Photosynthetic electron transport system promotes synthesis of Au-nanoparticles.

In this communication, a novel, green, efficient and economically viable light mediated protocol for generation of Au-nanoparticles using most vital organelle, chloroplasts, of the plant system is portrayed. Thylakoids/chloroplasts isolated from Potamogeton nodosus (an aquatic plant) and Spinacia ol...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nisha Shabnam, P Pardha-Saradhi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8ea27b80eb7e4fb7a949c737d9688cdb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8ea27b80eb7e4fb7a949c737d9688cdb
record_format	dspace
spelling	oai:doaj.org-article:8ea27b80eb7e4fb7a949c737d9688cdb2021-11-18T08:58:51ZPhotosynthetic electron transport system promotes synthesis of Au-nanoparticles.1932-620310.1371/journal.pone.0071123https://doaj.org/article/8ea27b80eb7e4fb7a949c737d9688cdb2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23976990/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In this communication, a novel, green, efficient and economically viable light mediated protocol for generation of Au-nanoparticles using most vital organelle, chloroplasts, of the plant system is portrayed. Thylakoids/chloroplasts isolated from Potamogeton nodosus (an aquatic plant) and Spinacia oleracea (a terrestrial plant) turned Au³⁺ solutions purple in presence of light of 600 µmol m⁻² s⁻¹ photon flux density (PFD) and the purple coloration intensified with time. UV-Vis spectra of these purple colored solutions showed absorption peak at ∼545 nm which is known to arise due to surface plasmon oscillations specific to Au-nanoparticles. However, thylakoids/chloroplasts did not alter color of Au³⁺ solutions in dark. These results clearly demonstrated that photosynthetic electron transport can reduce Au³⁺ to Au⁰ which nucleate to form Au-nanoparticles in presence of light. Transmission electron microscopic studies revealed that Au-nanoparticles generated by light driven photosynthetic electron transport system of thylakoids/chloroplasts were in range of 5-20 nm. Selected area electron diffraction and powder X-ray diffraction indicated crystalline nature of these nanoparticles. Energy dispersive X-ray confirmed that these nanoparticles were composed of Au. To confirm the potential of light driven photosynthetic electron transport in generation of Au-nanoparticles, thylakoids/chloroplasts were tested for their efficacy to generate Au-nanoparticles in presence of light of PFD ranging from 60 to 600 µmol m⁻² s⁻¹. The capacity of thylakoids/chloroplasts to generate Au-nanoparticles increased remarkably with increase in PFD, which further clearly demonstrated potential of light driven photosynthetic electron transport in reduction of Au³⁺ to Au⁰ to form nanoparticles. The light driven donation of electrons to metal ions by thylakoids/chloroplasts can be exploited for large scale production of nanoparticles.Nisha ShabnamP Pardha-SaradhiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e71123 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nisha Shabnam P Pardha-Saradhi Photosynthetic electron transport system promotes synthesis of Au-nanoparticles.
description	In this communication, a novel, green, efficient and economically viable light mediated protocol for generation of Au-nanoparticles using most vital organelle, chloroplasts, of the plant system is portrayed. Thylakoids/chloroplasts isolated from Potamogeton nodosus (an aquatic plant) and Spinacia oleracea (a terrestrial plant) turned Au³⁺ solutions purple in presence of light of 600 µmol m⁻² s⁻¹ photon flux density (PFD) and the purple coloration intensified with time. UV-Vis spectra of these purple colored solutions showed absorption peak at ∼545 nm which is known to arise due to surface plasmon oscillations specific to Au-nanoparticles. However, thylakoids/chloroplasts did not alter color of Au³⁺ solutions in dark. These results clearly demonstrated that photosynthetic electron transport can reduce Au³⁺ to Au⁰ which nucleate to form Au-nanoparticles in presence of light. Transmission electron microscopic studies revealed that Au-nanoparticles generated by light driven photosynthetic electron transport system of thylakoids/chloroplasts were in range of 5-20 nm. Selected area electron diffraction and powder X-ray diffraction indicated crystalline nature of these nanoparticles. Energy dispersive X-ray confirmed that these nanoparticles were composed of Au. To confirm the potential of light driven photosynthetic electron transport in generation of Au-nanoparticles, thylakoids/chloroplasts were tested for their efficacy to generate Au-nanoparticles in presence of light of PFD ranging from 60 to 600 µmol m⁻² s⁻¹. The capacity of thylakoids/chloroplasts to generate Au-nanoparticles increased remarkably with increase in PFD, which further clearly demonstrated potential of light driven photosynthetic electron transport in reduction of Au³⁺ to Au⁰ to form nanoparticles. The light driven donation of electrons to metal ions by thylakoids/chloroplasts can be exploited for large scale production of nanoparticles.
format	article
author	Nisha Shabnam P Pardha-Saradhi
author_facet	Nisha Shabnam P Pardha-Saradhi
author_sort	Nisha Shabnam
title	Photosynthetic electron transport system promotes synthesis of Au-nanoparticles.
title_short	Photosynthetic electron transport system promotes synthesis of Au-nanoparticles.
title_full	Photosynthetic electron transport system promotes synthesis of Au-nanoparticles.
title_fullStr	Photosynthetic electron transport system promotes synthesis of Au-nanoparticles.
title_full_unstemmed	Photosynthetic electron transport system promotes synthesis of Au-nanoparticles.
title_sort	photosynthetic electron transport system promotes synthesis of au-nanoparticles.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/8ea27b80eb7e4fb7a949c737d9688cdb
work_keys_str_mv	AT nishashabnam photosyntheticelectrontransportsystempromotessynthesisofaunanoparticles AT ppardhasaradhi photosyntheticelectrontransportsystempromotessynthesisofaunanoparticles
_version_	1718421095888977920

Photosynthetic electron transport system promotes synthesis of Au-nanoparticles.

Ejemplares similares