Comparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection

Abstract Asparagus kiusianus, an important wild relative of cultivated asparagus (A. officinalis), exhibits resistance to stem blight disease caused by Phomopsis asparagi. However, the mechanisms underlying this resistance are not understood and no transcriptomic or genetic resources are available f...

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Autores principales:	Mostafa Abdelrahman, Naoyuki Suzumura, Mai Mitoma, Satoshi Matsuo, Takao Ikeuchi, Mitsutaka Mori, Kyoko Murakami, Yukio Ozaki, Masaru Matsumoto, Atsuko Uragami, Akira Kanno
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8e8b1b2bc55a4d0e976070a930f7c674
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spelling	oai:doaj.org-article:8e8b1b2bc55a4d0e976070a930f7c6742021-12-02T16:07:56ZComparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection10.1038/s41598-017-02566-72045-2322https://doaj.org/article/8e8b1b2bc55a4d0e976070a930f7c6742017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02566-7https://doaj.org/toc/2045-2322Abstract Asparagus kiusianus, an important wild relative of cultivated asparagus (A. officinalis), exhibits resistance to stem blight disease caused by Phomopsis asparagi. However, the mechanisms underlying this resistance are not understood and no transcriptomic or genetic resources are available for this species. De novo transcriptome sequencing of A. officinalis and A. kiusianus stems was performed 24 h after inoculation with P. asparagi. In total, 35,259 and 36,321 transcripts were annotated in A. officinalis and A. kiusianus, respectively. 1,027 up-regulated and 752 down-regulated transcripts were differentially expressed in the two Asparagus species. RNA sequencing data were validated using quantitative real-time reverse transcription PCR. Several defense-related genes including peroxidase 4, cationic peroxidase SPC4-like, pathogenesis-related protein-1-like, and jasmonic acid biosynthesis and signaling-related genes including phospholipase D alpha 1, 12-oxophytodienoate reductase and jasmonate-induced protein 23 KD were up-regulated in A. kiusianus relative to A. officinalis. In addition, infected A. kiusianuns exhibited a substantial increase in jasmonic acid and methyl jasmonate relative to A. officinalis. Peroxidase activity was significantly elevated in infected A. kiusianus compared with infected A. officinalis. Our transcriptomic database provides a resource for identifying novel genes and molecular markers-associated with Phomopsis disease resistance and will facilitate breeding and improvement of cultivated asparagus varieties.Mostafa AbdelrahmanNaoyuki SuzumuraMai MitomaSatoshi MatsuoTakao IkeuchiMitsutaka MoriKyoko MurakamiYukio OzakiMasaru MatsumotoAtsuko UragamiAkira KannoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mostafa Abdelrahman Naoyuki Suzumura Mai Mitoma Satoshi Matsuo Takao Ikeuchi Mitsutaka Mori Kyoko Murakami Yukio Ozaki Masaru Matsumoto Atsuko Uragami Akira Kanno Comparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection
description	Abstract Asparagus kiusianus, an important wild relative of cultivated asparagus (A. officinalis), exhibits resistance to stem blight disease caused by Phomopsis asparagi. However, the mechanisms underlying this resistance are not understood and no transcriptomic or genetic resources are available for this species. De novo transcriptome sequencing of A. officinalis and A. kiusianus stems was performed 24 h after inoculation with P. asparagi. In total, 35,259 and 36,321 transcripts were annotated in A. officinalis and A. kiusianus, respectively. 1,027 up-regulated and 752 down-regulated transcripts were differentially expressed in the two Asparagus species. RNA sequencing data were validated using quantitative real-time reverse transcription PCR. Several defense-related genes including peroxidase 4, cationic peroxidase SPC4-like, pathogenesis-related protein-1-like, and jasmonic acid biosynthesis and signaling-related genes including phospholipase D alpha 1, 12-oxophytodienoate reductase and jasmonate-induced protein 23 KD were up-regulated in A. kiusianus relative to A. officinalis. In addition, infected A. kiusianuns exhibited a substantial increase in jasmonic acid and methyl jasmonate relative to A. officinalis. Peroxidase activity was significantly elevated in infected A. kiusianus compared with infected A. officinalis. Our transcriptomic database provides a resource for identifying novel genes and molecular markers-associated with Phomopsis disease resistance and will facilitate breeding and improvement of cultivated asparagus varieties.
format	article
author	Mostafa Abdelrahman Naoyuki Suzumura Mai Mitoma Satoshi Matsuo Takao Ikeuchi Mitsutaka Mori Kyoko Murakami Yukio Ozaki Masaru Matsumoto Atsuko Uragami Akira Kanno
author_facet	Mostafa Abdelrahman Naoyuki Suzumura Mai Mitoma Satoshi Matsuo Takao Ikeuchi Mitsutaka Mori Kyoko Murakami Yukio Ozaki Masaru Matsumoto Atsuko Uragami Akira Kanno
author_sort	Mostafa Abdelrahman
title	Comparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection
title_short	Comparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection
title_full	Comparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection
title_fullStr	Comparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection
title_full_unstemmed	Comparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection
title_sort	comparative de novo transcriptome profiles in asparagus officinalis and a. kiusianus during the early stage of phomopsis asparagi infection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8e8b1b2bc55a4d0e976070a930f7c674
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Comparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection

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