A New Clade of Insect-Specific Flaviviruses from Australian <italic toggle="yes">Anopheles</italic> Mosquitoes Displays Species-Specific Host Restriction

ABSTRACT Flaviviruses are arthropod-borne viruses found worldwide and are responsible for significant human and veterinary diseases, including dengue, Zika, and West Nile fever. Some flaviviruses are insect specific and replicate only in mosquitoes. We report a genetically divergent group of insect-...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Agathe M. G. Colmant, Jody Hobson-Peters, Helle Bielefeldt-Ohmann, Andrew F. van den Hurk, Sonja Hall-Mendelin, Weng Kong Chow, Cheryl A. Johansen, Jelke Fros, Peter Simmonds, Daniel Watterson, Chris Cazier, Kayvan Etebari, Sassan Asgari, Benjamin L. Schulz, Nigel Beebe, Laura J. Vet, Thisun B. H. Piyasena, Hong-Duyen Nguyen, Ross T. Barnard, Roy A. Hall
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Anopheles insect-specific flavivirus coevolution dinucleotide analysis host restriction immunohistochemistry Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/8ec632591db04dedb9b88059f1d0663e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8ec632591db04dedb9b88059f1d0663e
record_format	dspace
spelling	oai:doaj.org-article:8ec632591db04dedb9b88059f1d0663e2021-11-15T15:22:04ZA New Clade of Insect-Specific Flaviviruses from Australian <italic toggle="yes">Anopheles</italic> Mosquitoes Displays Species-Specific Host Restriction10.1128/mSphere.00262-172379-5042https://doaj.org/article/8ec632591db04dedb9b88059f1d0663e2017-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00262-17https://doaj.org/toc/2379-5042ABSTRACT Flaviviruses are arthropod-borne viruses found worldwide and are responsible for significant human and veterinary diseases, including dengue, Zika, and West Nile fever. Some flaviviruses are insect specific and replicate only in mosquitoes. We report a genetically divergent group of insect-specific flaviviruses from Anopheles mosquitoes that do not replicate in arthropod cell lines or heterologous Anopheles species, exhibiting unprecedented specialization for their host species. Determination of the complete sequences of the RNA genomes of three of these viruses, Karumba virus (KRBV), Haslams Creek virus, and Mac Peak virus (McPV), that are found in high prevalence in some Anopheles mosquito populations and detection of virus-specific proteins, replicative double-stranded RNA, and small interfering RNA responses in the host mosquito species provided strong evidence of a functional replicating virus in the mosquito midgut. Analysis of nucleotide composition in the KRBV and McPV sequences also revealed a pattern consistent with the virus evolving to replicate only in insects. These findings represent a significant advance in our knowledge of mosquito-borne flavivirus ecology, host restriction, and evolution. IMPORTANCE Flaviviruses like dengue, Zika, or West Nile virus infect millions of people each year and are transmitted to humans via infected-mosquito bites. A subset of flaviviruses can only replicate in the mosquito host, and recent studies have shown that some can interfere with pathogenic flaviviruses in mosquitoes and limit the replication and transmission of the latter. The insect-specific flaviviruses (ISFs) reported here form a new Anopheles mosquito-associated clade separate from the Aedes- and Culex-associated ISF clades. The identification of distinct clades for each mosquito genus provides new insights into the evolution and ecology of flaviviruses. One of these viruses was shown to replicate in the midgut of the mosquito host and exhibit the most specialized host restriction reported to date for ISFs. Understanding this unprecedented host restriction in ISFs could help identify the mechanisms involved in the evolution of flaviviruses and their emergence as mosquito-borne pathogens.Agathe M. G. ColmantJody Hobson-PetersHelle Bielefeldt-OhmannAndrew F. van den HurkSonja Hall-MendelinWeng Kong ChowCheryl A. JohansenJelke FrosPeter SimmondsDaniel WattersonChris CazierKayvan EtebariSassan AsgariBenjamin L. SchulzNigel BeebeLaura J. VetThisun B. H. PiyasenaHong-Duyen NguyenRoss T. BarnardRoy A. HallAmerican Society for MicrobiologyarticleAnophelesinsect-specific flaviviruscoevolutiondinucleotide analysishost restrictionimmunohistochemistryMicrobiologyQR1-502ENmSphere, Vol 2, Iss 4 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Anopheles insect-specific flavivirus coevolution dinucleotide analysis host restriction immunohistochemistry Microbiology QR1-502
spellingShingle	Anopheles insect-specific flavivirus coevolution dinucleotide analysis host restriction immunohistochemistry Microbiology QR1-502 Agathe M. G. Colmant Jody Hobson-Peters Helle Bielefeldt-Ohmann Andrew F. van den Hurk Sonja Hall-Mendelin Weng Kong Chow Cheryl A. Johansen Jelke Fros Peter Simmonds Daniel Watterson Chris Cazier Kayvan Etebari Sassan Asgari Benjamin L. Schulz Nigel Beebe Laura J. Vet Thisun B. H. Piyasena Hong-Duyen Nguyen Ross T. Barnard Roy A. Hall A New Clade of Insect-Specific Flaviviruses from Australian <italic toggle="yes">Anopheles</italic> Mosquitoes Displays Species-Specific Host Restriction
description	ABSTRACT Flaviviruses are arthropod-borne viruses found worldwide and are responsible for significant human and veterinary diseases, including dengue, Zika, and West Nile fever. Some flaviviruses are insect specific and replicate only in mosquitoes. We report a genetically divergent group of insect-specific flaviviruses from Anopheles mosquitoes that do not replicate in arthropod cell lines or heterologous Anopheles species, exhibiting unprecedented specialization for their host species. Determination of the complete sequences of the RNA genomes of three of these viruses, Karumba virus (KRBV), Haslams Creek virus, and Mac Peak virus (McPV), that are found in high prevalence in some Anopheles mosquito populations and detection of virus-specific proteins, replicative double-stranded RNA, and small interfering RNA responses in the host mosquito species provided strong evidence of a functional replicating virus in the mosquito midgut. Analysis of nucleotide composition in the KRBV and McPV sequences also revealed a pattern consistent with the virus evolving to replicate only in insects. These findings represent a significant advance in our knowledge of mosquito-borne flavivirus ecology, host restriction, and evolution. IMPORTANCE Flaviviruses like dengue, Zika, or West Nile virus infect millions of people each year and are transmitted to humans via infected-mosquito bites. A subset of flaviviruses can only replicate in the mosquito host, and recent studies have shown that some can interfere with pathogenic flaviviruses in mosquitoes and limit the replication and transmission of the latter. The insect-specific flaviviruses (ISFs) reported here form a new Anopheles mosquito-associated clade separate from the Aedes- and Culex-associated ISF clades. The identification of distinct clades for each mosquito genus provides new insights into the evolution and ecology of flaviviruses. One of these viruses was shown to replicate in the midgut of the mosquito host and exhibit the most specialized host restriction reported to date for ISFs. Understanding this unprecedented host restriction in ISFs could help identify the mechanisms involved in the evolution of flaviviruses and their emergence as mosquito-borne pathogens.
format	article
author	Agathe M. G. Colmant Jody Hobson-Peters Helle Bielefeldt-Ohmann Andrew F. van den Hurk Sonja Hall-Mendelin Weng Kong Chow Cheryl A. Johansen Jelke Fros Peter Simmonds Daniel Watterson Chris Cazier Kayvan Etebari Sassan Asgari Benjamin L. Schulz Nigel Beebe Laura J. Vet Thisun B. H. Piyasena Hong-Duyen Nguyen Ross T. Barnard Roy A. Hall
author_facet	Agathe M. G. Colmant Jody Hobson-Peters Helle Bielefeldt-Ohmann Andrew F. van den Hurk Sonja Hall-Mendelin Weng Kong Chow Cheryl A. Johansen Jelke Fros Peter Simmonds Daniel Watterson Chris Cazier Kayvan Etebari Sassan Asgari Benjamin L. Schulz Nigel Beebe Laura J. Vet Thisun B. H. Piyasena Hong-Duyen Nguyen Ross T. Barnard Roy A. Hall
author_sort	Agathe M. G. Colmant
title	A New Clade of Insect-Specific Flaviviruses from Australian <italic toggle="yes">Anopheles</italic> Mosquitoes Displays Species-Specific Host Restriction
title_short	A New Clade of Insect-Specific Flaviviruses from Australian <italic toggle="yes">Anopheles</italic> Mosquitoes Displays Species-Specific Host Restriction
title_full	A New Clade of Insect-Specific Flaviviruses from Australian <italic toggle="yes">Anopheles</italic> Mosquitoes Displays Species-Specific Host Restriction
title_fullStr	A New Clade of Insect-Specific Flaviviruses from Australian <italic toggle="yes">Anopheles</italic> Mosquitoes Displays Species-Specific Host Restriction
title_full_unstemmed	A New Clade of Insect-Specific Flaviviruses from Australian <italic toggle="yes">Anopheles</italic> Mosquitoes Displays Species-Specific Host Restriction
title_sort	new clade of insect-specific flaviviruses from australian <italic toggle="yes">anopheles</italic> mosquitoes displays species-specific host restriction
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/8ec632591db04dedb9b88059f1d0663e
work_keys_str_mv	AT agathemgcolmant anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT jodyhobsonpeters anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT hellebielefeldtohmann anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT andrewfvandenhurk anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT sonjahallmendelin anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT wengkongchow anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT cherylajohansen anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT jelkefros anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT petersimmonds anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT danielwatterson anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT chriscazier anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT kayvanetebari anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT sassanasgari anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT benjaminlschulz anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT nigelbeebe anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT laurajvet anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT thisunbhpiyasena anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT hongduyennguyen anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT rosstbarnard anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT royahall anewcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT agathemgcolmant newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT jodyhobsonpeters newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT hellebielefeldtohmann newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT andrewfvandenhurk newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT sonjahallmendelin newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT wengkongchow newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT cherylajohansen newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT jelkefros newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT petersimmonds newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT danielwatterson newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT chriscazier newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT kayvanetebari newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT sassanasgari newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT benjaminlschulz newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT nigelbeebe newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT laurajvet newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT thisunbhpiyasena newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT hongduyennguyen newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT rosstbarnard newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction AT royahall newcladeofinsectspecificflavivirusesfromaustralianitalictoggleyesanophelesitalicmosquitoesdisplaysspeciesspecifichostrestriction
_version_	1718428083707445248

A New Clade of Insect-Specific Flaviviruses from Australian <italic toggle="yes">Anopheles</italic> Mosquitoes Displays Species-Specific Host Restriction

Ejemplares similares