Effect of superparamagnetic iron oxide nanoparticles on fluidity and phase transition of phosphatidylcholine liposomal membranes

Poornima Budime Santhosh,1,* Barbara Dra&scaron;ler,2,* Damjana Drobne,2 Mateja Erdani Kreft,3 Slavko Kralj,4 Darko Makovec,4 Nata&scaron;a Poklar Ulrih1,5 1Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, 2Department of Biology, Biotechnical Faculty,...

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Autores principales:	Santhosh PB, Drašler B, Drobne D, Kreft ME, Kralj S, Makovec D, Ulrih NP
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8e7f3dcd2a824ad9bee113a038266754
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spelling	oai:doaj.org-article:8e7f3dcd2a824ad9bee113a0382667542021-12-02T04:28:15ZEffect of superparamagnetic iron oxide nanoparticles on fluidity and phase transition of phosphatidylcholine liposomal membranes1178-2013https://doaj.org/article/8e7f3dcd2a824ad9bee113a0382667542015-09-01T00:00:00Zhttps://www.dovepress.com/effect-of-superparamagnetic-iron-oxide-nanoparticles-on-fluidity-and-p-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Poornima Budime Santhosh,1,* Barbara Dra&scaron;ler,2,* Damjana Drobne,2 Mateja Erdani Kreft,3 Slavko Kralj,4 Darko Makovec,4 Nata&scaron;a Poklar Ulrih1,5 1Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, 2Department of Biology, Biotechnical Faculty, University of Ljubljana, 3Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, 4Department for Materials Synthesis, Jožef Stefan Institute, 5Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Ljubljana, Slovenia *These authors share equal first authorship Abstract: Superparamagnetic iron oxide nanoparticles (SPIONs) with multifunctional properties have shown great promise in theranostics. The aim of our work was to compare the effects of SPIONs on the fluidity and phase transition of the liposomal membranes prepared with zwitterionic phosphatidylcholine lipids. In order to study if the surface modification of SPIONs has any influence on these membrane properties, we have used four types of differently functionalized SPIONs, such as: plain SPIONs (primary size was shown to be ~11 nm), silica-coated SPIONs, SPIONs coated with silica and functionalized with positively charged amino groups or negatively charged carboxyl groups (the primary size of all the surface-modified SPIONs was ~20 nm). Small unilamellar vesicles prepared with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine lipids and multilamellar vesicles prepared with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine lipids were encapsulated or incubated with the plain and surface-modified SPIONs to determine the fluidity and phase transition temperature of the bilayer lipids, respectively. Fluorescent anisotropy and differential scanning calorimetric measurements of the liposomes that were either encapsulated or incubated with the suspension of SPIONs did not show a significant difference in the lipid ordering and fluidity; though the encapsulated SPIONs showed a slightly increased effect on the fluidity of the model membranes in comparison with the incubated SPIONs. This indicates the low potential of the SPIONs to interact with the nontargeted cell membranes, which is a desirable factor for in vivo applications. Keywords: encapsulated SPIONs, incubated SPIONs, zwitterionic liposomes, membrane integrity, phase behaviorSanthosh PBDrašler BDrobne DKreft MEKralj SMakovec DUlrih NPDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 6089-6104 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Santhosh PB Drašler B Drobne D Kreft ME Kralj S Makovec D Ulrih NP Effect of superparamagnetic iron oxide nanoparticles on fluidity and phase transition of phosphatidylcholine liposomal membranes
description	Poornima Budime Santhosh,1,* Barbara Dra&scaron;ler,2,* Damjana Drobne,2 Mateja Erdani Kreft,3 Slavko Kralj,4 Darko Makovec,4 Nata&scaron;a Poklar Ulrih1,5 1Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, 2Department of Biology, Biotechnical Faculty, University of Ljubljana, 3Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, 4Department for Materials Synthesis, Jožef Stefan Institute, 5Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Ljubljana, Slovenia *These authors share equal first authorship Abstract: Superparamagnetic iron oxide nanoparticles (SPIONs) with multifunctional properties have shown great promise in theranostics. The aim of our work was to compare the effects of SPIONs on the fluidity and phase transition of the liposomal membranes prepared with zwitterionic phosphatidylcholine lipids. In order to study if the surface modification of SPIONs has any influence on these membrane properties, we have used four types of differently functionalized SPIONs, such as: plain SPIONs (primary size was shown to be ~11 nm), silica-coated SPIONs, SPIONs coated with silica and functionalized with positively charged amino groups or negatively charged carboxyl groups (the primary size of all the surface-modified SPIONs was ~20 nm). Small unilamellar vesicles prepared with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine lipids and multilamellar vesicles prepared with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine lipids were encapsulated or incubated with the plain and surface-modified SPIONs to determine the fluidity and phase transition temperature of the bilayer lipids, respectively. Fluorescent anisotropy and differential scanning calorimetric measurements of the liposomes that were either encapsulated or incubated with the suspension of SPIONs did not show a significant difference in the lipid ordering and fluidity; though the encapsulated SPIONs showed a slightly increased effect on the fluidity of the model membranes in comparison with the incubated SPIONs. This indicates the low potential of the SPIONs to interact with the nontargeted cell membranes, which is a desirable factor for in vivo applications. Keywords: encapsulated SPIONs, incubated SPIONs, zwitterionic liposomes, membrane integrity, phase behavior
format	article
author	Santhosh PB Drašler B Drobne D Kreft ME Kralj S Makovec D Ulrih NP
author_facet	Santhosh PB Drašler B Drobne D Kreft ME Kralj S Makovec D Ulrih NP
author_sort	Santhosh PB
title	Effect of superparamagnetic iron oxide nanoparticles on fluidity and phase transition of phosphatidylcholine liposomal membranes
title_short	Effect of superparamagnetic iron oxide nanoparticles on fluidity and phase transition of phosphatidylcholine liposomal membranes
title_full	Effect of superparamagnetic iron oxide nanoparticles on fluidity and phase transition of phosphatidylcholine liposomal membranes
title_fullStr	Effect of superparamagnetic iron oxide nanoparticles on fluidity and phase transition of phosphatidylcholine liposomal membranes
title_full_unstemmed	Effect of superparamagnetic iron oxide nanoparticles on fluidity and phase transition of phosphatidylcholine liposomal membranes
title_sort	effect of superparamagnetic iron oxide nanoparticles on fluidity and phase transition of phosphatidylcholine liposomal membranes
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/8e7f3dcd2a824ad9bee113a038266754
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Effect of superparamagnetic iron oxide nanoparticles on fluidity and phase transition of phosphatidylcholine liposomal membranes

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