Exosome-encapsulated antibiotic against intracellular infections of methicillin-resistant Staphylococcus aureus

Xiaohong Yang, Gongming Shi, Jian Guo, Chenhui Wang, Yun He Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, People’s Republic of China Background: Staphylococcus aureus survival inside pha...

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Autores principales:	Yang X, Shi G, Guo J, Wang C, He Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Exosomes Antibiotic Delivery Intracellular infection MRSA Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8ec8a5a2db9d41ccad70245a71cafa07
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spelling	oai:doaj.org-article:8ec8a5a2db9d41ccad70245a71cafa072021-12-02T09:50:43ZExosome-encapsulated antibiotic against intracellular infections of methicillin-resistant Staphylococcus aureus1178-2013https://doaj.org/article/8ec8a5a2db9d41ccad70245a71cafa072018-11-01T00:00:00Zhttps://www.dovepress.com/exosome-encapsulated-antibiotic-against-intracellular-infections-of-me-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xiaohong Yang, Gongming Shi, Jian Guo, Chenhui Wang, Yun He Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, People’s Republic of China Background: Staphylococcus aureus survival inside phagocytes is considered to provide a reservoir of bacteria that are relatively protected from antibiotics, thus enabling long-term colonization of the host and explaining clinical failures and relapses after antibiotic therapy. Purpose: The objective of this study was to develop a nanovesicle using exosomes loaded with linezolid to overcome intracellular infections by pathogenic bacteria. Methods: Exosomes were collected from the culture supernatants of RAW 264.7 cells. Their size distribution and zeta potential were characterized by dynamic light scattering, their morphology was characterized by transmission electron microscopy, and their protein content (CD63 and Flotillin 1) was assessed by Western blotting. Linezolid was incorporated into exosomes by co-incubation at 37°C and it’s accumulation in RAW264.7 cells and release in vitro were determined by high performance liquid chromatography. The intracellular bactericidal effect was evaluated in methicillin-resistant S. aureus (MRSA)-infected macrophages in vitro and MRSA peritonitis model in vivo. Results: We prepared a nanoformulation of the antibiotic linezolid using exosomes harvested from mouse RAW264.7 macrophages. The exosomal formulation of linezolid was more effective against intracellular MRSA infections in vitro and in vivo than the free linezolid. Our data also showed no signs of cytotoxicity in macrophages. Conclusion: Exosomes provide an effective alternative for intracellular antibiotic delivery of antibiotic that is efficacious, cost-effective, and safe. This regimen can be viewed as a potential antimicrobial agent for use against intracellular infections. Keywords: exosomes, antibiotic, delivery, intracellular infection, MRSAYang XShi GGuo JWang CHe YDove Medical PressarticleExosomesAntibioticDeliveryIntracellular infectionMRSAMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 8095-8104 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Exosomes Antibiotic Delivery Intracellular infection MRSA Medicine (General) R5-920
spellingShingle	Exosomes Antibiotic Delivery Intracellular infection MRSA Medicine (General) R5-920 Yang X Shi G Guo J Wang C He Y Exosome-encapsulated antibiotic against intracellular infections of methicillin-resistant Staphylococcus aureus
description	Xiaohong Yang, Gongming Shi, Jian Guo, Chenhui Wang, Yun He Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, People’s Republic of China Background: Staphylococcus aureus survival inside phagocytes is considered to provide a reservoir of bacteria that are relatively protected from antibiotics, thus enabling long-term colonization of the host and explaining clinical failures and relapses after antibiotic therapy. Purpose: The objective of this study was to develop a nanovesicle using exosomes loaded with linezolid to overcome intracellular infections by pathogenic bacteria. Methods: Exosomes were collected from the culture supernatants of RAW 264.7 cells. Their size distribution and zeta potential were characterized by dynamic light scattering, their morphology was characterized by transmission electron microscopy, and their protein content (CD63 and Flotillin 1) was assessed by Western blotting. Linezolid was incorporated into exosomes by co-incubation at 37°C and it’s accumulation in RAW264.7 cells and release in vitro were determined by high performance liquid chromatography. The intracellular bactericidal effect was evaluated in methicillin-resistant S. aureus (MRSA)-infected macrophages in vitro and MRSA peritonitis model in vivo. Results: We prepared a nanoformulation of the antibiotic linezolid using exosomes harvested from mouse RAW264.7 macrophages. The exosomal formulation of linezolid was more effective against intracellular MRSA infections in vitro and in vivo than the free linezolid. Our data also showed no signs of cytotoxicity in macrophages. Conclusion: Exosomes provide an effective alternative for intracellular antibiotic delivery of antibiotic that is efficacious, cost-effective, and safe. This regimen can be viewed as a potential antimicrobial agent for use against intracellular infections. Keywords: exosomes, antibiotic, delivery, intracellular infection, MRSA
format	article
author	Yang X Shi G Guo J Wang C He Y
author_facet	Yang X Shi G Guo J Wang C He Y
author_sort	Yang X
title	Exosome-encapsulated antibiotic against intracellular infections of methicillin-resistant Staphylococcus aureus
title_short	Exosome-encapsulated antibiotic against intracellular infections of methicillin-resistant Staphylococcus aureus
title_full	Exosome-encapsulated antibiotic against intracellular infections of methicillin-resistant Staphylococcus aureus
title_fullStr	Exosome-encapsulated antibiotic against intracellular infections of methicillin-resistant Staphylococcus aureus
title_full_unstemmed	Exosome-encapsulated antibiotic against intracellular infections of methicillin-resistant Staphylococcus aureus
title_sort	exosome-encapsulated antibiotic against intracellular infections of methicillin-resistant staphylococcus aureus
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/8ec8a5a2db9d41ccad70245a71cafa07
work_keys_str_mv	AT yangx exosomeencapsulatedantibioticagainstintracellularinfectionsofmethicillinresistantstaphylococcusaureus AT shig exosomeencapsulatedantibioticagainstintracellularinfectionsofmethicillinresistantstaphylococcusaureus AT guoj exosomeencapsulatedantibioticagainstintracellularinfectionsofmethicillinresistantstaphylococcusaureus AT wangc exosomeencapsulatedantibioticagainstintracellularinfectionsofmethicillinresistantstaphylococcusaureus AT hey exosomeencapsulatedantibioticagainstintracellularinfectionsofmethicillinresistantstaphylococcusaureus
_version_	1718398016700809216

Exosome-encapsulated antibiotic against intracellular infections of methicillin-resistant Staphylococcus aureus

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