Comparative 2D-DIGE proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield.
Mammary gland is made up of a branching network of ducts that end with alveoli which surrounds the lumen. These alveolar mammary epithelial cells (MEC) reflect the milk producing ability of farm animals. In this study, we have used 2D-DIGE and mass spectrometry to identify the protein changes in MEC...
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2014
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oai:doaj.org-article:8e9293a1ce6b4e28ad103d433f392bde2021-11-25T06:05:15ZComparative 2D-DIGE proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield.1932-620310.1371/journal.pone.0102515https://doaj.org/article/8e9293a1ce6b4e28ad103d433f392bde2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25111801/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Mammary gland is made up of a branching network of ducts that end with alveoli which surrounds the lumen. These alveolar mammary epithelial cells (MEC) reflect the milk producing ability of farm animals. In this study, we have used 2D-DIGE and mass spectrometry to identify the protein changes in MEC during immediate early, peak and late stages of lactation and also compared differentially expressed proteins in MEC isolated from milk of high and low milk producing cows. We have identified 41 differentially expressed proteins during lactation stages and 22 proteins in high and low milk yielding cows. Bioinformatics analysis showed that a majority of the differentially expressed proteins are associated in metabolic process, catalytic and binding activity. The differentially expressed proteins were mapped to the available biological pathways and networks involved in lactation. The proteins up-regulated during late stage of lactation are associated with NF-κB stress induced signaling pathways and whereas Akt, PI3K and p38/MAPK signaling pathways are associated with high milk production mediated through insulin hormone signaling.Jagadeesh JanjanamSurender SinghManoj K JenaNishant VarshneySrujana KolaSudarshan KumarJai K KaushikSunita GroverAjay K DangManishi MukeshB S PrakashAshok K MohantyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e102515 (2014) |
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DOAJ |
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Medicine R Science Q |
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Medicine R Science Q Jagadeesh Janjanam Surender Singh Manoj K Jena Nishant Varshney Srujana Kola Sudarshan Kumar Jai K Kaushik Sunita Grover Ajay K Dang Manishi Mukesh B S Prakash Ashok K Mohanty Comparative 2D-DIGE proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield. |
| description |
Mammary gland is made up of a branching network of ducts that end with alveoli which surrounds the lumen. These alveolar mammary epithelial cells (MEC) reflect the milk producing ability of farm animals. In this study, we have used 2D-DIGE and mass spectrometry to identify the protein changes in MEC during immediate early, peak and late stages of lactation and also compared differentially expressed proteins in MEC isolated from milk of high and low milk producing cows. We have identified 41 differentially expressed proteins during lactation stages and 22 proteins in high and low milk yielding cows. Bioinformatics analysis showed that a majority of the differentially expressed proteins are associated in metabolic process, catalytic and binding activity. The differentially expressed proteins were mapped to the available biological pathways and networks involved in lactation. The proteins up-regulated during late stage of lactation are associated with NF-κB stress induced signaling pathways and whereas Akt, PI3K and p38/MAPK signaling pathways are associated with high milk production mediated through insulin hormone signaling. |
| format |
article |
| author |
Jagadeesh Janjanam Surender Singh Manoj K Jena Nishant Varshney Srujana Kola Sudarshan Kumar Jai K Kaushik Sunita Grover Ajay K Dang Manishi Mukesh B S Prakash Ashok K Mohanty |
| author_facet |
Jagadeesh Janjanam Surender Singh Manoj K Jena Nishant Varshney Srujana Kola Sudarshan Kumar Jai K Kaushik Sunita Grover Ajay K Dang Manishi Mukesh B S Prakash Ashok K Mohanty |
| author_sort |
Jagadeesh Janjanam |
| title |
Comparative 2D-DIGE proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield. |
| title_short |
Comparative 2D-DIGE proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield. |
| title_full |
Comparative 2D-DIGE proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield. |
| title_fullStr |
Comparative 2D-DIGE proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield. |
| title_full_unstemmed |
Comparative 2D-DIGE proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield. |
| title_sort |
comparative 2d-dige proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/8e9293a1ce6b4e28ad103d433f392bde |
| work_keys_str_mv |
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1718414234651459584 |