The effect of hydrolysis duration on the antibacterial activity of swamp eel head protein hydrolysate produced by papain against histamine-producing bacteria

Swamp eel (Monopterus albus) processing produced byproducts such as heads that possess a high protein, carbohydrate, lipid, and energies content. Swamp eel heads were hydrolyzed (SEHPH) and tested for their antibacterial activity against histamine-producing bacteria (HPB) to explore the potency. The...

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Autores principales:	Hafifani Witya, Puspita Indun D., Putra Masagus M. P.
Formato:	article
Lenguaje:	EN FR
Publicado:	EDP Sciences 2021
Materias:	Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/8e87c8817fea4dfc8a9f78094d4a723e
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spelling	oai:doaj.org-article:8e87c8817fea4dfc8a9f78094d4a723e2021-11-12T11:44:43ZThe effect of hydrolysis duration on the antibacterial activity of swamp eel head protein hydrolysate produced by papain against histamine-producing bacteria2267-124210.1051/e3sconf/202132204005https://doaj.org/article/8e87c8817fea4dfc8a9f78094d4a723e2021-01-01T00:00:00Zhttps://www.e3s-conferences.org/articles/e3sconf/pdf/2021/98/e3sconf_isffs2021_04005.pdfhttps://doaj.org/toc/2267-1242Swamp eel (Monopterus albus) processing produced byproducts such as heads that possess a high protein, carbohydrate, lipid, and energies content. Swamp eel heads were hydrolyzed (SEHPH) and tested for their antibacterial activity against histamine-producing bacteria (HPB) to explore the potency. The hydrolysis was conducted by a commercial papain enzyme PAYA (concentration 4%) with various hydrolysis times (0, 60, 120, and 180 minutes) at 60o C with pH 5. The results indicated that increasing hydrolysis time increased solubility and the degree of hydrolysis. The inhibitor concentration 50 test using microdilution of SEHPH inhibited 50% against three HPB named Citrobacter sp. (CK1), Klebsiella sp. (CK13), and Morganella morganii (TK7) at 20 mg/ml. The optimum antibacterial activity test by disk diffusion method was formed by each minute of hydrolysis with the best inhibition zone to Klebsiella sp. (CK13). The macrodillution method showed the highest inhibition was produced by 180 hydrolyses with 45% on CK1, 54% on CK13, and 48% on TK7. These results indicated that SEHPH has potency as an antibacterial agent to reduce histamine-producing bacteria.Hafifani WityaPuspita Indun D.Putra Masagus M. P.EDP SciencesarticleEnvironmental sciencesGE1-350ENFRE3S Web of Conferences, Vol 322, p 04005 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR
topic	Environmental sciences GE1-350
spellingShingle	Environmental sciences GE1-350 Hafifani Witya Puspita Indun D. Putra Masagus M. P. The effect of hydrolysis duration on the antibacterial activity of swamp eel head protein hydrolysate produced by papain against histamine-producing bacteria
description	Swamp eel (Monopterus albus) processing produced byproducts such as heads that possess a high protein, carbohydrate, lipid, and energies content. Swamp eel heads were hydrolyzed (SEHPH) and tested for their antibacterial activity against histamine-producing bacteria (HPB) to explore the potency. The hydrolysis was conducted by a commercial papain enzyme PAYA (concentration 4%) with various hydrolysis times (0, 60, 120, and 180 minutes) at 60o C with pH 5. The results indicated that increasing hydrolysis time increased solubility and the degree of hydrolysis. The inhibitor concentration 50 test using microdilution of SEHPH inhibited 50% against three HPB named Citrobacter sp. (CK1), Klebsiella sp. (CK13), and Morganella morganii (TK7) at 20 mg/ml. The optimum antibacterial activity test by disk diffusion method was formed by each minute of hydrolysis with the best inhibition zone to Klebsiella sp. (CK13). The macrodillution method showed the highest inhibition was produced by 180 hydrolyses with 45% on CK1, 54% on CK13, and 48% on TK7. These results indicated that SEHPH has potency as an antibacterial agent to reduce histamine-producing bacteria.
format	article
author	Hafifani Witya Puspita Indun D. Putra Masagus M. P.
author_facet	Hafifani Witya Puspita Indun D. Putra Masagus M. P.
author_sort	Hafifani Witya
title	The effect of hydrolysis duration on the antibacterial activity of swamp eel head protein hydrolysate produced by papain against histamine-producing bacteria
title_short	The effect of hydrolysis duration on the antibacterial activity of swamp eel head protein hydrolysate produced by papain against histamine-producing bacteria
title_full	The effect of hydrolysis duration on the antibacterial activity of swamp eel head protein hydrolysate produced by papain against histamine-producing bacteria
title_fullStr	The effect of hydrolysis duration on the antibacterial activity of swamp eel head protein hydrolysate produced by papain against histamine-producing bacteria
title_full_unstemmed	The effect of hydrolysis duration on the antibacterial activity of swamp eel head protein hydrolysate produced by papain against histamine-producing bacteria
title_sort	effect of hydrolysis duration on the antibacterial activity of swamp eel head protein hydrolysate produced by papain against histamine-producing bacteria
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/8e87c8817fea4dfc8a9f78094d4a723e
work_keys_str_mv	AT hafifaniwitya theeffectofhydrolysisdurationontheantibacterialactivityofswampeelheadproteinhydrolysateproducedbypapainagainsthistamineproducingbacteria AT puspitaindund theeffectofhydrolysisdurationontheantibacterialactivityofswampeelheadproteinhydrolysateproducedbypapainagainsthistamineproducingbacteria AT putramasagusmp theeffectofhydrolysisdurationontheantibacterialactivityofswampeelheadproteinhydrolysateproducedbypapainagainsthistamineproducingbacteria AT hafifaniwitya effectofhydrolysisdurationontheantibacterialactivityofswampeelheadproteinhydrolysateproducedbypapainagainsthistamineproducingbacteria AT puspitaindund effectofhydrolysisdurationontheantibacterialactivityofswampeelheadproteinhydrolysateproducedbypapainagainsthistamineproducingbacteria AT putramasagusmp effectofhydrolysisdurationontheantibacterialactivityofswampeelheadproteinhydrolysateproducedbypapainagainsthistamineproducingbacteria
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The effect of hydrolysis duration on the antibacterial activity of swamp eel head protein hydrolysate produced by papain against histamine-producing bacteria

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