Age related extracellular matrix and interstitial cell phenotype in pulmonary valves

Abstract Heart valve disease is a common manifestation of cardiovascular disease and is a significant cause of cardiovascular morbidity and mortality worldwide. The pulmonary valve (PV) is of primary concern because of its involvement in common congenital heart defects, and the PV is usually the sit...

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Autores principales:	Shaohua Wu, Vikas Kumar, Peng Xiao, Mitchell Kuss, Jung Yul Lim, Chittibabu Guda, Jonathan Butcher, Bin Duan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8eb60de4a12b4d4e81af67d3af811050
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spelling	oai:doaj.org-article:8eb60de4a12b4d4e81af67d3af8110502021-12-02T15:10:18ZAge related extracellular matrix and interstitial cell phenotype in pulmonary valves10.1038/s41598-020-78507-82045-2322https://doaj.org/article/8eb60de4a12b4d4e81af67d3af8110502020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78507-8https://doaj.org/toc/2045-2322Abstract Heart valve disease is a common manifestation of cardiovascular disease and is a significant cause of cardiovascular morbidity and mortality worldwide. The pulmonary valve (PV) is of primary concern because of its involvement in common congenital heart defects, and the PV is usually the site for prosthetic replacement following a Ross operation. Although effects of age on valve matrix components and mechanical properties for aortic and mitral valves have been studied, very little is known about the age-related alterations that occur in the PV. In this study, we isolated PV leaflets from porcine hearts in different age groups (~ 4–6 months, denoted as young versus ~ 2 years, denoted as adult) and studied the effects of age on PV leaflet thickness, extracellular matrix components, and mechanical properties. We also conducted proteomics and RNA sequencing to investigate the global changes of PV leaflets and passage zero PV interstitial cells in their protein and gene levels. We found that the size, thickness, elastic modulus, and ultimate stress in both the radial and circumferential directions and the collagen of PV leaflets increased from young to adult age, while the ultimate strain and amount of glycosaminoglycans decreased when age increased. Young and adult PV had both similar and distinct protein and gene expression patterns that are related to their inherent physiological properties. These findings are important for us to better understand the physiological microenvironments of PV leaflet and valve cells for correctively engineering age-specific heart valve tissues.Shaohua WuVikas KumarPeng XiaoMitchell KussJung Yul LimChittibabu GudaJonathan ButcherBin DuanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shaohua Wu Vikas Kumar Peng Xiao Mitchell Kuss Jung Yul Lim Chittibabu Guda Jonathan Butcher Bin Duan Age related extracellular matrix and interstitial cell phenotype in pulmonary valves
description	Abstract Heart valve disease is a common manifestation of cardiovascular disease and is a significant cause of cardiovascular morbidity and mortality worldwide. The pulmonary valve (PV) is of primary concern because of its involvement in common congenital heart defects, and the PV is usually the site for prosthetic replacement following a Ross operation. Although effects of age on valve matrix components and mechanical properties for aortic and mitral valves have been studied, very little is known about the age-related alterations that occur in the PV. In this study, we isolated PV leaflets from porcine hearts in different age groups (~ 4–6 months, denoted as young versus ~ 2 years, denoted as adult) and studied the effects of age on PV leaflet thickness, extracellular matrix components, and mechanical properties. We also conducted proteomics and RNA sequencing to investigate the global changes of PV leaflets and passage zero PV interstitial cells in their protein and gene levels. We found that the size, thickness, elastic modulus, and ultimate stress in both the radial and circumferential directions and the collagen of PV leaflets increased from young to adult age, while the ultimate strain and amount of glycosaminoglycans decreased when age increased. Young and adult PV had both similar and distinct protein and gene expression patterns that are related to their inherent physiological properties. These findings are important for us to better understand the physiological microenvironments of PV leaflet and valve cells for correctively engineering age-specific heart valve tissues.
format	article
author	Shaohua Wu Vikas Kumar Peng Xiao Mitchell Kuss Jung Yul Lim Chittibabu Guda Jonathan Butcher Bin Duan
author_facet	Shaohua Wu Vikas Kumar Peng Xiao Mitchell Kuss Jung Yul Lim Chittibabu Guda Jonathan Butcher Bin Duan
author_sort	Shaohua Wu
title	Age related extracellular matrix and interstitial cell phenotype in pulmonary valves
title_short	Age related extracellular matrix and interstitial cell phenotype in pulmonary valves
title_full	Age related extracellular matrix and interstitial cell phenotype in pulmonary valves
title_fullStr	Age related extracellular matrix and interstitial cell phenotype in pulmonary valves
title_full_unstemmed	Age related extracellular matrix and interstitial cell phenotype in pulmonary valves
title_sort	age related extracellular matrix and interstitial cell phenotype in pulmonary valves
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/8eb60de4a12b4d4e81af67d3af811050
work_keys_str_mv	AT shaohuawu agerelatedextracellularmatrixandinterstitialcellphenotypeinpulmonaryvalves AT vikaskumar agerelatedextracellularmatrixandinterstitialcellphenotypeinpulmonaryvalves AT pengxiao agerelatedextracellularmatrixandinterstitialcellphenotypeinpulmonaryvalves AT mitchellkuss agerelatedextracellularmatrixandinterstitialcellphenotypeinpulmonaryvalves AT jungyullim agerelatedextracellularmatrixandinterstitialcellphenotypeinpulmonaryvalves AT chittibabuguda agerelatedextracellularmatrixandinterstitialcellphenotypeinpulmonaryvalves AT jonathanbutcher agerelatedextracellularmatrixandinterstitialcellphenotypeinpulmonaryvalves AT binduan agerelatedextracellularmatrixandinterstitialcellphenotypeinpulmonaryvalves
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Age related extracellular matrix and interstitial cell phenotype in pulmonary valves

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