Diterpene Resin Acids and Olefins in Calabrian Pine (Pinus nigra subsp. laricio (Poiret) Maire) Oleoresin: GC-MS Profiling of Major Diterpenoids in Different Plant Organs, Molecular Identification and Expression Analysis of Diterpene Synthase Genes

A quali-quantitative analysis of diterpenoid composition in tissues obtained from different organs of Pinus nigra subsp. laricio (Poiret) Maire (Calabrian pine) was carried out. Diterpene resin acids were the most abundant diterpenoids across all the examined ti...

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Autores principales:	Enrica Alicandri, Stefano Covino, Bartolomeo Sebastiani, Anna Rita Paolacci, Maurizio Badiani, Francesco Manti, Carmelo Peter Bonsignore, Agostino Sorgonà, Mario Ciaffi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	diterpenoids diterpene synthase <i>Pinus nigra</i> subsp. <i>laricio</i> (Poiret) Maire Calabrian pine pine oleoresin genomic organization of gymnosperm diterpene synthases Botany QK1-989
Acceso en línea:	https://doaj.org/article/8e720c66842d4d4f9915112442380143
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spelling	oai:doaj.org-article:8e720c66842d4d4f99151124423801432021-11-25T18:46:13ZDiterpene Resin Acids and Olefins in Calabrian Pine (<i>Pinus nigra</i> subsp. <i>laricio</i> (Poiret) Maire) Oleoresin: GC-MS Profiling of Major Diterpenoids in Different Plant Organs, Molecular Identification and Expression Analysis of Diterpene Synthase Genes10.3390/plants101123912223-7747https://doaj.org/article/8e720c66842d4d4f99151124423801432021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2391https://doaj.org/toc/2223-7747A quali-quantitative analysis of diterpenoid composition in tissues obtained from different organs of <i>Pinus nigra</i> subsp. <i>laricio</i> (Poiret) Maire (Calabrian pine) was carried out. Diterpene resin acids were the most abundant diterpenoids across all the examined tissues. The same nine diterpene resin acids were always found, with the abietane type prevailing on the pimarane type, although their quantitative distribution was found to be remarkably tissue-specific. The scrutiny of the available literature revealed species specificity as well. A phylogeny-based approach allowed us to isolate four cDNAs coding for diterpene synthases in Calabrian pine, each of which belonging to one of the four groups into which the d3 clade of the plants’ terpene synthases family can be divided. The deduced amino acid sequences allowed predicting that both monofunctional and bifunctional diterpene synthases are involved in the biosynthesis of diterpene resin acids in Calabrian pine. Transcript profiling revealed differential expression across the different tissues and was found to be consistent with the corresponding diterpenoid profiles. The isolation of the complete genomic sequences and the determination of their exon/intron structures allowed us to place the diterpene synthase genes from Calabrian pine on the background of current ideas on the functional evolution of diterpene synthases in Gymnosperms.Enrica AlicandriStefano CovinoBartolomeo SebastianiAnna Rita PaolacciMaurizio BadianiFrancesco MantiCarmelo Peter BonsignoreAgostino SorgonàMario CiaffiMDPI AGarticlediterpenoidsditerpene synthase<i>Pinus nigra</i> subsp. <i>laricio</i> (Poiret) MaireCalabrian pinepine oleoresingenomic organization of gymnosperm diterpene synthasesBotanyQK1-989ENPlants, Vol 10, Iss 2391, p 2391 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diterpenoids diterpene synthase <i>Pinus nigra</i> subsp. <i>laricio</i> (Poiret) Maire Calabrian pine pine oleoresin genomic organization of gymnosperm diterpene synthases Botany QK1-989
spellingShingle	diterpenoids diterpene synthase <i>Pinus nigra</i> subsp. <i>laricio</i> (Poiret) Maire Calabrian pine pine oleoresin genomic organization of gymnosperm diterpene synthases Botany QK1-989 Enrica Alicandri Stefano Covino Bartolomeo Sebastiani Anna Rita Paolacci Maurizio Badiani Francesco Manti Carmelo Peter Bonsignore Agostino Sorgonà Mario Ciaffi Diterpene Resin Acids and Olefins in Calabrian Pine (<i>Pinus nigra</i> subsp. <i>laricio</i> (Poiret) Maire) Oleoresin: GC-MS Profiling of Major Diterpenoids in Different Plant Organs, Molecular Identification and Expression Analysis of Diterpene Synthase Genes
description	A quali-quantitative analysis of diterpenoid composition in tissues obtained from different organs of <i>Pinus nigra</i> subsp. <i>laricio</i> (Poiret) Maire (Calabrian pine) was carried out. Diterpene resin acids were the most abundant diterpenoids across all the examined tissues. The same nine diterpene resin acids were always found, with the abietane type prevailing on the pimarane type, although their quantitative distribution was found to be remarkably tissue-specific. The scrutiny of the available literature revealed species specificity as well. A phylogeny-based approach allowed us to isolate four cDNAs coding for diterpene synthases in Calabrian pine, each of which belonging to one of the four groups into which the d3 clade of the plants’ terpene synthases family can be divided. The deduced amino acid sequences allowed predicting that both monofunctional and bifunctional diterpene synthases are involved in the biosynthesis of diterpene resin acids in Calabrian pine. Transcript profiling revealed differential expression across the different tissues and was found to be consistent with the corresponding diterpenoid profiles. The isolation of the complete genomic sequences and the determination of their exon/intron structures allowed us to place the diterpene synthase genes from Calabrian pine on the background of current ideas on the functional evolution of diterpene synthases in Gymnosperms.
format	article
author	Enrica Alicandri Stefano Covino Bartolomeo Sebastiani Anna Rita Paolacci Maurizio Badiani Francesco Manti Carmelo Peter Bonsignore Agostino Sorgonà Mario Ciaffi
author_facet	Enrica Alicandri Stefano Covino Bartolomeo Sebastiani Anna Rita Paolacci Maurizio Badiani Francesco Manti Carmelo Peter Bonsignore Agostino Sorgonà Mario Ciaffi
author_sort	Enrica Alicandri
title	Diterpene Resin Acids and Olefins in Calabrian Pine (<i>Pinus nigra</i> subsp. <i>laricio</i> (Poiret) Maire) Oleoresin: GC-MS Profiling of Major Diterpenoids in Different Plant Organs, Molecular Identification and Expression Analysis of Diterpene Synthase Genes
title_short	Diterpene Resin Acids and Olefins in Calabrian Pine (<i>Pinus nigra</i> subsp. <i>laricio</i> (Poiret) Maire) Oleoresin: GC-MS Profiling of Major Diterpenoids in Different Plant Organs, Molecular Identification and Expression Analysis of Diterpene Synthase Genes
title_full	Diterpene Resin Acids and Olefins in Calabrian Pine (<i>Pinus nigra</i> subsp. <i>laricio</i> (Poiret) Maire) Oleoresin: GC-MS Profiling of Major Diterpenoids in Different Plant Organs, Molecular Identification and Expression Analysis of Diterpene Synthase Genes
title_fullStr	Diterpene Resin Acids and Olefins in Calabrian Pine (<i>Pinus nigra</i> subsp. <i>laricio</i> (Poiret) Maire) Oleoresin: GC-MS Profiling of Major Diterpenoids in Different Plant Organs, Molecular Identification and Expression Analysis of Diterpene Synthase Genes
title_full_unstemmed	Diterpene Resin Acids and Olefins in Calabrian Pine (<i>Pinus nigra</i> subsp. <i>laricio</i> (Poiret) Maire) Oleoresin: GC-MS Profiling of Major Diterpenoids in Different Plant Organs, Molecular Identification and Expression Analysis of Diterpene Synthase Genes
title_sort	diterpene resin acids and olefins in calabrian pine (<i>pinus nigra</i> subsp. <i>laricio</i> (poiret) maire) oleoresin: gc-ms profiling of major diterpenoids in different plant organs, molecular identification and expression analysis of diterpene synthase genes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8e720c66842d4d4f9915112442380143
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Diterpene Resin Acids and Olefins in Calabrian Pine (<i>Pinus nigra</i> subsp. <i>laricio</i> (Poiret) Maire) Oleoresin: GC-MS Profiling of Major Diterpenoids in Different Plant Organs, Molecular Identification and Expression Analysis of Diterpene Synthase Genes

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