A shift towards pro-inflammatory CD16+ monocyte subsets with preserved cytokine production potential after kidney transplantation.

<h4>Background</h4>The presence of monocyte-macrophage lineage cells in rejecting kidney transplants is associated with worse graft outcome. At present, it is still unclear how the monocyte-macrophage related responses develop after transplantation. Here, we studied the dynamics, phenoty...

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Autores principales:	Elly J F Vereyken, Marina D Kraaij, Carla C Baan, Farhad Rezaee, Willem Weimar, Kathryn J Wood, Pieter J M Leenen, Ajda T Rowshani
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8e6ae6b286c24bbeaa4545af3147d03d
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spelling	oai:doaj.org-article:8e6ae6b286c24bbeaa4545af3147d03d2021-11-18T09:02:14ZA shift towards pro-inflammatory CD16+ monocyte subsets with preserved cytokine production potential after kidney transplantation.1932-620310.1371/journal.pone.0070152https://doaj.org/article/8e6ae6b286c24bbeaa4545af3147d03d2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23922945/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The presence of monocyte-macrophage lineage cells in rejecting kidney transplants is associated with worse graft outcome. At present, it is still unclear how the monocyte-macrophage related responses develop after transplantation. Here, we studied the dynamics, phenotypic and functional characteristics of circulating monocytes during the first 6 months after transplantation and aimed to establish the differences between kidney transplant recipients and healthy individuals.<h4>Methods</h4>Phenotype, activation status and cytokine production capacity of classical (CD14++CD16-), intermediate (CD14++CD16+) and non-classical (CD14+CD16++), monocytes were determined by flow cytometry in a cohort of 33 healthy individuals, 30 renal transplant recipients at transplantation, 19 recipients at 3 months and 16 recipients at 6 months after transplantation using a cross-sectional approach.<h4>Results</h4>The percentage of both CD16+ monocyte subsets was significantly increased in transplant recipients compared to healthy individuals, indicative of triggered innate immunity (p≤0.039). Enhanced production capacity of tumor necrosis factor-α, interferon-γ and interleukin-1β was observed by monocytes at transplantation compared to healthy individuals. Remarkably, three months post-transplant, in presence of potent immunosuppressive drugs and despite improved kidney function, interferon-γ, tumor necrosis factor-α and interleukin-10 production capacity still remained significantly increased.<h4>Conclusion</h4>Our data demonstrate a skewed balance towards pro-inflammatory CD16+ monocytes that is present at the time of transplantation and retained for at least 6 months after transplantation. This shift could be one of the important drivers of early post-transplant cellular immunity.Elly J F VereykenMarina D KraaijCarla C BaanFarhad RezaeeWillem WeimarKathryn J WoodPieter J M LeenenAjda T RowshaniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e70152 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elly J F Vereyken Marina D Kraaij Carla C Baan Farhad Rezaee Willem Weimar Kathryn J Wood Pieter J M Leenen Ajda T Rowshani A shift towards pro-inflammatory CD16+ monocyte subsets with preserved cytokine production potential after kidney transplantation.
description	<h4>Background</h4>The presence of monocyte-macrophage lineage cells in rejecting kidney transplants is associated with worse graft outcome. At present, it is still unclear how the monocyte-macrophage related responses develop after transplantation. Here, we studied the dynamics, phenotypic and functional characteristics of circulating monocytes during the first 6 months after transplantation and aimed to establish the differences between kidney transplant recipients and healthy individuals.<h4>Methods</h4>Phenotype, activation status and cytokine production capacity of classical (CD14++CD16-), intermediate (CD14++CD16+) and non-classical (CD14+CD16++), monocytes were determined by flow cytometry in a cohort of 33 healthy individuals, 30 renal transplant recipients at transplantation, 19 recipients at 3 months and 16 recipients at 6 months after transplantation using a cross-sectional approach.<h4>Results</h4>The percentage of both CD16+ monocyte subsets was significantly increased in transplant recipients compared to healthy individuals, indicative of triggered innate immunity (p≤0.039). Enhanced production capacity of tumor necrosis factor-α, interferon-γ and interleukin-1β was observed by monocytes at transplantation compared to healthy individuals. Remarkably, three months post-transplant, in presence of potent immunosuppressive drugs and despite improved kidney function, interferon-γ, tumor necrosis factor-α and interleukin-10 production capacity still remained significantly increased.<h4>Conclusion</h4>Our data demonstrate a skewed balance towards pro-inflammatory CD16+ monocytes that is present at the time of transplantation and retained for at least 6 months after transplantation. This shift could be one of the important drivers of early post-transplant cellular immunity.
format	article
author	Elly J F Vereyken Marina D Kraaij Carla C Baan Farhad Rezaee Willem Weimar Kathryn J Wood Pieter J M Leenen Ajda T Rowshani
author_facet	Elly J F Vereyken Marina D Kraaij Carla C Baan Farhad Rezaee Willem Weimar Kathryn J Wood Pieter J M Leenen Ajda T Rowshani
author_sort	Elly J F Vereyken
title	A shift towards pro-inflammatory CD16+ monocyte subsets with preserved cytokine production potential after kidney transplantation.
title_short	A shift towards pro-inflammatory CD16+ monocyte subsets with preserved cytokine production potential after kidney transplantation.
title_full	A shift towards pro-inflammatory CD16+ monocyte subsets with preserved cytokine production potential after kidney transplantation.
title_fullStr	A shift towards pro-inflammatory CD16+ monocyte subsets with preserved cytokine production potential after kidney transplantation.
title_full_unstemmed	A shift towards pro-inflammatory CD16+ monocyte subsets with preserved cytokine production potential after kidney transplantation.
title_sort	shift towards pro-inflammatory cd16+ monocyte subsets with preserved cytokine production potential after kidney transplantation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/8e6ae6b286c24bbeaa4545af3147d03d
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A shift towards pro-inflammatory CD16+ monocyte subsets with preserved cytokine production potential after kidney transplantation.

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