Transcriptome analysis reveals long intergenic non-coding RNAs involved in skeletal muscle growth and development in pig

Abstract Long intergenic non-coding RNAs (lincRNAs) play essential roles in numerous biological processes and are widely studied. The skeletal muscle is an important tissue that plays an essential role in individual movement ability. However, lincRNAs in pig skeletal muscles are largely undiscovered...

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Autores principales:	Cheng Zou, Jingxuan Li, Wenzhe Luo, Long Li, An Hu, Yuhua Fu, Ye Hou, Changchun Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8edb89db90a04aad928b7a737a407a45
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spelling	oai:doaj.org-article:8edb89db90a04aad928b7a737a407a452021-12-02T12:31:46ZTranscriptome analysis reveals long intergenic non-coding RNAs involved in skeletal muscle growth and development in pig10.1038/s41598-017-07998-92045-2322https://doaj.org/article/8edb89db90a04aad928b7a737a407a452017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07998-9https://doaj.org/toc/2045-2322Abstract Long intergenic non-coding RNAs (lincRNAs) play essential roles in numerous biological processes and are widely studied. The skeletal muscle is an important tissue that plays an essential role in individual movement ability. However, lincRNAs in pig skeletal muscles are largely undiscovered and their biological functions remain elusive. In this study, we assembled transcriptomes using RNA-seq data published in previous studies of our laboratory group and identified 323 lincRNAs in porcine leg muscle. We found that these lincRNAs have shorter transcript length, fewer exons and lower expression level than protein-coding genes. Gene ontology and pathway analyses indicated that many potential target genes (PTGs) of lincRNAs were involved in skeletal-muscle-related processes, such as muscle contraction and muscle system process. Combined our previous studies, we found a potential regulatory mechanism in which the promoter methylation of lincRNAs can negatively regulate lincRNA expression and then positively regulate PTG expression, which can finally result in abnormal phenotypes of cloned piglets through a certain unknown pathway. This work detailed a number of lincRNAs and their target genes involved in skeletal muscle growth and development and can facilitate future studies on their roles in skeletal muscle growth and development.Cheng ZouJingxuan LiWenzhe LuoLong LiAn HuYuhua FuYe HouChangchun LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cheng Zou Jingxuan Li Wenzhe Luo Long Li An Hu Yuhua Fu Ye Hou Changchun Li Transcriptome analysis reveals long intergenic non-coding RNAs involved in skeletal muscle growth and development in pig
description	Abstract Long intergenic non-coding RNAs (lincRNAs) play essential roles in numerous biological processes and are widely studied. The skeletal muscle is an important tissue that plays an essential role in individual movement ability. However, lincRNAs in pig skeletal muscles are largely undiscovered and their biological functions remain elusive. In this study, we assembled transcriptomes using RNA-seq data published in previous studies of our laboratory group and identified 323 lincRNAs in porcine leg muscle. We found that these lincRNAs have shorter transcript length, fewer exons and lower expression level than protein-coding genes. Gene ontology and pathway analyses indicated that many potential target genes (PTGs) of lincRNAs were involved in skeletal-muscle-related processes, such as muscle contraction and muscle system process. Combined our previous studies, we found a potential regulatory mechanism in which the promoter methylation of lincRNAs can negatively regulate lincRNA expression and then positively regulate PTG expression, which can finally result in abnormal phenotypes of cloned piglets through a certain unknown pathway. This work detailed a number of lincRNAs and their target genes involved in skeletal muscle growth and development and can facilitate future studies on their roles in skeletal muscle growth and development.
format	article
author	Cheng Zou Jingxuan Li Wenzhe Luo Long Li An Hu Yuhua Fu Ye Hou Changchun Li
author_facet	Cheng Zou Jingxuan Li Wenzhe Luo Long Li An Hu Yuhua Fu Ye Hou Changchun Li
author_sort	Cheng Zou
title	Transcriptome analysis reveals long intergenic non-coding RNAs involved in skeletal muscle growth and development in pig
title_short	Transcriptome analysis reveals long intergenic non-coding RNAs involved in skeletal muscle growth and development in pig
title_full	Transcriptome analysis reveals long intergenic non-coding RNAs involved in skeletal muscle growth and development in pig
title_fullStr	Transcriptome analysis reveals long intergenic non-coding RNAs involved in skeletal muscle growth and development in pig
title_full_unstemmed	Transcriptome analysis reveals long intergenic non-coding RNAs involved in skeletal muscle growth and development in pig
title_sort	transcriptome analysis reveals long intergenic non-coding rnas involved in skeletal muscle growth and development in pig
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8edb89db90a04aad928b7a737a407a45
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Transcriptome analysis reveals long intergenic non-coding RNAs involved in skeletal muscle growth and development in pig

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