Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia

Putra DE, Sumadi, Kanazawa T, Hartatik T. 2016. Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia. Biodiversitas 17: 711-715. This study aimed to determine gene polymorphism of growth hormone of domestic cattl...

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Autores principales:	DINO EKA PUTRA, SUMADI SUMADI, TAKUYA KANAZAWA, TETY HARTATIK
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2016
Materias:	domestic cattle, growth hormone gene, polymorphism Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8e935c01985c4e81add98b52139622a7
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spelling	oai:doaj.org-article:8e935c01985c4e81add98b52139622a72021-11-14T02:27:09ZShort Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia1412-033X2085-472210.13057/biodiv/d170246https://doaj.org/article/8e935c01985c4e81add98b52139622a72016-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/349https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Putra DE, Sumadi, Kanazawa T, Hartatik T. 2016. Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia. Biodiversitas 17: 711-715. This study aimed to determine gene polymorphism of growth hormone of domestic cattle in Pesisir Selatan District of West Sumatra. The research was conducted at Laboratory of Animal Genetics and Breeding Faculty of Animal Science, UGM from August 2013 to January 2014. Blood samples were collected from 66 individuals consist of 15 Pesisir cattle, 15 SimPes cattle, 15 SimPO cattle, 15 Bali cattle and 6 PO cattle. DNA was extracted from each blood samples after SDS-proteinase K digestion, and used for PCR-amplification for a region of growth hormone gene (211 bp), and then the PCR products were analyzed for restriction fragment length polymorphism (RFLP) using AluI restriction enzyme. The results showed that GH gene of Pesisir, PO and Bali cattle are monomorphic, which frequencies of L allele was 1.00 and V allele was 0.000 while these LL genotype was 1.000. Frequency of L and V alleles in SimPO and SimPes cattle were 0.634, 0.366 and 0.700, 0.300, respectively. SimPO and SimPes cattle were polymorphic, LL and LV of SimPO cattle was 0.733 and 0.267 as well as SimPes cattle which LL and LV was 0.600 and 0.400, respectively. The correlation between genotype and the performance (body weight and body size) was not significant. The present study indicates that polymorphism of growth hormone gene in AluI site could not yet be used as a molecular marker for body weight and body size of beef cattle.DINO EKA PUTRASUMADI SUMADITAKUYA KANAZAWATETY HARTATIKMBI & UNS Soloarticledomestic cattle, growth hormone gene, polymorphismBiology (General)QH301-705.5ENBiodiversitas, Vol 17, Iss 2 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	domestic cattle, growth hormone gene, polymorphism Biology (General) QH301-705.5
spellingShingle	domestic cattle, growth hormone gene, polymorphism Biology (General) QH301-705.5 DINO EKA PUTRA SUMADI SUMADI TAKUYA KANAZAWA TETY HARTATIK Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia
description	Putra DE, Sumadi, Kanazawa T, Hartatik T. 2016. Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia. Biodiversitas 17: 711-715. This study aimed to determine gene polymorphism of growth hormone of domestic cattle in Pesisir Selatan District of West Sumatra. The research was conducted at Laboratory of Animal Genetics and Breeding Faculty of Animal Science, UGM from August 2013 to January 2014. Blood samples were collected from 66 individuals consist of 15 Pesisir cattle, 15 SimPes cattle, 15 SimPO cattle, 15 Bali cattle and 6 PO cattle. DNA was extracted from each blood samples after SDS-proteinase K digestion, and used for PCR-amplification for a region of growth hormone gene (211 bp), and then the PCR products were analyzed for restriction fragment length polymorphism (RFLP) using AluI restriction enzyme. The results showed that GH gene of Pesisir, PO and Bali cattle are monomorphic, which frequencies of L allele was 1.00 and V allele was 0.000 while these LL genotype was 1.000. Frequency of L and V alleles in SimPO and SimPes cattle were 0.634, 0.366 and 0.700, 0.300, respectively. SimPO and SimPes cattle were polymorphic, LL and LV of SimPO cattle was 0.733 and 0.267 as well as SimPes cattle which LL and LV was 0.600 and 0.400, respectively. The correlation between genotype and the performance (body weight and body size) was not significant. The present study indicates that polymorphism of growth hormone gene in AluI site could not yet be used as a molecular marker for body weight and body size of beef cattle.
format	article
author	DINO EKA PUTRA SUMADI SUMADI TAKUYA KANAZAWA TETY HARTATIK
author_facet	DINO EKA PUTRA SUMADI SUMADI TAKUYA KANAZAWA TETY HARTATIK
author_sort	DINO EKA PUTRA
title	Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia
title_short	Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia
title_full	Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia
title_fullStr	Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia
title_full_unstemmed	Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia
title_sort	short communication: identification of growth hormone gene polymorphism for beef cattle in pesisir selatan district, west sumatra, indonesia
publisher	MBI & UNS Solo
publishDate	2016
url	https://doaj.org/article/8e935c01985c4e81add98b52139622a7
work_keys_str_mv	AT dinoekaputra shortcommunicationidentificationofgrowthhormonegenepolymorphismforbeefcattleinpesisirselatandistrictwestsumatraindonesia AT sumadisumadi shortcommunicationidentificationofgrowthhormonegenepolymorphismforbeefcattleinpesisirselatandistrictwestsumatraindonesia AT takuyakanazawa shortcommunicationidentificationofgrowthhormonegenepolymorphismforbeefcattleinpesisirselatandistrictwestsumatraindonesia AT tetyhartatik shortcommunicationidentificationofgrowthhormonegenepolymorphismforbeefcattleinpesisirselatandistrictwestsumatraindonesia
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Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia

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