High throughput method to determine the surface activity of antimicrobial polymeric materials

Surface colonization by microorganisms, combined with the rise in antibiotic resistance, is the main cause of production failures in various industries. Self-sterilising materials are deemed the best prevention of surface colonization. However, current screening methods for these sterilising materia...

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Autores principales:	Wilma van Rensburg, Wikus Ernst Laubscher, Marina Rautenbach
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Resazurin solid surface antimicrobial assay Science Q
Acceso en línea:	https://doaj.org/article/8e688090c1a64682b568137bcb62111d
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spelling	oai:doaj.org-article:8e688090c1a64682b568137bcb62111d2021-12-04T04:34:24ZHigh throughput method to determine the surface activity of antimicrobial polymeric materials2215-016110.1016/j.mex.2021.101593https://doaj.org/article/8e688090c1a64682b568137bcb62111d2021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2215016121003836https://doaj.org/toc/2215-0161Surface colonization by microorganisms, combined with the rise in antibiotic resistance, is the main cause of production failures in various industries. Self-sterilising materials are deemed the best prevention of surface colonization. However, current screening methods for these sterilising materials are laborious and time-consuming. The disk diffusion antimicrobial assay and the Japanese industrial standard method for antimicrobial activity on solid surfaces, JIS Z 2801, were compared to our modified solid surface antimicrobial assay in terms of detecting the activity of antibiotic-containing cellulose disks against four bacterial pathogens. Our novel assay circumvents the long incubation times by utilising the metabolic active dye, resazurin, to shorten the time in which antibacterial results are obtained to less than 4 h. This assay allows for increased screening to identify novel sterilising materials for combatting surface colonisation. • Disk diffusion assay could only detect the activity of small compounds that leached from the material over 20–24 h. • JIS Z 2801 was also able to detect the surface activity of non-polar compounds, thought to be inactive based on the disk diffusion results. • The resazurin solid surface antimicrobial assay could obtain the same results as the JIS Z 2801, within a shorter time and in a high-throughput 96-well plate setup.Wilma van RensburgWikus Ernst LaubscherMarina RautenbachElsevierarticleResazurin solid surface antimicrobial assayScienceQENMethodsX, Vol 8, Iss , Pp 101593- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Resazurin solid surface antimicrobial assay Science Q
spellingShingle	Resazurin solid surface antimicrobial assay Science Q Wilma van Rensburg Wikus Ernst Laubscher Marina Rautenbach High throughput method to determine the surface activity of antimicrobial polymeric materials
description	Surface colonization by microorganisms, combined with the rise in antibiotic resistance, is the main cause of production failures in various industries. Self-sterilising materials are deemed the best prevention of surface colonization. However, current screening methods for these sterilising materials are laborious and time-consuming. The disk diffusion antimicrobial assay and the Japanese industrial standard method for antimicrobial activity on solid surfaces, JIS Z 2801, were compared to our modified solid surface antimicrobial assay in terms of detecting the activity of antibiotic-containing cellulose disks against four bacterial pathogens. Our novel assay circumvents the long incubation times by utilising the metabolic active dye, resazurin, to shorten the time in which antibacterial results are obtained to less than 4 h. This assay allows for increased screening to identify novel sterilising materials for combatting surface colonisation. • Disk diffusion assay could only detect the activity of small compounds that leached from the material over 20–24 h. • JIS Z 2801 was also able to detect the surface activity of non-polar compounds, thought to be inactive based on the disk diffusion results. • The resazurin solid surface antimicrobial assay could obtain the same results as the JIS Z 2801, within a shorter time and in a high-throughput 96-well plate setup.
format	article
author	Wilma van Rensburg Wikus Ernst Laubscher Marina Rautenbach
author_facet	Wilma van Rensburg Wikus Ernst Laubscher Marina Rautenbach
author_sort	Wilma van Rensburg
title	High throughput method to determine the surface activity of antimicrobial polymeric materials
title_short	High throughput method to determine the surface activity of antimicrobial polymeric materials
title_full	High throughput method to determine the surface activity of antimicrobial polymeric materials
title_fullStr	High throughput method to determine the surface activity of antimicrobial polymeric materials
title_full_unstemmed	High throughput method to determine the surface activity of antimicrobial polymeric materials
title_sort	high throughput method to determine the surface activity of antimicrobial polymeric materials
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/8e688090c1a64682b568137bcb62111d
work_keys_str_mv	AT wilmavanrensburg highthroughputmethodtodeterminethesurfaceactivityofantimicrobialpolymericmaterials AT wikusernstlaubscher highthroughputmethodtodeterminethesurfaceactivityofantimicrobialpolymericmaterials AT marinarautenbach highthroughputmethodtodeterminethesurfaceactivityofantimicrobialpolymericmaterials
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High throughput method to determine the surface activity of antimicrobial polymeric materials

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