ZOLA-3D allows flexible 3D localization microscopy over an adjustable axial range

3D single-molecule localization is limited in depth and often requires using a wide range of point spread functions (PSFs). Here the authors present an optical solution featuring a deformable mirror to generate different PSFs and easy-to-use software for super-resolution imaging up to 5 µm deep.

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Detalles Bibliográficos
Autores principales: Andrey Aristov, Benoit Lelandais, Elena Rensen, Christophe Zimmer
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
Materias:
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Acceso en línea:https://doaj.org/article/8eb342d6af1b4fd785f67e8b1614fb3e
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Descripción
Sumario:3D single-molecule localization is limited in depth and often requires using a wide range of point spread functions (PSFs). Here the authors present an optical solution featuring a deformable mirror to generate different PSFs and easy-to-use software for super-resolution imaging up to 5 µm deep.