A call for caution in analysing mammalian co-transfection experiments and implications of resource competition in data misinterpretation

Transient transfections are routinely used in basic and synthetic biology studies to unravel pathway regulation and to probe and characterise circuit designs. As each experiment has a component of intrinsic variability, reporter gene expression is usually normalized with co-delivered genes that act...

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Autores principales:	Roberto Di Blasi, Masue M. Marbiah, Velia Siciliano, Karen Polizzi, Francesca Ceroni
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/8edf6ae5ac17436485b407b1d9cf9f05
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spelling	oai:doaj.org-article:8edf6ae5ac17436485b407b1d9cf9f052021-12-02T14:49:23ZA call for caution in analysing mammalian co-transfection experiments and implications of resource competition in data misinterpretation10.1038/s41467-021-22795-92041-1723https://doaj.org/article/8edf6ae5ac17436485b407b1d9cf9f052021-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-22795-9https://doaj.org/toc/2041-1723Transient transfections are routinely used in basic and synthetic biology studies to unravel pathway regulation and to probe and characterise circuit designs. As each experiment has a component of intrinsic variability, reporter gene expression is usually normalized with co-delivered genes that act as transfection controls. Recent reports in mammalian cells highlight how resource competition for gene expression leads to biases in data interpretation, with a direct impact on co-transfection experiments. Here we define the connection between resource competition and transient transfection experiments and discuss possible alternatives. Our aim is to raise awareness within the community and stimulate discussion to include such considerations in future experimental designs, for the development of better transfection controls.Roberto Di BlasiMasue M. MarbiahVelia SicilianoKaren PolizziFrancesca CeroniNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Roberto Di Blasi Masue M. Marbiah Velia Siciliano Karen Polizzi Francesca Ceroni A call for caution in analysing mammalian co-transfection experiments and implications of resource competition in data misinterpretation
description	Transient transfections are routinely used in basic and synthetic biology studies to unravel pathway regulation and to probe and characterise circuit designs. As each experiment has a component of intrinsic variability, reporter gene expression is usually normalized with co-delivered genes that act as transfection controls. Recent reports in mammalian cells highlight how resource competition for gene expression leads to biases in data interpretation, with a direct impact on co-transfection experiments. Here we define the connection between resource competition and transient transfection experiments and discuss possible alternatives. Our aim is to raise awareness within the community and stimulate discussion to include such considerations in future experimental designs, for the development of better transfection controls.
format	article
author	Roberto Di Blasi Masue M. Marbiah Velia Siciliano Karen Polizzi Francesca Ceroni
author_facet	Roberto Di Blasi Masue M. Marbiah Velia Siciliano Karen Polizzi Francesca Ceroni
author_sort	Roberto Di Blasi
title	A call for caution in analysing mammalian co-transfection experiments and implications of resource competition in data misinterpretation
title_short	A call for caution in analysing mammalian co-transfection experiments and implications of resource competition in data misinterpretation
title_full	A call for caution in analysing mammalian co-transfection experiments and implications of resource competition in data misinterpretation
title_fullStr	A call for caution in analysing mammalian co-transfection experiments and implications of resource competition in data misinterpretation
title_full_unstemmed	A call for caution in analysing mammalian co-transfection experiments and implications of resource competition in data misinterpretation
title_sort	call for caution in analysing mammalian co-transfection experiments and implications of resource competition in data misinterpretation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8edf6ae5ac17436485b407b1d9cf9f05
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A call for caution in analysing mammalian co-transfection experiments and implications of resource competition in data misinterpretation

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