The potential of cellulose-degrading fungi at various peat maturities in Teluk Bakung Peat Area, Kubu Raya District, Indonesia

Abstract. Khotimah S, Suharjono, Ardyati T, Nuraini Y. 2021. The potential of cellulose-degrading fungi at various peat maturities in Teluk Bakung Peat Area, Kubu Raya District, Indonesia. Biodiversitas 22: 1981-1990. Fungi play important roles in degrading cellulose in the maturation process of pea...

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Autores principales:	Siti Khotimah, Suharjono Suharjono, Tri Ardyati, Yulia Nuraini
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	peat fungi cellulase activity cmc media its region Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8ecae9c8c36b4567acfd2039aaf370e4
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spelling	oai:doaj.org-article:8ecae9c8c36b4567acfd2039aaf370e42021-11-22T00:57:38ZThe potential of cellulose-degrading fungi at various peat maturities in Teluk Bakung Peat Area, Kubu Raya District, Indonesia1412-033X2085-472210.13057/biodiv/d220446https://doaj.org/article/8ecae9c8c36b4567acfd2039aaf370e42021-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6967https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Khotimah S, Suharjono, Ardyati T, Nuraini Y. 2021. The potential of cellulose-degrading fungi at various peat maturities in Teluk Bakung Peat Area, Kubu Raya District, Indonesia. Biodiversitas 22: 1981-1990. Fungi play important roles in degrading cellulose in the maturation process of peat. This study aimed to evaluate the potential of cellulose-degrading fungi at various peat maturities in the forest. Peat soil samples were collected from Teluk Bakung Village, Kubu Raya District, West Kalimantan using a random sampling method. Peat soil samples were obtained at six sampling sites in forests and palm oil plantations. The fungi were cultured by the pour plate method on 1% CMC media. The cellulose-degrading fungi were determined based on a clear zone formation around the colony in CMC agar media. The cellulolytic fungal population in the fibric, hemic, and sapric peats of the secondary forest were 3.2 × 103, 4.6 × 103, and 5.6 × 103 CFU/g, respectively, whereas at fibric, hemic, and sapric of the shrubs were 5.6 x 104, 3.9 × 105 and 4.7 × 104 CFU/g, respectively. Among the 25 cellulolytic fungal isolates, 5 isolates (HS1.5, HS3.4.1, HB2.2, SS2.4.1, and SB2.5.1) showed high average clear zone diameter (21-28 mm). The HS3.4.1 and HS1.5 isolates had cellulase enzyme activity of 132.16 and 127.3 U/mL, respectively. These isolates can grow and degrade cellulose at 30°C to 35°C and pH of 2, 3, 4, and 5. The HS3.4.1 and HS1.5 isolate were closely related to Penicillium citrinum based on the phylogenetic tree analysis.Siti KhotimahSuharjono SuharjonoTri ArdyatiYulia NurainiMBI & UNS Soloarticlepeatfungicellulase activitycmc mediaits regionBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	peat fungi cellulase activity cmc media its region Biology (General) QH301-705.5
spellingShingle	peat fungi cellulase activity cmc media its region Biology (General) QH301-705.5 Siti Khotimah Suharjono Suharjono Tri Ardyati Yulia Nuraini The potential of cellulose-degrading fungi at various peat maturities in Teluk Bakung Peat Area, Kubu Raya District, Indonesia
description	Abstract. Khotimah S, Suharjono, Ardyati T, Nuraini Y. 2021. The potential of cellulose-degrading fungi at various peat maturities in Teluk Bakung Peat Area, Kubu Raya District, Indonesia. Biodiversitas 22: 1981-1990. Fungi play important roles in degrading cellulose in the maturation process of peat. This study aimed to evaluate the potential of cellulose-degrading fungi at various peat maturities in the forest. Peat soil samples were collected from Teluk Bakung Village, Kubu Raya District, West Kalimantan using a random sampling method. Peat soil samples were obtained at six sampling sites in forests and palm oil plantations. The fungi were cultured by the pour plate method on 1% CMC media. The cellulose-degrading fungi were determined based on a clear zone formation around the colony in CMC agar media. The cellulolytic fungal population in the fibric, hemic, and sapric peats of the secondary forest were 3.2 × 103, 4.6 × 103, and 5.6 × 103 CFU/g, respectively, whereas at fibric, hemic, and sapric of the shrubs were 5.6 x 104, 3.9 × 105 and 4.7 × 104 CFU/g, respectively. Among the 25 cellulolytic fungal isolates, 5 isolates (HS1.5, HS3.4.1, HB2.2, SS2.4.1, and SB2.5.1) showed high average clear zone diameter (21-28 mm). The HS3.4.1 and HS1.5 isolates had cellulase enzyme activity of 132.16 and 127.3 U/mL, respectively. These isolates can grow and degrade cellulose at 30°C to 35°C and pH of 2, 3, 4, and 5. The HS3.4.1 and HS1.5 isolate were closely related to Penicillium citrinum based on the phylogenetic tree analysis.
format	article
author	Siti Khotimah Suharjono Suharjono Tri Ardyati Yulia Nuraini
author_facet	Siti Khotimah Suharjono Suharjono Tri Ardyati Yulia Nuraini
author_sort	Siti Khotimah
title	The potential of cellulose-degrading fungi at various peat maturities in Teluk Bakung Peat Area, Kubu Raya District, Indonesia
title_short	The potential of cellulose-degrading fungi at various peat maturities in Teluk Bakung Peat Area, Kubu Raya District, Indonesia
title_full	The potential of cellulose-degrading fungi at various peat maturities in Teluk Bakung Peat Area, Kubu Raya District, Indonesia
title_fullStr	The potential of cellulose-degrading fungi at various peat maturities in Teluk Bakung Peat Area, Kubu Raya District, Indonesia
title_full_unstemmed	The potential of cellulose-degrading fungi at various peat maturities in Teluk Bakung Peat Area, Kubu Raya District, Indonesia
title_sort	potential of cellulose-degrading fungi at various peat maturities in teluk bakung peat area, kubu raya district, indonesia
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/8ecae9c8c36b4567acfd2039aaf370e4
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The potential of cellulose-degrading fungi at various peat maturities in Teluk Bakung Peat Area, Kubu Raya District, Indonesia

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