Emergence of Enteroaggregative <named-content content-type="genus-species">Escherichia coli</named-content> within the ST131 Lineage as a Cause of Extraintestinal Infections
ABSTRACT Escherichia coli sequence type 131 (ST131) is a major cause of urinary and bloodstream infections. Its association with extended-spectrum β-lactamases (ESBLs) significantly complicates treatment. Its best-described component is the rapidly expanding H30Rx clade, containing allele 30 of the...
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American Society for Microbiology
2020
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oai:doaj.org-article:8e75418bff19499db3afd52dc51986a12021-11-15T15:56:47ZEmergence of Enteroaggregative <named-content content-type="genus-species">Escherichia coli</named-content> within the ST131 Lineage as a Cause of Extraintestinal Infections10.1128/mBio.00353-202150-7511https://doaj.org/article/8e75418bff19499db3afd52dc51986a12020-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00353-20https://doaj.org/toc/2150-7511ABSTRACT Escherichia coli sequence type 131 (ST131) is a major cause of urinary and bloodstream infections. Its association with extended-spectrum β-lactamases (ESBLs) significantly complicates treatment. Its best-described component is the rapidly expanding H30Rx clade, containing allele 30 of the type 1 fimbrial adhesin gene fimH. This lineage appears to have emerged in the United States and spread around the world in part due to the acquisition of the ESBL-encoding blaCTX-M-15 gene and resistance to fluoroquinolones. However, non-H30 ST131 sublineages with other acquired CTX-M-type resistance genes are also emerging. Based on whole-genome analyses, we describe here the presence of an (fimH) H27 E. coli ST131 sublineage that has recently caused an outbreak of community-acquired bacteremia and recurrent urinary tract infections (UTIs) in Denmark. This sublineage has acquired both a virulence plasmid (pAA) that defines the enteroaggregative E. coli (EAEC) diarrheagenic pathotype and multiple genes associated with extraintestinal E. coli (ExPEC); combined, these traits have made this particular ST131 sublineage successful at colonizing its human host and causing recurrent UTI. Moreover, using a historic World Health Organization (WHO) E. coli collection and publicly available genome sequences, we identified a global H27 EAEC ST131 sublineage that dates back as far as 1998. Most H27 EAEC ST131 isolates harbor pAA or pAA-like plasmids, and our analysis strongly implies a single ancestral acquisition among these isolates. These findings illustrate both the profound plasticity of this important pathogenic E. coli ST131 H27 sublineage and genetic acquisitions of EAEC-specific virulence traits that likely confer an enhanced ability to cause intestinal colonization. IMPORTANCE E. coli ST131 is an important extraintestinal pathogenic lineage. A signature characteristic of ST131 is its ability to asymptomatically colonize the gastrointestinal tract and then opportunistically cause extraintestinal infections, such as cystitis, pyelonephritis, and urosepsis. In this study, we identified an ST131 H27 sublineage that has acquired the enteroaggregative diarrheagenic phenotype, spread across multiple continents, and caused multiple outbreaks of community-acquired ESBL-associated bloodstream infections in Denmark. The strain’s ability to both cause diarrhea and innocuously colonize the human gastrointestinal tract may facilitate its dissemination and establishment in the community.Erik J. BollSøren Overballe-PetersenHenrik HasmanLouise RoerKim NgFlemming ScheutzAnette M. HammerumArnold DunguFrank HansenThor B. JohannesenAbigail JohnsonDivek T. NairBerit LiljeDennis S. HansenKaren A. KrogfeltTimothy J. JohnsonLance B. PriceJames R. JohnsonCarsten StruveBente OlesenMarc SteggerAmerican Society for MicrobiologyarticleE. coliESBLgenomicH27resistanceST131MicrobiologyQR1-502ENmBio, Vol 11, Iss 3 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
E. coli ESBL genomic H27 resistance ST131 Microbiology QR1-502 |
| spellingShingle |
E. coli ESBL genomic H27 resistance ST131 Microbiology QR1-502 Erik J. Boll Søren Overballe-Petersen Henrik Hasman Louise Roer Kim Ng Flemming Scheutz Anette M. Hammerum Arnold Dungu Frank Hansen Thor B. Johannesen Abigail Johnson Divek T. Nair Berit Lilje Dennis S. Hansen Karen A. Krogfelt Timothy J. Johnson Lance B. Price James R. Johnson Carsten Struve Bente Olesen Marc Stegger Emergence of Enteroaggregative <named-content content-type="genus-species">Escherichia coli</named-content> within the ST131 Lineage as a Cause of Extraintestinal Infections |
| description |
ABSTRACT Escherichia coli sequence type 131 (ST131) is a major cause of urinary and bloodstream infections. Its association with extended-spectrum β-lactamases (ESBLs) significantly complicates treatment. Its best-described component is the rapidly expanding H30Rx clade, containing allele 30 of the type 1 fimbrial adhesin gene fimH. This lineage appears to have emerged in the United States and spread around the world in part due to the acquisition of the ESBL-encoding blaCTX-M-15 gene and resistance to fluoroquinolones. However, non-H30 ST131 sublineages with other acquired CTX-M-type resistance genes are also emerging. Based on whole-genome analyses, we describe here the presence of an (fimH) H27 E. coli ST131 sublineage that has recently caused an outbreak of community-acquired bacteremia and recurrent urinary tract infections (UTIs) in Denmark. This sublineage has acquired both a virulence plasmid (pAA) that defines the enteroaggregative E. coli (EAEC) diarrheagenic pathotype and multiple genes associated with extraintestinal E. coli (ExPEC); combined, these traits have made this particular ST131 sublineage successful at colonizing its human host and causing recurrent UTI. Moreover, using a historic World Health Organization (WHO) E. coli collection and publicly available genome sequences, we identified a global H27 EAEC ST131 sublineage that dates back as far as 1998. Most H27 EAEC ST131 isolates harbor pAA or pAA-like plasmids, and our analysis strongly implies a single ancestral acquisition among these isolates. These findings illustrate both the profound plasticity of this important pathogenic E. coli ST131 H27 sublineage and genetic acquisitions of EAEC-specific virulence traits that likely confer an enhanced ability to cause intestinal colonization. IMPORTANCE E. coli ST131 is an important extraintestinal pathogenic lineage. A signature characteristic of ST131 is its ability to asymptomatically colonize the gastrointestinal tract and then opportunistically cause extraintestinal infections, such as cystitis, pyelonephritis, and urosepsis. In this study, we identified an ST131 H27 sublineage that has acquired the enteroaggregative diarrheagenic phenotype, spread across multiple continents, and caused multiple outbreaks of community-acquired ESBL-associated bloodstream infections in Denmark. The strain’s ability to both cause diarrhea and innocuously colonize the human gastrointestinal tract may facilitate its dissemination and establishment in the community. |
| format |
article |
| author |
Erik J. Boll Søren Overballe-Petersen Henrik Hasman Louise Roer Kim Ng Flemming Scheutz Anette M. Hammerum Arnold Dungu Frank Hansen Thor B. Johannesen Abigail Johnson Divek T. Nair Berit Lilje Dennis S. Hansen Karen A. Krogfelt Timothy J. Johnson Lance B. Price James R. Johnson Carsten Struve Bente Olesen Marc Stegger |
| author_facet |
Erik J. Boll Søren Overballe-Petersen Henrik Hasman Louise Roer Kim Ng Flemming Scheutz Anette M. Hammerum Arnold Dungu Frank Hansen Thor B. Johannesen Abigail Johnson Divek T. Nair Berit Lilje Dennis S. Hansen Karen A. Krogfelt Timothy J. Johnson Lance B. Price James R. Johnson Carsten Struve Bente Olesen Marc Stegger |
| author_sort |
Erik J. Boll |
| title |
Emergence of Enteroaggregative <named-content content-type="genus-species">Escherichia coli</named-content> within the ST131 Lineage as a Cause of Extraintestinal Infections |
| title_short |
Emergence of Enteroaggregative <named-content content-type="genus-species">Escherichia coli</named-content> within the ST131 Lineage as a Cause of Extraintestinal Infections |
| title_full |
Emergence of Enteroaggregative <named-content content-type="genus-species">Escherichia coli</named-content> within the ST131 Lineage as a Cause of Extraintestinal Infections |
| title_fullStr |
Emergence of Enteroaggregative <named-content content-type="genus-species">Escherichia coli</named-content> within the ST131 Lineage as a Cause of Extraintestinal Infections |
| title_full_unstemmed |
Emergence of Enteroaggregative <named-content content-type="genus-species">Escherichia coli</named-content> within the ST131 Lineage as a Cause of Extraintestinal Infections |
| title_sort |
emergence of enteroaggregative <named-content content-type="genus-species">escherichia coli</named-content> within the st131 lineage as a cause of extraintestinal infections |
| publisher |
American Society for Microbiology |
| publishDate |
2020 |
| url |
https://doaj.org/article/8e75418bff19499db3afd52dc51986a1 |
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