Methotrexate diethyl ester-loaded lipid-core nanocapsules in aqueous solution increased antineoplastic effects in resistant breast cancer cell line

Virginia C Yurgel,1,* Catiuscia P Oliveira,2,* Karine R Begnini,1 Eduarda Schultze,1 Helena S Thurow,1 Priscila MM Leon,1 Odir A Dellagostin,1 Vinicius F Campos,1 Ruy CR Beck,2 Silvia S Guterres,2 Tiago Collares,1 Adriana R Pohlmann,2–4 Fabiana K Seixas11Programa de Pós-Gradua&...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yurgel VC, Oliveira CP, Begnini KR, Schultze E, Thurow HS, Leon PMM, Dellagostin OA, Campos VF, Beck RC, Guterres SS, Collares T, Pohlmann AR, Seixas FK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8e6ee01d18de43bda3c5c687c86fa807
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8e6ee01d18de43bda3c5c687c86fa807
record_format	dspace
spelling	oai:doaj.org-article:8e6ee01d18de43bda3c5c687c86fa8072021-12-02T01:19:52ZMethotrexate diethyl ester-loaded lipid-core nanocapsules in aqueous solution increased antineoplastic effects in resistant breast cancer cell line1178-2013https://doaj.org/article/8e6ee01d18de43bda3c5c687c86fa8072014-03-01T00:00:00Zhttp://www.dovepress.com/methotrexate-diethyl-ester-loaded-lipid-core-nanocapsules-in-aqueous-s-a16249https://doaj.org/toc/1178-2013 Virginia C Yurgel,1,* Catiuscia P Oliveira,2,* Karine R Begnini,1 Eduarda Schultze,1 Helena S Thurow,1 Priscila MM Leon,1 Odir A Dellagostin,1 Vinicius F Campos,1 Ruy CR Beck,2 Silvia S Guterres,2 Tiago Collares,1 Adriana R Pohlmann,2–4 Fabiana K Seixas11Programa de Pós-Graduação em Biotecnologia (PPGB), Grupo de Pesquisa em Oncologia Celular e Molecular, Laboratório de Genômica Funcional, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil; 2Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil; 3Departamento de Química Orgânica, Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil; 4Centro de Nanociência e Nanotecnologia, CNANO-UFRGS, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil*These authors contributed equally to this workAbstract: Breast cancer is the most frequent cancer affecting women. Methotrexate (MTX) is an antimetabolic drug that remains important in the treatment of breast cancer. Its efficacy is compromised by resistance in cancer cells that occurs through a variety of mechanisms. This study evaluated apoptotic cell death and cell cycle arrest induced by an MTX derivative (MTX diethyl ester [MTX(OEt)2]) and MTX(OEt)2-loaded lipid-core nanocapsules in two MTX-resistant breast adenocarcinoma cell lines, MCF-7 and MDA-MB-231. The formulations prepared presented adequate granulometric profile. The treatment responses were evaluated through flow cytometry. Relying on the mechanism of resistance, we observed different responses between cell lines. For MCF-7 cells, MTX(OEt)2 solution and MTX(OEt)2-loaded lipid-core nanocapsules presented significantly higher apoptotic rates than untreated cells and cells incubated with unloaded lipid-core nanocapsules. For MDA-MB-231 cells, MTX(OEt)2-loaded lipid-core nanocapsules were significantly more efficient in inducing apoptosis than the solution of the free drug. S-phase cell cycle arrest was induced only by MTX(OEt)2 solution. The drug nanoencapsulation improved apoptosis induction for the cell line that presents MTX resistance by lack of transport receptors.Keywords: MTX derivative, resistance, apoptotic cell death, cell cycle arrest, nanocarriers, drug delivery, drug targetingYurgel VCOliveira CPBegnini KRSchultze EThurow HSLeon PMMDellagostin OACampos VFBeck RCGuterres SSCollares TPohlmann ARSeixas FKDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 1583-1591 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yurgel VC Oliveira CP Begnini KR Schultze E Thurow HS Leon PMM Dellagostin OA Campos VF Beck RC Guterres SS Collares T Pohlmann AR Seixas FK Methotrexate diethyl ester-loaded lipid-core nanocapsules in aqueous solution increased antineoplastic effects in resistant breast cancer cell line
description	Virginia C Yurgel,1,* Catiuscia P Oliveira,2,* Karine R Begnini,1 Eduarda Schultze,1 Helena S Thurow,1 Priscila MM Leon,1 Odir A Dellagostin,1 Vinicius F Campos,1 Ruy CR Beck,2 Silvia S Guterres,2 Tiago Collares,1 Adriana R Pohlmann,2–4 Fabiana K Seixas11Programa de Pós-Graduação em Biotecnologia (PPGB), Grupo de Pesquisa em Oncologia Celular e Molecular, Laboratório de Genômica Funcional, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil; 2Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil; 3Departamento de Química Orgânica, Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil; 4Centro de Nanociência e Nanotecnologia, CNANO-UFRGS, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil*These authors contributed equally to this workAbstract: Breast cancer is the most frequent cancer affecting women. Methotrexate (MTX) is an antimetabolic drug that remains important in the treatment of breast cancer. Its efficacy is compromised by resistance in cancer cells that occurs through a variety of mechanisms. This study evaluated apoptotic cell death and cell cycle arrest induced by an MTX derivative (MTX diethyl ester [MTX(OEt)2]) and MTX(OEt)2-loaded lipid-core nanocapsules in two MTX-resistant breast adenocarcinoma cell lines, MCF-7 and MDA-MB-231. The formulations prepared presented adequate granulometric profile. The treatment responses were evaluated through flow cytometry. Relying on the mechanism of resistance, we observed different responses between cell lines. For MCF-7 cells, MTX(OEt)2 solution and MTX(OEt)2-loaded lipid-core nanocapsules presented significantly higher apoptotic rates than untreated cells and cells incubated with unloaded lipid-core nanocapsules. For MDA-MB-231 cells, MTX(OEt)2-loaded lipid-core nanocapsules were significantly more efficient in inducing apoptosis than the solution of the free drug. S-phase cell cycle arrest was induced only by MTX(OEt)2 solution. The drug nanoencapsulation improved apoptosis induction for the cell line that presents MTX resistance by lack of transport receptors.Keywords: MTX derivative, resistance, apoptotic cell death, cell cycle arrest, nanocarriers, drug delivery, drug targeting
format	article
author	Yurgel VC Oliveira CP Begnini KR Schultze E Thurow HS Leon PMM Dellagostin OA Campos VF Beck RC Guterres SS Collares T Pohlmann AR Seixas FK
author_facet	Yurgel VC Oliveira CP Begnini KR Schultze E Thurow HS Leon PMM Dellagostin OA Campos VF Beck RC Guterres SS Collares T Pohlmann AR Seixas FK
author_sort	Yurgel VC
title	Methotrexate diethyl ester-loaded lipid-core nanocapsules in aqueous solution increased antineoplastic effects in resistant breast cancer cell line
title_short	Methotrexate diethyl ester-loaded lipid-core nanocapsules in aqueous solution increased antineoplastic effects in resistant breast cancer cell line
title_full	Methotrexate diethyl ester-loaded lipid-core nanocapsules in aqueous solution increased antineoplastic effects in resistant breast cancer cell line
title_fullStr	Methotrexate diethyl ester-loaded lipid-core nanocapsules in aqueous solution increased antineoplastic effects in resistant breast cancer cell line
title_full_unstemmed	Methotrexate diethyl ester-loaded lipid-core nanocapsules in aqueous solution increased antineoplastic effects in resistant breast cancer cell line
title_sort	methotrexate diethyl ester-loaded lipid-core nanocapsules in aqueous solution increased antineoplastic effects in resistant breast cancer cell line
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/8e6ee01d18de43bda3c5c687c86fa807
work_keys_str_mv	AT yurgelvc methotrexatediethylesterloadedlipidcorenanocapsulesinaqueoussolutionincreasedantineoplasticeffectsinresistantbreastcancercellline AT oliveiracp methotrexatediethylesterloadedlipidcorenanocapsulesinaqueoussolutionincreasedantineoplasticeffectsinresistantbreastcancercellline AT begninikr methotrexatediethylesterloadedlipidcorenanocapsulesinaqueoussolutionincreasedantineoplasticeffectsinresistantbreastcancercellline AT schultzee methotrexatediethylesterloadedlipidcorenanocapsulesinaqueoussolutionincreasedantineoplasticeffectsinresistantbreastcancercellline AT thurowhs methotrexatediethylesterloadedlipidcorenanocapsulesinaqueoussolutionincreasedantineoplasticeffectsinresistantbreastcancercellline AT leonpmm methotrexatediethylesterloadedlipidcorenanocapsulesinaqueoussolutionincreasedantineoplasticeffectsinresistantbreastcancercellline AT dellagostinoa methotrexatediethylesterloadedlipidcorenanocapsulesinaqueoussolutionincreasedantineoplasticeffectsinresistantbreastcancercellline AT camposvf methotrexatediethylesterloadedlipidcorenanocapsulesinaqueoussolutionincreasedantineoplasticeffectsinresistantbreastcancercellline AT beckrc methotrexatediethylesterloadedlipidcorenanocapsulesinaqueoussolutionincreasedantineoplasticeffectsinresistantbreastcancercellline AT guterresss methotrexatediethylesterloadedlipidcorenanocapsulesinaqueoussolutionincreasedantineoplasticeffectsinresistantbreastcancercellline AT collarest methotrexatediethylesterloadedlipidcorenanocapsulesinaqueoussolutionincreasedantineoplasticeffectsinresistantbreastcancercellline AT pohlmannar methotrexatediethylesterloadedlipidcorenanocapsulesinaqueoussolutionincreasedantineoplasticeffectsinresistantbreastcancercellline AT seixasfk methotrexatediethylesterloadedlipidcorenanocapsulesinaqueoussolutionincreasedantineoplasticeffectsinresistantbreastcancercellline
_version_	1718403150591819776

Methotrexate diethyl ester-loaded lipid-core nanocapsules in aqueous solution increased antineoplastic effects in resistant breast cancer cell line

Ejemplares similares