The effect of chemical and structural modifiers on the haemostatic process and cytotoxicity of the beta-chitin patch

Abstract Beta-chitin patch has previously been proven to be an effective haemostat, but whether modifying the patch affects its efficacy and safety, remains unanswered. In this study, the patch was modified using polyethylene oxide, Pluronic-F127, calcium, increased thickness or polyphosphate, and t...

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Autores principales:	Ahad Sabab, Sha Liu, Shari Javadiyan, C. John McAdam, Lyall R. Hanton, Alistair Jukes, Sarah Vreugde, Peter-John Wormald
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8e8cc96d708a4eb0af32510973ec0355
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spelling	oai:doaj.org-article:8e8cc96d708a4eb0af32510973ec03552021-12-02T18:33:46ZThe effect of chemical and structural modifiers on the haemostatic process and cytotoxicity of the beta-chitin patch10.1038/s41598-021-97781-82045-2322https://doaj.org/article/8e8cc96d708a4eb0af32510973ec03552021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97781-8https://doaj.org/toc/2045-2322Abstract Beta-chitin patch has previously been proven to be an effective haemostat, but whether modifying the patch affects its efficacy and safety, remains unanswered. In this study, the patch was modified using polyethylene oxide, Pluronic-F127, calcium, increased thickness or polyphosphate, and their effect on the process of haemostasis and cytotoxicity was tested and compared with standard-of-care, Surgicel and FloSeal. Whole blood collected from volunteers was applied to the patches to test their whole blood clotting and thrombin generation capacities, whilst platelet isolates were used to test their platelet aggregation ability. The fluid absorption capacity of the patches was tested using simulated body fluid. Cytotoxicity of the patches was tested using AlamarBlue assays and PC12 cells and the results were compared with the standard-of-care. In this study, beta-chitin patch modifications failed to improve its whole blood clotting, platelet aggregation and thrombin generation capacity. Compared to non-modified patch, modifications with polyethylene oxide or calcium reduced platelet aggregation and thrombin generation capacity, while increasing the thickness or adding polyphosphate decreased platelet aggregation capacity. The cytotoxicity assays demonstrated that the beta-chitin patches were non-toxic to cells. In vivo research is required to evaluate the safety and efficacy of the beta-chitin patches in a clinical setting.Ahad SababSha LiuShari JavadiyanC. John McAdamLyall R. HantonAlistair JukesSarah VreugdePeter-John WormaldNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ahad Sabab Sha Liu Shari Javadiyan C. John McAdam Lyall R. Hanton Alistair Jukes Sarah Vreugde Peter-John Wormald The effect of chemical and structural modifiers on the haemostatic process and cytotoxicity of the beta-chitin patch
description	Abstract Beta-chitin patch has previously been proven to be an effective haemostat, but whether modifying the patch affects its efficacy and safety, remains unanswered. In this study, the patch was modified using polyethylene oxide, Pluronic-F127, calcium, increased thickness or polyphosphate, and their effect on the process of haemostasis and cytotoxicity was tested and compared with standard-of-care, Surgicel and FloSeal. Whole blood collected from volunteers was applied to the patches to test their whole blood clotting and thrombin generation capacities, whilst platelet isolates were used to test their platelet aggregation ability. The fluid absorption capacity of the patches was tested using simulated body fluid. Cytotoxicity of the patches was tested using AlamarBlue assays and PC12 cells and the results were compared with the standard-of-care. In this study, beta-chitin patch modifications failed to improve its whole blood clotting, platelet aggregation and thrombin generation capacity. Compared to non-modified patch, modifications with polyethylene oxide or calcium reduced platelet aggregation and thrombin generation capacity, while increasing the thickness or adding polyphosphate decreased platelet aggregation capacity. The cytotoxicity assays demonstrated that the beta-chitin patches were non-toxic to cells. In vivo research is required to evaluate the safety and efficacy of the beta-chitin patches in a clinical setting.
format	article
author	Ahad Sabab Sha Liu Shari Javadiyan C. John McAdam Lyall R. Hanton Alistair Jukes Sarah Vreugde Peter-John Wormald
author_facet	Ahad Sabab Sha Liu Shari Javadiyan C. John McAdam Lyall R. Hanton Alistair Jukes Sarah Vreugde Peter-John Wormald
author_sort	Ahad Sabab
title	The effect of chemical and structural modifiers on the haemostatic process and cytotoxicity of the beta-chitin patch
title_short	The effect of chemical and structural modifiers on the haemostatic process and cytotoxicity of the beta-chitin patch
title_full	The effect of chemical and structural modifiers on the haemostatic process and cytotoxicity of the beta-chitin patch
title_fullStr	The effect of chemical and structural modifiers on the haemostatic process and cytotoxicity of the beta-chitin patch
title_full_unstemmed	The effect of chemical and structural modifiers on the haemostatic process and cytotoxicity of the beta-chitin patch
title_sort	effect of chemical and structural modifiers on the haemostatic process and cytotoxicity of the beta-chitin patch
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8e8cc96d708a4eb0af32510973ec0355
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The effect of chemical and structural modifiers on the haemostatic process and cytotoxicity of the beta-chitin patch

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