Development and Characterization of Alkaline Phosphatase-Positive Human Umbilical Cord Perivascular Cells

Human umbilical cord perivascular cells (HUCPVCs), harvested from human umbilical cord perivascular tissue, show potential for future use as an alternative to mesenchymal stromal cells. Here, we present the results for the characterization of the properties alkaline phosphatase-positive HUCPVCs (ALP...

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Autores principales:	Shun Nonoyama, Takeo Karakida, Risako Chiba-Ohkuma, Ryuji Yamamoto, Yuko Ujiie, Takatoshi Nagano, Yasuo Yamakoshi, Kazuhiro Gomi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	human umbilical cord perivascular cell alkaline phosphatase transforming growth factor-beta fibroblast myofibroblast Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8e86df278f7c4f2ab8b13bde6818e09c
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spelling	oai:doaj.org-article:8e86df278f7c4f2ab8b13bde6818e09c2021-11-25T17:10:23ZDevelopment and Characterization of Alkaline Phosphatase-Positive Human Umbilical Cord Perivascular Cells10.3390/cells101130112073-4409https://doaj.org/article/8e86df278f7c4f2ab8b13bde6818e09c2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3011https://doaj.org/toc/2073-4409Human umbilical cord perivascular cells (HUCPVCs), harvested from human umbilical cord perivascular tissue, show potential for future use as an alternative to mesenchymal stromal cells. Here, we present the results for the characterization of the properties alkaline phosphatase-positive HUCPVCs (ALP(+)-HUCPVCs). These ALP(+)-HUCPVCs were created from HUCPVCs in this study by culturing in the presence of activated vitamin D3, an inhibitor of bone morphogenetic protein signaling and transforming growth factor-beta1 (TGF-β1). The morphological characteristics, cell proliferation, gene expression, and mineralization-inducing ability of ALP(+)-HUCPVCs were investigated at the morphological, biological, and genetic levels. ALP(+)-HUCPVCs possess high ALP gene expression and activity in cells and a slow rate of cell growth. The morphology of ALP(+)-HUCPVCs is fibroblast-like, with an increase in actin filaments containing alpha-smooth muscle actin. In addition to ALP expression, the gene expression levels of type I collagen, osteopontin, elastin, fibrillin-1, and cluster of differentiation 90 are increased in ALP(+)-HUCPVCs. ALP(+)-HUCPVCs do not have the ability to induce mineralization nodules, which may be due to the restriction of phosphate uptake into matrix vesicles. Moreover, ALP(+)-HUCPVCs may produce anti-mineralization substances. We conclude that ALP(+)-HUCPVCs induced from HUCPVCs by a TGF-β1 stimulation possess myofibroblast-like properties that have little mineralization-inducing ability.Shun NonoyamaTakeo KarakidaRisako Chiba-OhkumaRyuji YamamotoYuko UjiieTakatoshi NaganoYasuo YamakoshiKazuhiro GomiMDPI AGarticlehuman umbilical cord perivascular cellalkaline phosphatasetransforming growth factor-betafibroblastmyofibroblastBiology (General)QH301-705.5ENCells, Vol 10, Iss 3011, p 3011 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	human umbilical cord perivascular cell alkaline phosphatase transforming growth factor-beta fibroblast myofibroblast Biology (General) QH301-705.5
spellingShingle	human umbilical cord perivascular cell alkaline phosphatase transforming growth factor-beta fibroblast myofibroblast Biology (General) QH301-705.5 Shun Nonoyama Takeo Karakida Risako Chiba-Ohkuma Ryuji Yamamoto Yuko Ujiie Takatoshi Nagano Yasuo Yamakoshi Kazuhiro Gomi Development and Characterization of Alkaline Phosphatase-Positive Human Umbilical Cord Perivascular Cells
description	Human umbilical cord perivascular cells (HUCPVCs), harvested from human umbilical cord perivascular tissue, show potential for future use as an alternative to mesenchymal stromal cells. Here, we present the results for the characterization of the properties alkaline phosphatase-positive HUCPVCs (ALP(+)-HUCPVCs). These ALP(+)-HUCPVCs were created from HUCPVCs in this study by culturing in the presence of activated vitamin D3, an inhibitor of bone morphogenetic protein signaling and transforming growth factor-beta1 (TGF-β1). The morphological characteristics, cell proliferation, gene expression, and mineralization-inducing ability of ALP(+)-HUCPVCs were investigated at the morphological, biological, and genetic levels. ALP(+)-HUCPVCs possess high ALP gene expression and activity in cells and a slow rate of cell growth. The morphology of ALP(+)-HUCPVCs is fibroblast-like, with an increase in actin filaments containing alpha-smooth muscle actin. In addition to ALP expression, the gene expression levels of type I collagen, osteopontin, elastin, fibrillin-1, and cluster of differentiation 90 are increased in ALP(+)-HUCPVCs. ALP(+)-HUCPVCs do not have the ability to induce mineralization nodules, which may be due to the restriction of phosphate uptake into matrix vesicles. Moreover, ALP(+)-HUCPVCs may produce anti-mineralization substances. We conclude that ALP(+)-HUCPVCs induced from HUCPVCs by a TGF-β1 stimulation possess myofibroblast-like properties that have little mineralization-inducing ability.
format	article
author	Shun Nonoyama Takeo Karakida Risako Chiba-Ohkuma Ryuji Yamamoto Yuko Ujiie Takatoshi Nagano Yasuo Yamakoshi Kazuhiro Gomi
author_facet	Shun Nonoyama Takeo Karakida Risako Chiba-Ohkuma Ryuji Yamamoto Yuko Ujiie Takatoshi Nagano Yasuo Yamakoshi Kazuhiro Gomi
author_sort	Shun Nonoyama
title	Development and Characterization of Alkaline Phosphatase-Positive Human Umbilical Cord Perivascular Cells
title_short	Development and Characterization of Alkaline Phosphatase-Positive Human Umbilical Cord Perivascular Cells
title_full	Development and Characterization of Alkaline Phosphatase-Positive Human Umbilical Cord Perivascular Cells
title_fullStr	Development and Characterization of Alkaline Phosphatase-Positive Human Umbilical Cord Perivascular Cells
title_full_unstemmed	Development and Characterization of Alkaline Phosphatase-Positive Human Umbilical Cord Perivascular Cells
title_sort	development and characterization of alkaline phosphatase-positive human umbilical cord perivascular cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8e86df278f7c4f2ab8b13bde6818e09c
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Development and Characterization of Alkaline Phosphatase-Positive Human Umbilical Cord Perivascular Cells

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