Cell survival and DNA damage repair are promoted in the human blood thanatotranscriptome shortly after death

Abstract RNA analysis of post-mortem tissues, or thanatotranscriptomics, has become a topic of interest in forensic science due to the essential information it can provide in forensic investigations. Several studies have previously investigated the effect of death on gene transcription, but it has n...

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Autores principales:	Laura G. Antiga, Lode Sibbens, Yasmina Abakkouy, Ronny Decorte, Wouter Van Den Bogaert, Wim Van de Voorde, Bram Bekaert
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8e6f164102d14b689cfc6023b48e0f9a
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spelling	oai:doaj.org-article:8e6f164102d14b689cfc6023b48e0f9a2021-12-02T16:45:54ZCell survival and DNA damage repair are promoted in the human blood thanatotranscriptome shortly after death10.1038/s41598-021-96095-z2045-2322https://doaj.org/article/8e6f164102d14b689cfc6023b48e0f9a2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96095-zhttps://doaj.org/toc/2045-2322Abstract RNA analysis of post-mortem tissues, or thanatotranscriptomics, has become a topic of interest in forensic science due to the essential information it can provide in forensic investigations. Several studies have previously investigated the effect of death on gene transcription, but it has never been conducted with samples of the same individual. For the first time, a longitudinal mRNA expression analysis study was performed with post-mortem human blood samples from individuals with a known time of death. The results reveal that, after death, two clearly differentiated groups of up- and down-regulated genes can be detected. Pathway analysis suggests active processes that promote cell survival and DNA damage repair, rather than passive degradation, are the source of early post-mortem changes of gene expression in blood. In addition, a generalized linear model with an elastic net restriction predicted post-mortem interval with a root mean square error of 4.75 h. In conclusion, we demonstrate that post-mortem gene expression data can be used as biomarkers to estimate the post-mortem interval though further validation using independent sample sets is required before use in forensic casework.Laura G. AntigaLode SibbensYasmina AbakkouyRonny DecorteWouter Van Den BogaertWim Van de VoordeBram BekaertNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laura G. Antiga Lode Sibbens Yasmina Abakkouy Ronny Decorte Wouter Van Den Bogaert Wim Van de Voorde Bram Bekaert Cell survival and DNA damage repair are promoted in the human blood thanatotranscriptome shortly after death
description	Abstract RNA analysis of post-mortem tissues, or thanatotranscriptomics, has become a topic of interest in forensic science due to the essential information it can provide in forensic investigations. Several studies have previously investigated the effect of death on gene transcription, but it has never been conducted with samples of the same individual. For the first time, a longitudinal mRNA expression analysis study was performed with post-mortem human blood samples from individuals with a known time of death. The results reveal that, after death, two clearly differentiated groups of up- and down-regulated genes can be detected. Pathway analysis suggests active processes that promote cell survival and DNA damage repair, rather than passive degradation, are the source of early post-mortem changes of gene expression in blood. In addition, a generalized linear model with an elastic net restriction predicted post-mortem interval with a root mean square error of 4.75 h. In conclusion, we demonstrate that post-mortem gene expression data can be used as biomarkers to estimate the post-mortem interval though further validation using independent sample sets is required before use in forensic casework.
format	article
author	Laura G. Antiga Lode Sibbens Yasmina Abakkouy Ronny Decorte Wouter Van Den Bogaert Wim Van de Voorde Bram Bekaert
author_facet	Laura G. Antiga Lode Sibbens Yasmina Abakkouy Ronny Decorte Wouter Van Den Bogaert Wim Van de Voorde Bram Bekaert
author_sort	Laura G. Antiga
title	Cell survival and DNA damage repair are promoted in the human blood thanatotranscriptome shortly after death
title_short	Cell survival and DNA damage repair are promoted in the human blood thanatotranscriptome shortly after death
title_full	Cell survival and DNA damage repair are promoted in the human blood thanatotranscriptome shortly after death
title_fullStr	Cell survival and DNA damage repair are promoted in the human blood thanatotranscriptome shortly after death
title_full_unstemmed	Cell survival and DNA damage repair are promoted in the human blood thanatotranscriptome shortly after death
title_sort	cell survival and dna damage repair are promoted in the human blood thanatotranscriptome shortly after death
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8e6f164102d14b689cfc6023b48e0f9a
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Cell survival and DNA damage repair are promoted in the human blood thanatotranscriptome shortly after death

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