RSM). The three parameters temperature,...

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Autores principales:	Csongor Bakó, Viktória Lilla Balázs, Gyöngyi Takács, József Péter Pallos, Szilárd Pál, Béla Kocsis, Dóra Rippelné Pethő, Györgyi Horváth
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	essential oils clary sage supercritical fluid extraction thin layer chromatography-direct bioautography response surface method Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/8e67885666a243d986669df466f716ef
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spelling	oai:doaj.org-article:8e67885666a243d986669df466f716ef2021-11-11T18:27:41ZCombination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts10.3390/molecules262164491420-3049https://doaj.org/article/8e67885666a243d986669df466f716ef2021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6449https://doaj.org/toc/1420-3049The extraction of clary sage (<i>Salvia sclarea</i> L.) using supercritical carbon dioxide (SC-CO<sub>2</sub>) was systematically studied by using thin layer chromatography-direct bioautography (TLC-DB) and response surface methodology (RSM). The three parameters temperature, pressure, and cosolvent ratio were optimized for the maximum antibacterial activity of clary sage extracts against <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) and methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). The highest inhibition zone was 7.51 mm for <i>P. aeruginosa</i> and 7.57 mm for MRSA. According to RSM analysis, the predicted optimum extraction parameters are 18.6 MPa pressure, 40 °C temperature, and 2% ethanol (EtOH) ratio. The combination of this analytical and statistical method allows saving time, money, and instrument runtime in the optimization of essential oil composition, which is tailored to a specific task and could be useful on any kind of herbs in a wide range of use from perfume manufacturing to the food industry.Csongor BakóViktória Lilla BalázsGyöngyi TakácsJózsef Péter PallosSzilárd PálBéla KocsisDóra Rippelné PethőGyörgyi HorváthMDPI AGarticleessential oilsclary sagesupercritical fluid extractionthin layer chromatography-direct bioautographyresponse surface methodOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6449, p 6449 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	essential oils clary sage supercritical fluid extraction thin layer chromatography-direct bioautography response surface method Organic chemistry QD241-441
spellingShingle	essential oils clary sage supercritical fluid extraction thin layer chromatography-direct bioautography response surface method Organic chemistry QD241-441 Csongor Bakó Viktória Lilla Balázs Gyöngyi Takács József Péter Pallos Szilárd Pál Béla Kocsis Dóra Rippelné Pethő Györgyi Horváth Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts
description	The extraction of clary sage (<i>Salvia sclarea</i> L.) using supercritical carbon dioxide (SC-CO<sub>2</sub>) was systematically studied by using thin layer chromatography-direct bioautography (TLC-DB) and response surface methodology (RSM). The three parameters temperature, pressure, and cosolvent ratio were optimized for the maximum antibacterial activity of clary sage extracts against <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) and methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). The highest inhibition zone was 7.51 mm for <i>P. aeruginosa</i> and 7.57 mm for MRSA. According to RSM analysis, the predicted optimum extraction parameters are 18.6 MPa pressure, 40 °C temperature, and 2% ethanol (EtOH) ratio. The combination of this analytical and statistical method allows saving time, money, and instrument runtime in the optimization of essential oil composition, which is tailored to a specific task and could be useful on any kind of herbs in a wide range of use from perfume manufacturing to the food industry.
format	article
author	Csongor Bakó Viktória Lilla Balázs Gyöngyi Takács József Péter Pallos Szilárd Pál Béla Kocsis Dóra Rippelné Pethő Györgyi Horváth
author_facet	Csongor Bakó Viktória Lilla Balázs Gyöngyi Takács József Péter Pallos Szilárd Pál Béla Kocsis Dóra Rippelné Pethő Györgyi Horváth
author_sort	Csongor Bakó
title	Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts
title_short	Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts
title_full	Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts
title_fullStr	Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts
title_full_unstemmed	Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts
title_sort	combination of analytical and statistical methods in order to optimize antibacterial activity of clary sage supercritical fluid extracts
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8e67885666a243d986669df466f716ef
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Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts

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