Qingluoyin granules protect against adjuvant-induced arthritis in rats via downregulating the CXCL12/CXCR4-NF-κB signalling pathway

Context Qingluoyin (QLY) is a traditional Chinese medicine (TCM) formula which has been used in treating human rheumatoid arthritis (RA) for years in China. Objective This study investigates the effect of QLY granules on adjuvant arthritis (AA) and the possible mechanism. Materials and methods Sprag...

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Autores principales:	Min Si, Zheng Ma, Jie Zhang, Xinwei Li, Rui Li, Chao Wang, Huiyu Jia, Shengyong Luo
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	rheumatoid arthritis adjuvant arthritis inflammatory cytokine traditional chinese medicine Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/8e7a6c8c6c124c9cb09616dd98634e6f
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spelling	oai:doaj.org-article:8e7a6c8c6c124c9cb09616dd98634e6f2021-11-04T15:00:42ZQingluoyin granules protect against adjuvant-induced arthritis in rats via downregulating the CXCL12/CXCR4-NF-κB signalling pathway1388-02091744-511610.1080/13880209.2021.1991386https://doaj.org/article/8e7a6c8c6c124c9cb09616dd98634e6f2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2021.1991386https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context Qingluoyin (QLY) is a traditional Chinese medicine (TCM) formula which has been used in treating human rheumatoid arthritis (RA) for years in China. Objective This study investigates the effect of QLY granules on adjuvant arthritis (AA) and the possible mechanism. Materials and methods Sprague-Dawley (SD) rats were injected with Complete Freund’s adjuvant (CFA) to induce the AA model. After the onset of arthritis, rats received intragastric administrations of the QLY granules (1.35, 2.70, and 5.40 g/kg) or Tripterygium glycosides (TG) tablets (positive drug, 10 mg/kg) for 14 d. After 28 d immunization, the symptoms, inflammatory parameters and molecular mechanisms were investigated. Results In the QLY granule (1.35, 2.70, and 5.40 g/kg) therapy groups, the arthritis index decreased to 6.30 ± 2.06, 5.80 ± 1.55, 5.30 ± 1.16 compared with the model (9.00 ± 3.01), paw swelling decreased to 1.56 ± 0.40, 1.28 ± 0.38, 1.12 ± 0.41 mL compared with the model (2.22 ± 0.73 mL). QLY granules (1.35, 2.70 and 5.40 g/kg) significantly reduced the thymus and the spleen indexes, inhibited the production of pro-inflammatory cytokines, and alleviated the pathological changes of joints compared with the model group. Furthermore, the treatment of QLY granules (2.70 and 5.40 g/kg) markedly inhibited CXCL12, CXCR4 (in spleen and synovium) and p-NF-κB p65 (in synovium) protein expression of AA rats. Conclusions QLY granules have obvious therapeutic effects on AA rats, which may be associated with downregulating the CXCL12/CXCR4-NF-κB signalling pathway. QLY granules can be used as a candidate for the treatment of RA, which deserves further study.Min SiZheng MaJie ZhangXinwei LiRui LiChao WangHuiyu JiaShengyong LuoTaylor & Francis Grouparticlerheumatoid arthritisadjuvant arthritisinflammatory cytokinetraditional chinese medicineTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 59, Iss 1, Pp 1441-1451 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rheumatoid arthritis adjuvant arthritis inflammatory cytokine traditional chinese medicine Therapeutics. Pharmacology RM1-950
spellingShingle	rheumatoid arthritis adjuvant arthritis inflammatory cytokine traditional chinese medicine Therapeutics. Pharmacology RM1-950 Min Si Zheng Ma Jie Zhang Xinwei Li Rui Li Chao Wang Huiyu Jia Shengyong Luo Qingluoyin granules protect against adjuvant-induced arthritis in rats via downregulating the CXCL12/CXCR4-NF-κB signalling pathway
description	Context Qingluoyin (QLY) is a traditional Chinese medicine (TCM) formula which has been used in treating human rheumatoid arthritis (RA) for years in China. Objective This study investigates the effect of QLY granules on adjuvant arthritis (AA) and the possible mechanism. Materials and methods Sprague-Dawley (SD) rats were injected with Complete Freund’s adjuvant (CFA) to induce the AA model. After the onset of arthritis, rats received intragastric administrations of the QLY granules (1.35, 2.70, and 5.40 g/kg) or Tripterygium glycosides (TG) tablets (positive drug, 10 mg/kg) for 14 d. After 28 d immunization, the symptoms, inflammatory parameters and molecular mechanisms were investigated. Results In the QLY granule (1.35, 2.70, and 5.40 g/kg) therapy groups, the arthritis index decreased to 6.30 ± 2.06, 5.80 ± 1.55, 5.30 ± 1.16 compared with the model (9.00 ± 3.01), paw swelling decreased to 1.56 ± 0.40, 1.28 ± 0.38, 1.12 ± 0.41 mL compared with the model (2.22 ± 0.73 mL). QLY granules (1.35, 2.70 and 5.40 g/kg) significantly reduced the thymus and the spleen indexes, inhibited the production of pro-inflammatory cytokines, and alleviated the pathological changes of joints compared with the model group. Furthermore, the treatment of QLY granules (2.70 and 5.40 g/kg) markedly inhibited CXCL12, CXCR4 (in spleen and synovium) and p-NF-κB p65 (in synovium) protein expression of AA rats. Conclusions QLY granules have obvious therapeutic effects on AA rats, which may be associated with downregulating the CXCL12/CXCR4-NF-κB signalling pathway. QLY granules can be used as a candidate for the treatment of RA, which deserves further study.
format	article
author	Min Si Zheng Ma Jie Zhang Xinwei Li Rui Li Chao Wang Huiyu Jia Shengyong Luo
author_facet	Min Si Zheng Ma Jie Zhang Xinwei Li Rui Li Chao Wang Huiyu Jia Shengyong Luo
author_sort	Min Si
title	Qingluoyin granules protect against adjuvant-induced arthritis in rats via downregulating the CXCL12/CXCR4-NF-κB signalling pathway
title_short	Qingluoyin granules protect against adjuvant-induced arthritis in rats via downregulating the CXCL12/CXCR4-NF-κB signalling pathway
title_full	Qingluoyin granules protect against adjuvant-induced arthritis in rats via downregulating the CXCL12/CXCR4-NF-κB signalling pathway
title_fullStr	Qingluoyin granules protect against adjuvant-induced arthritis in rats via downregulating the CXCL12/CXCR4-NF-κB signalling pathway
title_full_unstemmed	Qingluoyin granules protect against adjuvant-induced arthritis in rats via downregulating the CXCL12/CXCR4-NF-κB signalling pathway
title_sort	qingluoyin granules protect against adjuvant-induced arthritis in rats via downregulating the cxcl12/cxcr4-nf-κb signalling pathway
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/8e7a6c8c6c124c9cb09616dd98634e6f
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Qingluoyin granules protect against adjuvant-induced arthritis in rats via downregulating the CXCL12/CXCR4-NF-κB signalling pathway

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